Q: What types of samples can be measured with Circular Dichroism? Can information be extracted?
A: Circulate dichroism is the difference in the absorption of left handed circularly polarised…
Q: What is the function of the mordant used in the Gram staining procedure? If the iodine step is…
A: Gram stain or Gram staining, likewise called Gram's strategy, is a technique for staining used to…
Q: What advantages do solid media offer for the isolation ofmicroorganisms?
A: Micro-organism are those that cannot be seen by the naked eye, but are visible under microscopes.…
Q: Why don't you get isolated colonies from the pour plate if the broth culture was not diluted first?
A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…
Q: How to prepare tryptone agar, tryptone broth and tryptone soft agar from other chemical contents and…
A: Tryptone is formed by the enzymatic digestion of casein by the trypsin enzyme. Tryptone is used as a…
Q: Describe how the streak-plate method is used to obtain a pure culture and how the resulting culture…
A: In microbiology, culture refers to microbial growth of a certain species maintained in controlled…
Q: What is agar and why is it necessary for growing bacteria on plates?
A: Agar is a mixture of two polysaccharides- agarose and agaropectin. It is derived from red-purple…
Q: In gram staining, could colors other than red be used as a counterstain?
A: A counterstain is a stain with color contrasting to the principle stain, making the stained…
Q: Why is it sufficient to stain cells from a SDA (Sabouraud Dextrose Agar) plate with crystal violet…
A: Introduction : Sabouraud agar, often known as Sabouraud dextrose agar (SDA), is a peptone-containing…
Q: Discuss the most important thing to remember in preparing and inoculating culture media?
A: Introduction: Microbes are a source of contamination and infection. They are categorized as…
Q: What is the difference between the pour plate method and the spread plate method? Why might you…
A: Standard plate count method is a growth based approach that counts the number of living…
Q: What is a pure culture?
A: Introduction Culture is referred to th process of growing the cell outside the animal or plant body…
Q: Serial dilution is recommended prior to pour and spread plate. Why is this so?
A: Pour Plate: it is a technique in which A plate made by combining the inoculum which is cooled but…
Q: If you transfer 0.1 mL of culture into a 99mL of sterile water, then add 1mL of that to an agar…
A: Making a solution thinner or less dense is the procedure of dilution. Serial dilutions (also known…
Q: Why is it important to use a sterilized loop between streaks when preparinga streakplate?
A: Streaking is a technique used to isolate a pure strain from a single species of microorganism, often…
Q: When it comes to simple stains, why is time so important? And what is the purpose of rinsing the…
A: Microbiology is a hugely important field in both basic and applied research. New and fascinating…
Q: What are the applications for Pasteurization, Autoclaving, UV irradiation, and Gamma irradiation?
A: It is required to mention the application of Pasteurization, Autoclaving, UV irradiation, and Gamma…
Q: What advantage(s) does the pour plate method have over the streak plate method?
A: In microbiology. streaking is a technique for the isolation of the pure strain from the population.…
Q: What is the difference between streak plate method and spread plate technique?
A: Answer of the question given below...
Q: During the preparation of the capsule stain, the slide used to spread the India ink/bacteria mixture…
A: Capsule stain is a type of differential stain which uses acidic and basic dyes to stain the…
Q: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
A: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
Q: What is the purpose of food irradiation, and how does it work?
A: Irradiating food kills bacteria and extends life of the food.
Q: What are the purposes of carrying out the streak plate andpour plate techniques in microbiology?
A: It is a technique used to isolate a pure strain from a single species of microorganism, often…
Q: How is the correct way of incubating culture plate media? Explain why is that so.
A: After the way of life medium is set, and streaked with the necessary microbe/stock, the cover is put…
Q: In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable…
A: Harada-Mori culture technique It is a method of incubating fecal material on a filter paper strip in…
Q: suffering severely due to alkaloid production and accumulation in the medium the
A: Plants have an important source of life-saving medications where it is used in two ways such as…
Q: What is one advantage of a pour plate over a streak plate?
A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…
Q: When labeling culture tubes or plates why do we use a pencil instead of a pen? Are there other…
A: Appropriately labeling specimens for research labs is of critical importance.
Q: What are the advantages and disadvantages of using the Wet Mount technique?
A: As per bartleby guidelines, we are suppose to answer only one question kindly post other separately.…
Q: For preparation of a smear on a slide, what is the purpose of heat fixation? What problems can arise…
A: The preparing a slide that has to be observed under a microscope there are multiple steps that are…
Q: What should be considered when preparing a monolayer cell culture?
A: Monolayer culture, also known as adherent culture, is a form of animal cell culture in which cells…
Q: How do Blood agar & EMB agar help us to identify organisms more specifically than a gram stain?
A: How do Blood agar & EMB agar help us to identify organisms more specifically than a gram stain?
Q: Distinguish batch culture and continuous culture
A: Fermentation is used to convert carbohydrates into alcohol or acids. This process occurs in some…
Q: How does an autoclave sterilize instruments? What is used to dry glassware and at the same time
A: Autoclaving is the best method for sterilizing glass instruments. Sterilizing means removal of all…
Q: Which stains are differential in microbiology?
A: In dis a staining process in which we use more than one chemical stain to differentiate between…
Q: What is the nature of samples that should NOT be placed inside the drying-oven?
A: According to the question, we have to explain the nature of the samples that should not be placed…
Q: In Gram staining method, list the reagents and state their role for each step of the procedure? In…
A: Bacteria are unicellular, prokaryotic organicism which are devoid of membrane bound organelles and a…
Q: To measure the culture temperature, why is the thermometer placed in a tube separate from the…
A: A microbe or microorganism is a microscopic organism that is too small to be seen with the naked…
Q: What is the best streaking technique in a culture media? Explain
A: In microbiology, there are five I's that symbolise five different procedures used in the laboratory…
Q: What is the function of a mordant, and which reagent serves this purpose in the Gram stain…
A: An extensively used microbiological method of staining is called a Gram stain. It significantly…
Q: How does the age of a culture affect the Gram stain reaction? What is an optimum culture age for a…
A: To identify the unknown bacteria this gram staining is used. This staining technique differentiate…
Q: How to prepare an agar plate for bacteria? How to include the antibiotics into agar plates? How long…
A:
Q: What are the primary uses of the following preparations of culture media? Agar regular slant Agar…
A: Hello. Since your question has multiple sub-parts, we will solve the first three sub-parts for you.…
Q: What is the difference between simple and differential stains? Give an example of a differential…
A: Bacteria are microorganisms that are colorless for most of the part and in order to study their…
Q: What are the different types of Pasteurization?
A: Pasteurization is the process of heat processing the liquid (mainly milk) or food so that the…
Q: What is this media? What type of media is it? Which type of organism can grow on this plate?…
A: Media is... Agar This is the solids type of Media. Organism type... Streak plate technique is used…
Q: How would you inoculate a plate to get a 1:10 dilution? A 1:100 dilution?
A: Inoculation is defined as the process of introduction of microorganisms into a culture for their…
Q: Dilute cells from an old culture 1:50 into 200 mL cultures. What volume of the old culture would you…
A: Culture consists of appropriate substances which provides the growth to the desired organisms.
What are pure cultures and why are they important? How are spread plates, streak plates, and pour plates prepared?
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- What is one advantage of a pour plate over a streak plate?What is the major difference between Thayer-Martin and chocolate agar? When would you use Thayer-Martin rather than chocolate agar?What is the difference between the pour plate method and the spread plate method? Why might you choose one over the other? What kind of plate must this be and why do you say that?
- In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable to use refrigerated samples for this procedure Why or Why not?What is the best streaking technique in a culture media? ExplainWhat is the goal of isolation streak plate technique? Why is microbial considered a pure culture?