The diagram below shows a section of double-stranded DNA undergoing both transcription and replication. RNA polymerase (gray oval) is bound to the transcriptional template strand and moving from left to right (arrow). The resulting RNA transcript is also shown (dotted line) with limited base pairing to the template strand. The DNA sequence is specified for a portion of the double-stranded DNA. E A ATGC ТАCG RNA transcription DNA replication a. Indicate whether point C is a 5' end or a 3' end of a nucleic acid. b. Indicate which strand (upper or lower) is the template for lagging strand synthesis c. Indicate the nucleotide sequence of the RNA that was transcribed from the DNA region specified by the sequence. Label the

For each mutant, state what change has occurred in the DNA, whether it was a substitution by transition or transversion, sense mutation, nonsense or reading frame change. It must present the codon sequence. Normal nucleotide sequence starting from the third codon: CCC-ACG-GUG-ACG-ACA-CGG-UGG Please show the codon and nucleotide sequence of the mutation.

The following sequence of DNA is part of the normal, wild-type gene.   5'TAC CGG GAC TTG AGC CGA TAG 3'   A deletion occurs during DNA replication, causing the guanine shown in red to be removed from the nucleotide strand. What effect is this most likely to have on the final protein?   Multiple Choice   The deletion of the G will cause a single amino acid substitution in the codon in which it occurs.   The deletion of the G will cause a frameshift, so that the first amino acid after the mutation will change but the rest of the protein is unaffected.   The deletion of the G will cause a frameshift, resulting in a premature stop codon and a truncated protein.   The deletion of the G will cause a frameshift, resulting in the loss of the normal stop codon and an abnormally long protein with an altered amino acid sequence.   The deletion of the G will not have an effect on the final protein.

Are these true or false answers correct? If so, what is the proper word to replace the underlined words, for the false.

During translation, the formation of peptide bonds between adjacent amino acids requires peptidyl transferase enzyme activity, which is carried out by ______________________.   Release Factor 23S rRNA of the large ribosomal subunit 16S rRNA of the small ribosomal subunit tRNA A protein enzyme called peptidyl transferase

The most likely and immediate affect of the deletion of the Shine-Dalgarno sequence would be: Group of answer choices initiation of replication will not take place 50S subunit cannot form the initiation complex ribosomes will be unable to bind to mRNA mRNA will degrade more rapidly

More than one answer can be correct

Listed below are five amino acids. Use the genetic code to determine the exact codon for each amino acid. A point mutation at the genetic level in each codon results in the change indicated. For each mutation, indicate whether it is due to a transition or a transversion, and then indicate the effect of each mutation at the protein (amino acid level) (i.e. silent, nonsense, missense). In addition, Please note, each of the three lines above an amino acid represents a single RNA base. For example, when you look at the codon chart AUG would stand for Met (methionine) Lys 1 Glu Ile 3 Stop Ile 4.

A polypeptide has the following amino acid sequence: Met-Ser-Pro-Arg-Leu-Glu-Gly The amino acid sequence of this polypeptide was determined in a series of mutants listed in parts a through e.  indicate the type of mutation that occurred in the DNA (single-base substitution, insertion, deletion) and the phenotypic effect of the mutation (nonsense mutation, missense mutation, frameshift, etc.). a. MMutant 4: Met-Ser-Pro-Glu-Gl

Match the term with its definition comparing genomic DNA, mRNA, and proteins. You may only use each option ONCE. where transcription starts. where translation ends a chemical group that indicates the first nucleotide that was added to the mRNA a chemical group that indicates the first amino acid that was added to the polypeptide a DNA sequence that is neither transcribed nor translated a non-protein coding region upstream of the start codon in the mRNA promoter complimentary base-pairs with the codon amino-terminus a DNA sequence that is transcribed, but not intron translated [Choose ] stop codon +1 site tRNA 5-prime UTR start codon exon 5-prime triphosphate promoter amino-terminus 5-prime triphosphate stop codon