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Basic Clinical Laboratory Techniques 6E

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter6: Basic Clinical Chemistry

Section6.8: Fecal Occult Blood Test

Problem 10RQ

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Need help figuring out the top two CFU questions. Unsure of how to calculate

Using the count data and observational data you acquired, calculate the number of CFUs in the original undiluted sample.
CFUS
Number of CFUS =
You discover that the plate you selected had only been inoculated with 0.1mL of the dilution instead of 1mL. Using the count data and observational data you acquired,
re-calculate the number of CFUs in the original undiluted sample.
Lab Data
Number of CFUs =
CFUS
Why is it necessary to dilute a sample in order to determine bacteri
O A. Dilution ensures that there are enough nutrients in the medium for gro
O B. The dilution process encourages the growth of more and different type
C. The bacterial density in the original sample may be too high for the for
O D. The dilution process is conducted to save time when counting.
While moonlighting in the hospital lab, you receive an order for a ra
based on a quantitative urine culture yielding greater than 100,000
come back from incubation the next day, but many of your plates w
you to count. It yields 102 isolated colonies from a 0.1 mL inoculur
METHODS
Phase 1
Plate
#
1
2
3
4
Image
Dilution Label
1:10,000
1:100,000
1:1,000,000
1:10,000,000
Phase 2
Growth Appearance on Plate
Heavy
Heavy
Intermediate
Scant
Plate 1
Sample Dilution
Colony Count
You Can
Plate Selected For Counting
Volume of Diluted Sample (mL)
DA How to Cala
Plate 2
No
Isolated Colonies
Present?
Yes, few
Yes, many
Yes, few
000,cogli
Plate 3
Countable?
Too numerous to count
Too numerous to count
Countable
Too few to count
000,000,01:1
Plate 4
Plate 3
1
1:1,000,000
46
- X

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Calculate the concentration of bacteriophage in the original culture from the following data. Be sure to include units. Show dilution factors for each test tube. Show the final dilution factor for test tube number 4. Show all your math. 0.1 ml 0.01 ml 0.001 ml 0.1 ml 1.0 ml plated 9.9 ml 9.99 ml 9.999 mi 9.9 m plaques Original Culture Test Test Test Test Tube #1 Tube #2 Tube #3 Tube #4 Dilution Factor: Concentration of Phage:
At the station there is a 2 mL microcentrifuge tube containing sheep blood and 3 tubes containing the following solutions: 0 mM, 300 mM, 600 mM of sucrose. The tubes are randomly labelled A, B, C. 2 mL of each solution were transferred to a different microcentrifuge tube, 20 ul of blood were then added to each tube, and they were mixed well by inverting the tube for multiple times. They were observed and it was determined whether the solution in each tube was hypotonic (Yes or No) relative to the blood cells. In addition, the solutions were diluted with water to help you determine which solution is which sucrose concentration. What sucrose concentration corresponds with each tube? Tube Hypotonic (Yes/No) Hypotonic after 1:1 dilution with water (Yes/No) Most likely Sucrose concentration (mM) A No Yes B Yes Yes C No No
Below is a set of data for a serial dilution/plate count experiment. Based on this data what is the calculated concentration of bacteria in CFU/mL in the original culture? The OD600 of a bacterial culture that's been diluted in half is 0.842. What is the estimated population of cells in the original undiluted culture? asap
The following are errors that people commonly make when they perform serial dilutions. Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you make this mistake. You intend to add 0.9 ml of diluent to each tube and 0.1 ml of culture. Instead, you add 0.5 ml of diluent to each tube and 0.1 ml of culture to the first tube. Then, you make a serial dilution of 0.1 ml into and from each tube as described. You prepare 0.9 ml of diluents in each tube. You add 0.1 ml of culture (from the overnight culture provided) to every tube. You add 0.9 ml of diluent to each tube. You add 0.1 ml of culture to the first tube and mix. You get distracted, and transfer 0.1 ml to the third tube instead of the second. You perform the rest of the series as described.
a. What is the total dilution of Tube #4? Express the answer in exponential format. b. You plated 1 mL of the Tube #4, After incubating, you counted 500 colonies on the plate. What is the concentration of Tube #0? include units. c. How could you change the experiment in part B to get a plate in the countable range? Be specific about any dilution factors and/or plated volumes you would change.
Why is a 1:20 dilution of patient serum, rather than undiluted patient serum, used for the qualitative test?
With a plate count average of 297 and a final dilution on the plate of 1:1,000 ; what is the viable number of cells in original culture (CFU/ml)?
A culture is incubated for 10 hours. 1 hours after inoculation it reached the exponential growth phase. At this time point the cell density was 1x10^4 cells/ml. 5 hours after inoculation (still during the exponential growth phase) the cell density was 1x10^7 cells. Calculate K and g(t). The growth constant (k) is minute. (round to 3 decimal points) The generation time (gt) is minutes. (round to whole number)
A sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it. Assuming you plated the same volume on each plate, how many colonies would you expect to find on the 10^-2 plate? Include units and use OCD formula.
What is the purpose of the RPR (RAPID PLASMA REAGIN ) Test? Why is a qualitative test performed before a semi-quantitative test? Why is it necessary to rotate the slide/card?
You were instructed to add 1.0ml out of 4.0ml of an undiluted sample to 99ml of sterile diligent you add the entire 4.0ml to 99 ml. a) what was your intended dilution factor? b) what was your actual dilution factor?
Determine what percentage of the culture was living (viable) and what percentage was dead (mortality). Plates Plate Dilution Volume plated No.of colonies Avg No Concentration of diluted sample Cd(cells/mL) Concentration of original sample Cu(cells/mL) 1 10^-3 10ul R1=130,R2= 110,R3=210 150 150mL 1.50*10^6 Volume of cells(mL) Volume of diluent(mL) Total dilution(D) Hemocytometer count Avg cells in 1 mm^2 area Concentration of diluted sample Cd(cells/mL) Concentration of original sample Cu(cells/mL) 4.3 0.5 0.1 grid 1= 171 , grid2 = 185 178 1.78*10^5 1.78*10^6

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