4. You have a culture of yeast that is at a density of 8x107 cells/mL. You dilute the sample 1:10, then 1:1,000 again, and finally you dilute the sample an additional 1:5. You add0.1 mL of the final dilution to a spread plate. Assuming that most of the cells in the original culture were living, how many colony- forming units do you expect to count on your pour plate the next day?

4. You have a culture of yeast that is at a density of 8x107 cells/mL. You dilute the sample 1:10, then 1:1,000 again, and finally you dilute the sample an additional 1:5. You add0.1 mL of the final dilution to a spread plate. Assuming that most of the cells in the original culture were living, how many colony- forming units do you expect to count on your pour plate the next day?

Aquaculture Science

3rd Edition

ISBN:9781133558347

Author:Parker

Publisher:Parker

Chapter2: Aquatic Plants And Animals

Section: Chapter Questions

Problem 2KA

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4. You have a culture of yeast that is at a density of 8x107 cells/mL. You dilute the sample 1:10, then 1:1,000 again, and finally you dilute the sample an additional 1:5. You add 0.1 mL of the final dilution to a spread plate. Assuming that most of the cells in the original culture were living, how many colony- forming units do you expect to count on your pour plate the next day?
Imagine you have been given a liquid culture of yeast with a starting concentration of 3.67 x 10' cells/ml and are asked to carry out the sample dilution process shown in the figure below. 100μl 100μl 100μl 100μl 100μl 0.9ml 0.9ml 0.9ml H2O H₂O 6.9ml 0.9ml H₂O H₂O H₂O Original 10-1 102 10-3 104 Culture 105 100μl 100μl 100μl Plate A Plate B Plate C a. How many colonies should have been present on Plate A in this example? - Answers must be whole numbers as partial colonies are not expected. b. Imagine you carried out the same dilution scheme shown in the figure above, but now, you do not know the concentration of the original culture. If you counted 163 colonies on Plate B, what is the concentration of cells/ml in the original culture?
The following image shows part of counting chamber you used to estimate cell viability for yeast cells. If the dilution factor you used was 1:200, and the total sample volume was 5 ml, then calculate the cell viability, the cell density and the total cell.
Could you please assist with correct number?
You have a starter culture containing 8 x 109 cells per mL, from which you take 10mL to inoculate a fresh 1 L culture. After 15 hours, the cell density of the new culture is 3 x 1012cells per mL. What are the generation time and the mean growth rate constant of theorganism in culture?
As shown in this diagram, you perform a ten-fold serial dilution of a culture to determine the number of colony forming units (CFU) per mL it contains. You do a plate count with these growth results (no. of colonies for each dilution): 1:10 too many to count; 1:100 too many to count; 1:1,000 174; 1:10,000 23; 1:100,000 no growth. The number of CFU per mL in the original culture was: 1 ml 1 ml Original inoculum Dilutions 9 ml broth in each tube 1:10 1 ml 174,000 1:100 1 ml 1 ml 1 ml 1:1000 1 ml 1:10,000 1 ml None of the other four answers (Correct answer not given) 1,000 230,000 174 1 ml 1:100,000 1 ml
As shown in this diagram, you perform a ten-fold serial dilution of a culture to determine the number of colony forming units (CFU) per mL it contains. You do a plate count with these growth results (no. of colonies for each dilution): 1:10 too many to count; 1:100 too many to count; 1:1,000 312; 1:10,000 38; 1:100,000 no growth. The number of CFU per mL in the original culture was: A. 38 B. 380,000 C. 38,000 D. None of the other four answers (Correct answer not given) E. 10,000
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You have spread 0.1ml of a 1x10-8 diluted bacterial culture sample on a Petridish and counted35 colonies. What was the cell density of your original culture (in cells/ml)? How many cellsdid you have in 100ml of that culture? DON’T FORGET TO ROUND!
3. Please help answer this question and please show all work on how you got the answers. Thank you so much!! :)
Below is the colony diameter for each yeast culture grown on PDA and AcA incubated at 35°C measured at Day 7. From the data presented above, what could you observe about the growth of the yeast cultures on PDA and AcA? Cite possible reasons behind this.
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