Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
Bartleby Related Questions Icon

Related questions

Question

By what factor is sample A2 more concentrated than sample A1? 

8. Below, typical UV absorption spectra for NAD and NADH are shown.
In a spectroscopic experiment where the presence of an analyte A is directly linked to the enzyme catalysed generation of NADH, the following absorbance values were observed at a wavelength of 340
nm in samples containing two different concentrations of A:
Absorbance, BLANK (sum of all components): 0.2
Absorbance, SAMPLE A1 low conc: 0.4
Absorbance, SAMPLE A2 high conc: 0.8
1.0
Oxidized
(NAD*)
0.8
0.6
Reduced
(NADH)
0.4
0.2
0.0
220 240
260 280 300 320 340 360 380
Wavelength (nm)
By what factor is SAMPLE A2 more concentrated than SAMPLE A1?
Note Absorbance values are additive and directly proportional to concentrations.
Absorbance
expand button
Transcribed Image Text:8. Below, typical UV absorption spectra for NAD and NADH are shown. In a spectroscopic experiment where the presence of an analyte A is directly linked to the enzyme catalysed generation of NADH, the following absorbance values were observed at a wavelength of 340 nm in samples containing two different concentrations of A: Absorbance, BLANK (sum of all components): 0.2 Absorbance, SAMPLE A1 low conc: 0.4 Absorbance, SAMPLE A2 high conc: 0.8 1.0 Oxidized (NAD*) 0.8 0.6 Reduced (NADH) 0.4 0.2 0.0 220 240 260 280 300 320 340 360 380 Wavelength (nm) By what factor is SAMPLE A2 more concentrated than SAMPLE A1? Note Absorbance values are additive and directly proportional to concentrations. Absorbance
Expert Solution
Check Mark
Knowledge Booster
Background pattern image
Similar questions
SEE MORE QUESTIONS
Recommended textbooks for you
Text book image
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Text book image
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Text book image
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Text book image
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Text book image
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Text book image
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education