Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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- Under standard conditions, will the following reactions proceed spontaneously as written? (1) Fumarate + NADH + H+ (2) succinate + NAD+ Cyto a (Fe²+) + cyto b (Fe³+) = cyto a (Fe³+) + cyto 6 (Fe²+) barrow_forwardConsider the fructose-1,6-bisphosphatase reaction. Calculate the free energy change if the ratio of the concentrations of the products to the concentrations of the reactants is 23.7, and the temperature is 37.0 °C? AG" for the reaction is -16.7 kJ/mol. The constant R = 8.3145 J/(mol-K) AG= Number kJ/molarrow_forwardBelow is an image of the Krebs cycle: acetyl-CoA oxaloacetate COASH H20 NADH NAD* H20 malate citrate fumarate isocitrate FADH2 NAD* CO2 FAD АТР NADH + ADP succinate GTP NAD+ a-ketoglutarate H20 GDP NADH + CO2 COASH succinyl CoA COASH Consider the conversion of succinate to fumarate, which is coupled with the production the electron carrier FADH2. If this reaction was NOT coupled with the production of FADH2 (and only catalyzed the conversion of succinate to fumarate), how would this impact ATP production through cell respiration? OATP production would stop because no high energy electron carriers would be produced ATP production would still occur, but there would be a much lower ATP yield because a large number of electron carriers are no longer being made ATP production would stop because without FADH2 we will no longer have electrons moving through the electron transport chain ATP production would still occur, but there would be a slightly lower ATP yield because a small number of…arrow_forward
- Write a balanced equation for the complete metabolic oxidation of each of the following. Include O, ADP, and P; as reactants and ATP, CO, and H;0 as products. (a) Stearic acid (b) Oleic acid (c) Palmitic acid (d) Linoleic acidarrow_forwardFree energy changes under intracellular conditions differ markedly from those determined under standard conditions. ΔG°′ = -32.2 kJ/mol for ATP hydrolysis to ADP and Pi. Calculate ΔG for ATP hydrolysis in a cell at 37 °C that contains [ATP] = 3 mM, [ADP] = 1 mM, and 3Pi4 = 1 mM.arrow_forwardCalculate the number of ATPATP generated from one saturated 1212‑carbon fatty acid. Assume that each NADHNADH molecule generates 2.5 ATP2.5 ATP and that each FADH2FADH2 molecule generates 1.5 ATP1.5 ATP .arrow_forward
- Consider the following equilibrium at 298 K. R=8.314 J/K-mol Acetyl-CoA + Oxaloacetate + H20 Citrate + COASH + H* Which one of the following is the correct value for K'eg ifAG" = -32.2 kJ/mol? O 1.11 0.987 1.01 x 10-13 13.0 9.92 x 1012 2480 4.42 x 105 2.27 x 10-6 1.01 -2480 2.56 -13.0 1.08 -0.0130 0.0130 0.926arrow_forwardBy what factor is sample A2 more concentrated than sample A1?arrow_forwardConsider the following reaction of the glycolysis pathway. Which statement is NOT true regarding this reaction? P + C-H NADH + H+ NAD+ C-0-P H-C-OH H-C-OH Glyceraldehyde- CH₂0-P 3-phosphate dehydrogenase CH₂0-P 1,3-Bisphosphoglycerate Glyceraldehyde-3- phosphate O A one-carbon compound (1C) is converted into a two-carbon compound (2C). O It requires a coenzyme. O It is a redox reaction catalyzed by the enzyme glyceraldehyde-3-phosphate dehydrogenase. O It is a phosphorylation reaction.arrow_forward
- Calculate the ΔG for Malate dehydrogenase reaction of CAC if the concentration of oxaloacetate is 1x 10-8 M, malate is 0.2 mM, NAD+ is 10 mM and NADH is 0.1 mM in rat liver mitochondria. ΔGo’ for this reaction is 30 kJ/mole. Please write the units of the final answer and the formula you are using to solve. R = 0.008314kJ/K*mol, T = 298Karrow_forwardGlyceraldehyde-3-phosphate (GAP) is converted to 1,3-bisphosphoglycerate (1,3-BPG) as shown. GAP + P + NAD+ 1,3-BPG + NADH Is this reaction spontaneous? How do you know? AG = 6.7 kJ mol-¹ The reaction shown above is coupled to the following reaction in which 1,3-BPG is converted to 3-phosphoglycerate (3-PG): GAP + P + NAD+ + ADP3-PG + NADH + ATP AG = -18.8 kJ mol-¹ 1,3-BPG+ADP3-PG+ATP Write the equation for the overall conversion of GAP to 3PG. Is the coupled reaction favorable? How do you know?arrow_forward
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