vs. size Interpret the results shown on the graph below which shows b generation ibition zone in 3 treatments. Describe what is happening in terms of variation, fitness, & selection. Mean Width of Inhibition Zone (mm) 202 XXII 1 2 Triclosan -Ethanol --- Water 3 4 Round of Testing Describe this graph. What can you say about: 1. Variation? 2. Fitness? 3. Selection? Mean widths of zones of inhibition. Treatments were water, 17.5% ethanol, and triclosan (500μµgl). Round of testing refers to the number of exposures to treatment. Error bars encompass 95% confidence intervals on each mean.
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- esting 8MU376MpwkNtkrF5%252B26BVPP%252Bzg%253D%2. Question Image 1 3 6. 7. 8. with the figure? cracteristics ight differe dividuals can compared Q. Which of following can you do with the biotechnology depicted in the figure? • 0 1:47 Sign out || O|| | 111. There are many ways you can accomplish a 1000-fold dilution. Propose 3 different serial dilution methods that will accomplish a dilution with a 1:1000 dilution factor. 2. Suppose you count 35 bacterial cells from a solution that has gone through the following serial dilutions: two 10-fold dilutions, followed by a 5-fold dilution, followed by 2-fold dilution. What should be the bacterial count from the original solution? Show your work! 3. When making a dilution you need to put different volumes of the stock solution and the buffer. Suppose you are doing a 2-fold dilution, where both volumes are the same. Will you use the same pipette tip to extract the stock and the buffer solution? Explain your reasoning.1. What is the generation time of a certain bacteria with an initial number of bacterial cells of 1x104 increases to 1x10¹5 after 5 hours and 25 minutes? 2. How long would it take for a certain bacterial cell to increase from 1x10² to 1x10²7 when the generation time 25 min?
- You seed another culture of different end cell line, Walker12 cells, at 8 am on Friday with 10^4 cells. By Thursday at noon (12:00 pm) they reach confluence. Assume the sae confluency as stated above for a T150 flask (culture area of 150cm^2 adn when confluent contains 2 x 10^7 cells). What is the generatoin time (doubling time)? A. 120 hours B. 80 hours C. 38 hours D. 8 hours E. 11.4 hours F. other ________Im 100 4. You have a culture of yeast that is at a density of 8x10' cells/mL. You dilute the sample 1:10, then 1:1,000 again, and finally you dilute the sample an additional 1:5. You add 0.1 mL of the final dilution to a spread plate. Assuming that most of the cells in the original culture were living, how many colony- forming units do you expect to count on your pour plate the next day?Five different HFR strains were isolated from a single F+ strain of E. coli. Timed matings showed that they transferred the following loci during the first hour of mating. The earliest loci are on the left, the slowest on the right for each order. Map these loci to a circle. strain early →→late 1. QZ ARL What markers are between B and X (shortest route)? SNWT O EMQ 3. 5. RBLE XAR BEMQZ EBLRA ZSNWT strain early →late 2. 4. TXARL XTWNS
- 1. If a negative control produces a band, what does this indicate? 2. In an experiment, a student’s sample amplified for D1S80 produced 3 bands. It was the only DNA sample run on the gel. The student knows that there was no problem with the Thermocycler or primers because the other students in the class had the expected results of only one or two bands. What is the most likely explanation for these results?Match each of the descriptions in Column A with the correct panel listed in Column B. Description Panel Resistant bacteria share resistance genes with each other Resistant bacteria survive and reproduce Treatment with methicillin kills nonresistant bacteria Some bacteria carry a mutation that provided resistance to methicillin Predict the possible outcome(s) if the antibiotic tetracycline was applied after panel 4. Check all that apply. O The tetracycline will act as a selective pressure, causing bacteria to mutate in order to survive. O Since the population is resistant to methicillin and not tetracycline, none of the bacteria will survive. O Treatment with an antibiotic will not affect the overall survival of the population. Any bacterium that has a mutation for tetracycline resistance would be able to survive.4) Consider the following list of hypothetical antibiotics, tested against Escherichia coli in a Kirby-Bauer test: Antibiotic Zone of Inhibition (mm) Astonostatin 12 Bodaciosporin 25 Crazifloxacin 5 Dorkimycin 16 When the above antibiotics were tested as treatments for E. coli infections in humans, it was found that Dorkimycin has virtually no effect on the infection, while Astonostatin is the most effective of the four antibiotics listed. Propose an explanation for this result. Based purely on these results, can you draw up a similar table for predicted zones f inhibition for Staphylococcus aureus? Why or why not?
- A B Which of the bacterial colonies shown (A or B) was nonlethal when injected in mice? А ВHi, I am working on qPCR quantification for specific bacteria. However, when the CT value achieve greater than 30, it always shown high CT value differences in my duplication no matter I repeat the same set of experiment for those samples with low quality. My question is, what makes this happening? The other question is, how many CT value differences is acceptable for duplication?ery t ess Which method of counting cells would you use for each of these situations? Viable Cell Count Optical Density measurement Direct cell count [Choose ] [Choose ] ✓ [Choose ] You are attempting to quantify the ability of cleaning agents to kill bacteria in a liquid culture You are attempting to determine the doubling time of Escherichia coli in various liquid media You are attempting to quantify the ratio of contaminating rod-shaped cells in your culture of spherical Staphylococcu