Q: Illustrate the step by step method of preparing culture media.
A: Introduction Culture medium:- It is a liquid or gel designed for specific mixtures of nutrients and…
Q: Experiment :method of sterilization on glassware and media
A: Sterilization is a process by which all forms of life are killed, deactivated or removed from a…
Q: What is the purpose of flame-sterilizing the mouth of the test tube and the side of the Petri dish…
A: Flame is an excellent way of cleaning a surface from contaminating organisms. Instant high heat…
Q: Why don't you get isolated colonies from the pour plate if the broth culture was not diluted first?
A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…
Q: Why does a streaking method used to inoculate plates result in isolated colonies?
A: Streaking method is used to obtain pure colonies of microbes(bacteria) from a proper single species…
Q: in the clinic, the acid-fast stain is used to diagnose infection with which bacterium?
A: The Ziehl-Neelson (ZN) stain is the most commonly utilized of the different types of AF staining…
Q: Why is the culture medium referred to as a highly enriched laboratory soil?
A: Culture medium is referred to as highly enriched laboratory soli because it provides all the…
Q: Why was a blood agar, rather than a nutrient agar, plate used for the culture from your mouth?
A: Cultured bacteria are any bacteria that grow in the lab by the microbiologist using the required or…
Q: What is the difference between the pour plate method and the spread plate method? Why might you…
A: Standard plate count method is a growth based approach that counts the number of living…
Q: Why is there a need to use a staining agent in observing the specimen?
A: Staining agent: The things that owes-out stains were recognized as staining agent,…
Q: Why would the optical tweezers be a method superior to dilution and liquid enrichment for obtaining…
A: Optical tweezers have been used to trap dielectric spheres, viruses, bacteria, living cells,…
Q: How does bacteria grow on agar plates?
A: Bacteria are the most primitive organisms on Earth. They can survive in most extreme environments as…
Q: What are pure cultures and why are they important? How are spread plates, streak plates, and pour…
A: In science and related fields, a culture plate is a low level lined lab holder for growing a layer…
Q: If any of the water sample plates had a lawn or too many colonies to count, what might you have…
A: A technique known as the standard plate count is used for estimation of the population density in…
Q: What are the 4 types of culture?
A: Biological cultures include common laboratory means, which are involved in studying living organisms…
Q: hy do we use broth for enrichment, but plates for isolation and identification?
A: What do you think of when you hear the word broth? Maybe it is a delicious liquid made from chicken,…
Q: What do to do when the disstillation did not make the sample completely pure? What could have went…
A: Distillation is a process that is based upon the boiling points of various liquids present in the…
Q: What would happen if you had skipped the heat fixing step and continued with the staining procedure?
A: There are various staining methods are used for staining the bacteria, namely simple staining,…
Q: What are the purposes of carrying out the streak plate andpour plate techniques in microbiology?
A: It is a technique used to isolate a pure strain from a single species of microorganism, often…
Q: What are the factors which need to be considered in the selection of culture vessel?
A: Culture vessels:- Culture vessels provide a contamination barrier to protect the cultures from the…
Q: How is the correct way of incubating culture plate media? Explain why is that so.
A: After the way of life medium is set, and streaked with the necessary microbe/stock, the cover is put…
Q: How is agar in the petri dish prepared to grow the bacterial specimen? explain in your own words.
A: Agar is a polymer made up of subunits of sugar galactose and is a component of cell walls of several…
Q: Explain why it is important to use only a small amount of bacteria when preparing a smear
A:
Q: WHAT IS THE TYPE OF Growth patterns on Agar Slant and Broth Medium
A: Growth and morphogenesis of plant is determined by the composition of culture media. There are few…
Q: What is the media used in Urease Test and expected results?
A: Urease test is defined as a biochemical test that distinguishes the basic alkaline fermentation of…
Q: What is the name of this media? What type of media is it? What does the yellow color indicate? What…
A: Selective media generally selects for the growth of a desired organism, stopping the growth of or…
Q: What is the main advantage of using the slide culture technique for identifying molds?
A: Side Culture - It is a rapid method of preparing fungal colonies for examination and identification.…
Q: Why is it necessary to perform a plate count in conjunction with the turbidimetry procedure?
A: Plate counting is one of the most fundamental techniques used in microbiology. It is used to…
Q: Why does dry heat require higher temperatures for longer time periods to sterilize than moist heat
A: Sterilization may be defined as the process which help in the complete removal of all…
Q: Distinguish batch culture and continuous culture
A: Fermentation is used to convert carbohydrates into alcohol or acids. This process occurs in some…
Q: Of what practical importance are air borne microorganisms to the laboratory workers? What…
A:
Q: What is the best streaking technique in a culture media? Explain
A: In microbiology, there are five I's that symbolise five different procedures used in the laboratory…
Q: Which of the following control agents help to be used to achieve sterility? - Virucide - Sporocide…
A: Sterilization can be achieved through various means, including heat, chemicals, irradiation, high…
Q: Based on the growth you saw on your Lee’s Agar plate, what conclusions can you make about the…
A: Lee Agar is used to study the bacteria that can grow on yogurt. It is primarily used to count the…
Q: What type of agar is MacConkey agar?
A: MacConkey agar or MAC which is also described as a bacterial culture medium was named after the…
Q: Why should you filter some liquids for sterilization, rather than autoclaving them?
A: To control microbial growth or contamination there are a number of aseptic techniques developed that…
Q: Which culturing method provided the clearest results, the semisolid medium tube or the soft agar…
A: Agar is a jelly-like substance obtained from red algae. It is a mixture of two components namely…
Q: explain methods for cleaning beans using flat bed screen and drum spray cleaner
A: Much of the mechanical cleaning procedures must be performed in the field for proper storage of the…
Q: Turbidometry is a method used for counting microbes?
A: Microbiology is the branch of biology that deals with microorganisms. Bacterial cultures and their…
Q: What is the hazard of the spattering tendency in heating
A: Sterilization is the process of cleaning a substance by removing, killing or deactivating all forms…
Q: What are the immersion media that need to be used?
A: Immersion media is used to increase the numerical aperture of the microscope by a factor equal to…
Q: What method can be used to achieve sterilization?
A: It is required to describe the method to be used to achieve sterilization.
Q: explain why you would expect to see similarities in the types of colonies on the fomite plates and…
A: Organisms that are too small to be seen by the naked eye are microorganisms. These organisms can be…
Q: What are the primary uses of the following preparations of culture media? Agar regular slant Agar…
A: Hello. Since your question has multiple sub-parts, we will solve the first three sub-parts for you.…
Q: What is the preparation condition for culturing a mould sample?
A: Introduction: Mould is a fungal specimen which is grown in filamentous structure called hyphae. They…
Q: What are the different types of Pasteurization?
A: Pasteurization is the process of heat processing the liquid (mainly milk) or food so that the…
Q: What is this media? What type of media is it? Which type of organism can grow on this plate?…
A: Media is... Agar This is the solids type of Media. Organism type... Streak plate technique is used…
What is the nature of samples that should NOT be placed inside the drying-oven?
Step by step
Solved in 2 steps
- What is the difference between a mixed culture and a contaminated culture if they both contain more than one type of organism ?In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?
- Why is dilution a necessary part of pure culture preparation?How to prepare an agar plate for bacteria? How to include the antibiotics into agar plates? How long should you wait for bacterial colonies to grow on agar plates? What is the appropriate temperature?.What is a mixed culture? A contaminated culture?
- What is the difference between the pour plate method and the spread plate method? Why might you choose one over the other? What kind of plate must this be and why do you say that?In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable to use refrigerated samples for this procedure Why or Why not?What do to do when the disstillation did not make the sample completely pure? What could have went wrong?
- You are hired in one of the microbiology labs in Memphis and your first assignment to identify microbial samples from a local restaurant. You are given a set of slides and asked to classify the specimens as Gram positive or Gram negative. 6) What would be the last reagent you use to identify the bacteria? 7) What type of visual aid you need to help you see the bacterial samples clearly? 8) After the use of what reagent, you will be able to decide if you have a Gram positive or Gram negative bacteria? **Please give the answer**Why do you think the continuous culture has higher chances of contamination than batch and fed-batch culture?Why sometimes specimen may show organisms under the microscope but not appear in the culture media?