Glyceraldehyde-3-phosphate (GAP) is converted to 1,3-bisphosphoglycerate (1,3-BPG) as shown. GAP + P + NAD+ 1,3-BPG + NADH Is this reaction spontaneous? How do you know? AG = 6.7 kJ mol-¹ The reaction shown above is coupled to the following reaction in which 1,3-BPG is converted to 3-phosphoglycerate (3-PG):

Consider the malate dehydrogenase reaction from the citric acid cycle. Given the listed concentrations, calculate the free energy change for this reaction at energy change for this reaction at 37.0 ˚C (310 K). AG' for the reaction is +29.7 kJ/mol. Assume that the reaction occurs at pH 7. [malate] = 1.45 mM AG: [oxaloacetate] = 0.130 mM [NAD+ ] = 110 mM [NADH] = 44 mM 47.06 Incorrect kJ.mol-1

How does one plot the lineweaver burk plot using this data.

During glycolysis, glucose is converted into fructose-6- phosphate in two successive reactions: glucose + ATP glucose 6-phosphate + ADP glucose 6-phosphate fructose 6-phosphate The AG' for the overall reaction is: +15.0 kJ/mol. -18.4 kJ/mol. -16.7 kJ/mol. → -15.0 kJ/mol. +18.4 kJ/mol. AG¹⁰ = -16.7 kJ/mol AG¹⁰ = +1.7 kJ/mol

Refer to the figure shown here, and determine the value of E for the overall oxidation/reduction reaction (refer to the book/lecture slides if you need help with the overall reaction). 121/202 + 2H+ + 2e → H₂0 NAD + H* + 2e → NADH O-1.136 volts O 0.496 volts O+1.136 volts voltsm -0.496 volts EU (volts) +0.816 - 0.320

Which of the reactions are spontaneous (favorable)? C6H130,P + ATP → › C6H14º₁₂P2 + ADP AG = -14.2 kJ/mol L-malate + NAD+ → oxaloacetate + NADH + H+ AG = 29.7 kJ/mol glutamate + NAD+ + H₂O → NH‡ + α-ketoglutarate + NADH + H+ AG = 3.7 kcal/mol → CH2O4 + H2O AG = 3.1 kJ/mol * CąHẠO, — CH,O4 + H,O DHAP C₂H + H₂ glyceraldehyde-3-phosphate AG = 3.8 kJ/mol Rh(I) C2H6 AG-150.97 kJ/mol

Determine whether the following reactions will be spontaneous under standard biochemical conditions. Include calculations for Delta G in your answer. Use Table 13-7 in your textbook to help you.a) 2NADH + 2H+ + O2 --> 2NAD+ + 2H2Ob) Malate + FAD --> Oxaloacetate + FADH2c) Pyruvate + H2S --> Lactate + S

Refer to Table to explain why FAD rather than NAD+ is used in the succinate dehydrogenase reaction.

For the following enzymes (3-6) predict how the conditions will most likely affect the enzymes activity with one of the following and provide a 1 sentence explanation: a. increase activity                     b. decrease activity                             c. not likely to alter activity  3) alpha-ketoglutarate dehydrogenase complex binding to AMP when [AMP] is high. 4) phosphohexose isomerase binding NADH when [NADH] is high 5) phosphofructose-1 binding NAD+ when [NAD+] is high  6) pyruvate dehydrogenase complex binding to ATP when [ATP] is high

The oxidation of Gly-3-P to 1,3-bisphosphoglycerate proceeds with an unfavorable AG which equals 6.3 KJ/mol, yet the flow through this point in the glycolytic pathway proceeds smoothly. Why???