What types of samples can be measured with Circular Dichroism? Can information be extracted?
Q: What are the expected problems in the centrifuge in case of imbalance of samples ? *
A: Centrifugation is a process of separation of molecules from mixtures by applying centrifugal force…
Q: What advantages do solid media offer for the isolation ofmicroorganisms?
A: Micro-organism are those that cannot be seen by the naked eye, but are visible under microscopes.…
Q: How does incubating plates at 37°C influence the results of the quantitative dilution and plating of…
A: Under the regulated conditions of the laboratory when microbial cells are allowed to grow and…
Q: How might storage of the coconut water samples at room temperature after collection and before…
A: Under the regulated conditions of the laboratory when microbial cells are allowed to grow and…
Q: Explain why only gram-negative cells undergo decolorization during the gram staining procedure.
A: Gram staining is a method in which cells stain either pink (gram-negative) or purple (gram-positive)…
Q: Why is dilution a necessary part of pure culture preparation?
A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…
Q: How to count the number of colonies forming units per milliliter (CFUs per ml) of the sample?
A: A viable cell is one that has the ability to divide and form a colony. The colony-forming unit (CFU)…
Q: What is the difference between Dry and Wet Celloidin Method?
A: Celloidin method is a histopathological technique of tissue impregnation and embedding that helps to…
Q: In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside…
A: Several laboratory techniques necessitate smear preparation. A smear can be made using either broth…
Q: Why is it important to keep the culture at the correct incubation temperature when measuring the…
A: Generation time is the time which is taken by the bacteria to double in number during a specified…
Q: Describe how the streak-plate method is used to obtain a pure culture and how the resulting culture…
A: In microbiology, culture refers to microbial growth of a certain species maintained in controlled…
Q: What are the disadvantages of doing a total microbial count by examining wet mounts under the…
A: Ans- Wet mounts have the following drawbacks: The benefit of being able to observe movement can also…
Q: Why is it sufficient to stain cells from a SDA (Sabouraud Dextrose Agar) plate with crystal violet…
A: Introduction : Sabouraud agar, often known as Sabouraud dextrose agar (SDA), is a peptone-containing…
Q: Which of the following cannot be grown in artificial media and must be grown in live animals or…
A: Artificial media is a medium that is defined. This medium is chemically defined. It is a synthetic…
Q: What are pure cultures and why are they important? How are spread plates, streak plates, and pour…
A: In science and related fields, a culture plate is a low level lined lab holder for growing a layer…
Q: What concentration of agar is used in a semisolid medium for motility determination?
A: Motility is a feature characteristic of only some bacteria. This ability allows the bacteria to move…
Q: If you transfer 0.1 mL of culture into a 99mL of sterile water, then add 1mL of that to an agar…
A: Making a solution thinner or less dense is the procedure of dilution. Serial dilutions (also known…
Q: The factors for the manipulation of culture medium?
A: Culture medium It is also called a growth medium. It is a solid, liquid, or semi-solid medium that…
Q: If you conducted an acid-fast stain on a sputum sample from a person with tuberculosis, what would…
A: The acid-fast staining technique is a differential staining technique. The bacteria which get…
Q: What are the purposes of carrying out the streak plate andpour plate techniques in microbiology?
A: It is a technique used to isolate a pure strain from a single species of microorganism, often…
Q: What are high level disinfectants? What is an example of one? What is the method of action and the…
A: Disinfectants are chemicals that are used to kill the microorganisms present in the surroundings.…
Q: temperature and light source affect the number and type of colonies that can be found in the…
A: Microbes such as bacteria, fungi, viruses are grown at the earth's surface. The atmosphere provides…
Q: How is the correct way of incubating culture plate media? Explain why is that so.
A: After the way of life medium is set, and streaked with the necessary microbe/stock, the cover is put…
Q: What is one advantage of a pour plate over a streak plate?
A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…
Q: What is a pure culture and how can one be obtained
A: Cells are the structural and functional units of life.
Q: What is the use of hay infusion in determination of microbial motility?
A: Hay infusion use in determination of microbial motility.
Q: What makes agar good support for microbial growth?
A: Microbial culture is a method of multiplying microbial organisms by letting them reproduce in…
Q: What is the advantage of the plating method over an electronic cell counting method in counting…
A: Bacteria culturing is done in order to grow the bacterial strains that are able to show resistance…
Q: Can simply observing the colonies formed on agar plates and the results of a few biochemical tests…
A: These are biochemical test is to perform to identify different type of bacteria.
Q: mportance of pH adjustment in culture media?
A: Culture media is also known as growth media. It is a mixture of nutrients that helps for the growth…
Q: Can simply observing the colonies formed on agar plates and the results of a few biochemical tests…
A: Bacteria from colonies in the agar plates and different bacterial colonies will differ in shape,…
Q: What is the main difference between plates before and after centrifugation? What may be the purpose…
A: Answer: CENTRIFUGATION = It is the process involving mechanical force that is centrifugal force,…
Q: What is the nature of samples that should NOT be placed inside the drying-oven?
A: According to the question, we have to explain the nature of the samples that should not be placed…
Q: Of what practical importance are air borne microorganisms to the laboratory workers? What…
A:
Q: In Gram staining method, list the reagents and state their role for each step of the procedure? In…
A: Bacteria are unicellular, prokaryotic organicism which are devoid of membrane bound organelles and a…
Q: How might incubating plates at 37°C influence the results of the quantitative dilution and plating…
A: Ideal conditions are essential for the growth and reproduction of organisms including bacteria.…
Q: How does the age of a culture affect the Gram stain reaction? What is an optimum culture age for a…
A: To identify the unknown bacteria this gram staining is used. This staining technique differentiate…
Q: What are the culture media that can be used for cultivating non-specific and specific…
A: A microbiological culture medium is a substance that promotes the growth, support, and survival of…
Q: what is the procedure of solving the probelems of cultures those are suffering severely due to…
A: This is the defense compound of the plant and are toxic in nature because of that preventing plant…
Q: What type of agar is MacConkey agar?
A: MacConkey agar or MAC which is also described as a bacterial culture medium was named after the…
Q: Why is egg enrichment not added to BPA prior to sterilization?
A: Baird–parker agar or BPA is a selective medium is identify or isolate S. aureus in food. It contains…
Q: In disc diffusion studies, where will the lowest concentration of the tested chemical be in the agar…
A: The disc diffusion method was developed by Kirby and Bauer to determine the efficiency of a…
Q: Why is it necessary to cut thin sections of the tissue sample from a specimen using a microtome?
A: Microtomes are specialized cutting instruments that produce very thin slices of tissues so that the…
Q: What products are sterilized using high energy ionizing radiation?
A: Sterilization is the process of killing microorganisms.
Q: What is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary…
A: Need to explain the primary stain in the Kinyoun acid-fast stain.
Q: What is the major difference between Thayer-Martin and chocolate agar? When would you use…
A:
What types of samples can be measured with Circular Dichroism? Can information be extracted?
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- Describe how the streak-plate method is used to obtain a pure culture and how the resulting culture can be stored.What advantages do solid media offer for the isolation of microorganisms? How will you check the purity of an isolate?What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?
- In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable to use refrigerated samples for this procedure Why or Why not?What are pure cultures and why are they important? How are spread plates, streak plates, and pour plates prepared?What is the main difference between plates before and after centrifugation? What may be the purpose of concentrating the media and perform a second plating?
- What type of media are culture medium 1 and culture medium 2? And its difference to each other?In Microbiology What does a metallic green sheen indicate on an EMB (Eosin Methylene Blue agar)plate? How is this metallic green sheen formed?Why does a streaking method used to inoculate plates result in isolated colonies? (Explain systematically)