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Of what practical importance are air borne microorganisms to the laboratory workers?  What precautions should be taken to control laboratory contaminants?

Of what practical importance are air borne microorganisms to the laboratory workers?  What precautions should be taken to control laboratory contaminants?

Principles Of Pharmacology Med Assist
Principles Of Pharmacology Med Assist
6th Edition
ISBN:9781337512442
Author:RICE
Publisher:RICE
Chapter12: Parenteral Equipment And Supplies
Section: Chapter Questions
Problem 22RQ
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Of what practical importance are air borne microorganisms to the laboratory workers? 

What precautions should be taken to control laboratory contaminants? 

Of what advantage is the using a solid and a liquid medium?  

MICROORGANISMS IN THE ENVIRONMENT II. Materials O 7 sterile petri dishes Sterile nutrient agar O Water bath III. Procedures: 1. Melt nutrient agar in a water bath and cool to approximately 45-degree Celcius. 2. Into each sterile petri dish, pour about 15-20 ml nutrient agar following aseptic technique. 3. Upon solidification of the medium, label dishes A, B, C, D, E, F, & G. A. Remove cover and swab a tv remote control then rub it over the surface of the agar,then cover the plate. B. Remove cover and cough directly into the agar surface 2 to 3 times then cover the plate. C. Remove cover and rub tips of your fingers over the surface of the agar then cover the plate. D. Remove cover and swab the sole of the shoe and rub tips over the surface of the agar, then cover the plate. E. Pick up a small amount of dust on a piece of cotton and rub over the agar surface, cover the plate. F. Open the petri dish and pass it over the hair, then cover. G. Leave this plate untouched. 4. Incubate the plates in an inverted position at 37-degree Celcius. Make your observation after three days.
Transcribed Image Text:MICROORGANISMS IN THE ENVIRONMENT II. Materials O 7 sterile petri dishes Sterile nutrient agar O Water bath III. Procedures: 1. Melt nutrient agar in a water bath and cool to approximately 45-degree Celcius. 2. Into each sterile petri dish, pour about 15-20 ml nutrient agar following aseptic technique. 3. Upon solidification of the medium, label dishes A, B, C, D, E, F, & G. A. Remove cover and swab a tv remote control then rub it over the surface of the agar,then cover the plate. B. Remove cover and cough directly into the agar surface 2 to 3 times then cover the plate. C. Remove cover and rub tips of your fingers over the surface of the agar then cover the plate. D. Remove cover and swab the sole of the shoe and rub tips over the surface of the agar, then cover the plate. E. Pick up a small amount of dust on a piece of cotton and rub over the agar surface, cover the plate. F. Open the petri dish and pass it over the hair, then cover. G. Leave this plate untouched. 4. Incubate the plates in an inverted position at 37-degree Celcius. Make your observation after three days.
HOW DO I DISPOSE OF THE PLATES? It is important you do not place your plates in the trash without first taking some precautions. While bacteria in small numbers may be harmless, once cultured into millions of cells they can pose a greater threat. There are a few ways to properly dispose of your used agar plates, depending on what you have or available. 1.lf you have an autoclave, you can autoclave your plates per the directions on your autoclave (generally at least 30 minutes). 2. If you used glass petri dishes and don't have an autoclave, prepare a 20% bleach solution, and spray your plates down. Allow the bleach to soak into the agar for 1 hour before placing agar in the trash. Then thoroughly clean your empty petri dishes again with a bleach solution and distilled water rinse. 3. If you used plastic petri dishes that you can afford to part with, you can place them in bio-hazard bags and have your district arrange for biohazard pick up. It doesn't hurt to spray them down with a 20% bleach solution before placing them in bio-hazard bags
Transcribed Image Text:HOW DO I DISPOSE OF THE PLATES? It is important you do not place your plates in the trash without first taking some precautions. While bacteria in small numbers may be harmless, once cultured into millions of cells they can pose a greater threat. There are a few ways to properly dispose of your used agar plates, depending on what you have or available. 1.lf you have an autoclave, you can autoclave your plates per the directions on your autoclave (generally at least 30 minutes). 2. If you used glass petri dishes and don't have an autoclave, prepare a 20% bleach solution, and spray your plates down. Allow the bleach to soak into the agar for 1 hour before placing agar in the trash. Then thoroughly clean your empty petri dishes again with a bleach solution and distilled water rinse. 3. If you used plastic petri dishes that you can afford to part with, you can place them in bio-hazard bags and have your district arrange for biohazard pick up. It doesn't hurt to spray them down with a 20% bleach solution before placing them in bio-hazard bags
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