By what factor is sample A2 more concentrated than sample A1? 8. Below, typical UV absorption spectra for NAD and NADH are shown.In a spectroscopic experiment where the presence of an analyte A is directly linked to the enzyme catalysed generation of NADH, the following absorbance values were observed at a wavelength of 340nm in samples containing two different concentrations of A:Absorbance, BLANK (sum of all components): 0.2Absorbance, SAMPLE A1 low conc: 0.4Absorbance, SAMPLE A2 high conc: 0.81.0Oxidized(NAD*)0.80.6Reduced(NADH)0.40.20.0220 240260 280 300 320 340 360 380Wavelength (nm)By what factor is SAMPLE A2 more concentrated than SAMPLE A1?Note Absorbance values are additive and directly proportional to concentrations.Absorbance

The wavelength of the analyte A is 340nm The absorbance value is; A1= 0.4 A2 = 0.8 The relation of…

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By what factor is sample A2 more concentrated than sample A1?

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question

By what factor is sample A2 more concentrated than sample A1?

8. Below, typical UV absorption spectra for NAD and NADH are shown.
In a spectroscopic experiment where the presence of an analyte A is directly linked to the enzyme catalysed generation of NADH, the following absorbance values were observed at a wavelength of 340
nm in samples containing two different concentrations of A:
Absorbance, BLANK (sum of all components): 0.2
Absorbance, SAMPLE A1 low conc: 0.4
Absorbance, SAMPLE A2 high conc: 0.8
1.0
Oxidized
(NAD*)
0.8
0.6
Reduced
(NADH)
0.4
0.2
0.0
220 240
260 280 300 320 340 360 380
Wavelength (nm)
By what factor is SAMPLE A2 more concentrated than SAMPLE A1?
Note Absorbance values are additive and directly proportional to concentrations.
Absorbance

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