Aquaculture Science
3rd Edition
ISBN: 9781133558347
Author: Parker
Publisher: Cengage
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- Imagine you have been given a liquid culture of yeast with a starting concentration of 3.67 x 10' cells/ml and are asked to carry out the sample dilution process shown in the figure below. 100μl 100μl 100μl 100μl 100μl 0.9ml 0.9ml 0.9ml H2O H₂O 6.9ml 0.9ml H₂O H₂O H₂O Original 10-1 102 10-3 104 Culture 105 100μl 100μl 100μl Plate A Plate B Plate C a. How many colonies should have been present on Plate A in this example? - Answers must be whole numbers as partial colonies are not expected. b. Imagine you carried out the same dilution scheme shown in the figure above, but now, you do not know the concentration of the original culture. If you counted 163 colonies on Plate B, what is the concentration of cells/ml in the original culture?arrow_forwardCould you please assist with correct number?arrow_forwardYou have a starter culture containing 8 x 109 cells per mL, from which you take 10mL to inoculate a fresh 1 L culture. After 15 hours, the cell density of the new culture is 3 x 1012cells per mL. What are the generation time and the mean growth rate constant of theorganism in culture?arrow_forward
- As shown in this diagram, you perform a ten-fold serial dilution of a culture to determine the number of colony forming units (CFU) per mL it contains. You do a plate count with these growth results (no. of colonies for each dilution): 1:10 too many to count; 1:100 too many to count; 1:1,000 174; 1:10,000 23; 1:100,000 no growth. The number of CFU per mL in the original culture was: 1 ml 1 ml Original inoculum Dilutions 9 ml broth in each tube 1:10 1 ml 174,000 1:100 1 ml 1 ml 1 ml 1:1000 1 ml 1:10,000 1 ml None of the other four answers (Correct answer not given) 1,000 230,000 174 1 ml 1:100,000 1 mlarrow_forwardAs shown in this diagram, you perform a ten-fold serial dilution of a culture to determine the number of colony forming units (CFU) per mL it contains. You do a plate count with these growth results (no. of colonies for each dilution): 1:10 too many to count; 1:100 too many to count; 1:1,000 312; 1:10,000 38; 1:100,000 no growth. The number of CFU per mL in the original culture was: A. 38 B. 380,000 C. 38,000 D. None of the other four answers (Correct answer not given) E. 10,000arrow_forwardery t ess Which method of counting cells would you use for each of these situations? Viable Cell Count Optical Density measurement Direct cell count [Choose ] [Choose ] ✓ [Choose ] You are attempting to quantify the ability of cleaning agents to kill bacteria in a liquid culture You are attempting to determine the doubling time of Escherichia coli in various liquid media You are attempting to quantify the ratio of contaminating rod-shaped cells in your culture of spherical Staphylococcuarrow_forward
- 3. Please help answer this question and please show all work on how you got the answers. Thank you so much!! :)arrow_forwardYou perform a ten-fold serial dilution of a bacterial culture to determine the number of colony forming units (CFU) per mL in the culture. You then do a plate count with these growth results (no. of colonies): 1:10: too many to count; 1:100 too many to count; 1:1,000: 126 colonies; 1:10,000 14 colonies; 1:100,000: no growth The number of CFU per mL in the original culture was: 1 ml 1 ml 1 ml 1 ml Original inoculum Dilutions 1,000 126 9 ml broth in each tube 126,000 10,000 140,000 1:10 1 ml 1:100 1 ml 1:1000 1 ml 1 ml 1:10,000 1 ml 1:100,000 1 mlarrow_forwardA serial dilution of a bacterial culture yields the following number of colonies. Which plate(s) should be used to determine the original cell density? Plate A Plate B Plate C Plate D Plate E Dilution Factor 10-5 10-6 10-7 10-8 10-9 # of Colonies Too many to count 850 456 80 14 Group of answer choices All of these choices A & B D & E E None of these choices A B & C D B C & D Carrow_forward
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