Why is it important to keep the culture at the correct incubation temperature when measuring the generation time?

I have a culture containing 106 organisms/ml and need to dilute it to 1000 organisms/ ml.    Can you draw out a serial dilution diagram that I could follow to reach 1000 organisms/ ml If I plate 150ul of the final diultion onto YPD media, how many colonies approx would be expected on the plate?

Create a graph of this spectrophotometric data (using Excel or the graph paper below), labeling the four stages of the growth curve- lag, log (exponential), stationary, death. Be sure to label your axes properly and give your graph a title Time Culture Sampled                        % Absorbance 0 min                                                       5% 30min                                                              7% 60min                                                              9% 90min                                                              15% 120min                                                            25% 150min                                                            37% 180min                                                            49% 210min                                                            50% 240min                                                            49% 270min…

Ten grams of hamburger were added to 90 mL of sterile buffer. This was mixed well in a blender. One-tenth of amL of this slurry was added to 9.9 mL of sterile buffer. After thorough mixing, this suspension was further dilutedby successive 1/100 and 1/10 dilutions. One-tenth of a mL of this final solution was plated onto Plate Count agar.After incubation 145 colonies were present. How many colony-forming units were present in the total 10 gramsample of hamburger?