VISUAL SKILLS Griffith was trying to develop a vaccinefor S. pneumonia when he was surprised to discover thephenomenon of bacterial transformation. Look at the second and third panels of Figure 16.2. Based on these results,what result was he expecting in the fourth panel? Explain.
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Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
VISUAL SKILLS Griffith was trying to develop a vaccine
for S. pneumonia when he was surprised to discover the
phenomenon of bacterial transformation. Look at the second and third panels of Figure 16.2. Based on these results,
what result was he expecting in the fourth panel? Explain.
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- question: Can you summarize and explain for me what you want to tell in the article below? When I read it myself, I do not understand exactly what is meant by the article. It would be nice if you could highlight the important points. You can use them in a figure or diagram to explain. thank you and hava a nice day :) Article: Nanomaterial-Based Vaccine Development and Immunomodulation Following the publication of the genetic sequence of SARS-CoV-2 on January 11, 2020, intense research efforts have been devoted to developing a vaccine against COVID-19. With unprecedented speed, this extraordinary scientific mobilization led the first vaccine candidate to enter the Phase I human clinical trial on March 16, 2020, and other novel candidates are rapidly following. Up to May 22, 2020, there are 10 COVID-19 candidate vaccines in clinical evaluations and 114 in preclinical development. Concerning vaccine and immunization research, nanomaterials can assist in multiple ways to boost the…PART C (cont'd) Which of the following steps (on this page and the next page) would be necessary to determine the most effective antibiotic for a bacterial infection using the Disk Diffusion Assay? Select three necessary steps and three unnecessary or incorrect steps, and explain why it is or is not necessarv/correct. Collect a sample of bacteria from the infected patient. Collect samples of bacteria from the bathroom taps in the patient's room. Collect samples of bacteria from other patients who seem to have the same infection. Soak disks in many different types of antibiotic. Soak disks in the antibiotic you have the biggest supply ot. Label the disks with the type of antibiotic you soaked them in.PART C (cont'd) Which of the following steps (on this page and the next page) would be necessary to determine the most effective antibiotic for a bacterial infection using the Disk Diffusion Assay? Select three necessary steps and three unnecessary or incorrect steps, and explain why it is or is not necessarv/correct. Collect a sample of bacteria from the infected patient. Collect samples of bacteria from the bathroom taps in the patient's room. Collect samples of bacteria from other patients who seem to have the same infection. Soak disks in many different types of antibiotic. Soak disks in the antibiotic you have the biggest supply ot. Label the disks with the type of antibiotic you soaked them in. • Leave the petri dishes uncovered while the bacteria grow. , cover the petri dishes while the bacteria grow. Treat the bacterial culture with a disinfectant before spreading on the agar plate Wear personal protective equipment (gloves, mask, lab coat) while performing the procedure Wear…
- File Diffusion in the lungs and i View Edit Untitled + 1) What is dna replicate, define the process in detail. 1Graph SEQ Graph ARABIC I p24 po/ml 10 2) Quantitative skills: Using the graph below, determine the approximate concentration of p24 capsid protein (in kg/L) after 6 đays of transfection with the 168.1/R molecular clone. Include your calculations within the following space: 6 8 10 12 14 days after transfectionquestion: Can you summarize and explain for me what you want to tell in the article below? Can you explain the figure? When I read it myself, I do not understand exactly what is meant by the article. It would be nice if you could highlight the important points. You can use them in a figure or diagram to explain. thank you and hava a nice day :) Article: Nanotechnology Tools to Detect SARS-CoV-2 Standard procedures for detecting the virus from nasopharyngeal and/or oropharyngeal swabs have been reviewed recently and are primarily based on reverse transcription polymerase chain reaction (RT-PCR). Here, we would like to mention some preliminary ideas on nanotechnology-based assays to monitor the presence of SARS-CoV-2. A simplified test and variants thereof to detect viral proteins (e.g., HIV or influenza virus) without the need for expensive equipment is based on the color change of Au NPs bound to antibodies. Similar to the enzyme-linked immunosorbent assay (ELISA) antibodies coupled…
- Pipetting 1. Explain why a micropipettor is an important instrument in biochemistry labs. 2. Describe the basic components (and function) of a micropipettor. Bacterial Techniques 1. Define the following. (a) Serial Dilution (b) Streak Plating (c) Spread Plating Transformation 1. Define bacterial transformation and explain why it is an important method in biochemistry labs. 2. Describe (figure, narrative) a plasmid and describe the basic components of a plasmid. 3. What role does CaCl2 play in bacterial transformation? 4. What role does heat shock play in bacterial transformation? Plasmid Isolation 1. Describe the roles the following play in plasmid purification. (a) Lysis Buffer (b) Neutralization Buffer (c) Elution Bufferquestion: Can you summarize and explain for me what you want to tell in the article below? When I read it myself, I do not understand exactly what is meant by the article. It would be nice if you could highlight the important points. You can use them in a figure or diagram to explain. thank you and hava a nice day :) Article: Photodynamic Inactivation of SARS-CoV-2 In addition to drug- and vaccine-based antiviral strategies, photodynamic therapy (PDT) stands as a unique approach to inactivate SARS-CoV-2. Using a light-based method, PDT attacks target cells via the excitation of photosensitive agents, called photosensitizers (PSs), with radiation characterized by a wavelength corresponding to its absorption spectrum to generate reactive oxygen species (ROS) in the presence of oxygen, which ultimately results in cell death. Photodynamic therapy is primarily used for the clinical treatment of various oncological disorders. It was not until the 1970s that PDT was first used clinically…The scale bar in panels (a) and (d) in this image is 50 um (micrometei. Question: Which of the answers below is equivalent to 50 um. Figure description: An immunofluorescence image of uninfected cells (top panel a-c) and virus infected cells (bottom panel d-f). a,d) show Hoechst staining (blue) of the nuclei and a merged image of the respective panels: E staining for the virus (red); and c,f) show staining for calreticulin (green). The scale bar (white line) represents 50 um. Hoechst/Merge Virus Calreticulin a C d e O50m 50 x 10 m 50 x 10m 50 x 10 m + Virus Uninfected cells
- Available whis.picture. https://www.phys.ksu.edu/gene/photos/sd.html.g will help vou imagine the results for plateComplete the following tasks. You discovered that a species of bacteria can break down StyrofoamT (polystyrene) products due to an enzyme it produces, polystyrenase. You wish to study the gene that codes for this enzyme. Task 1: DNA Extraction To begin work on the bacterium, you begin by extracting its genomic DNA (GDNA). What is the purpose of the following procedures? Answer briefly but completely. Using sodium dodecyl sulfate, a detergent Answer: а. b. Adding RNase A and Proteinase K during extraction Answer: c. Adding ethanol before recovering the DNA extract С. Answer: Task 2: Polymerase Chain Reaction After purifying the gDNA extract, you want to isolate and amplify the polystyrenase gene. You perform PCR using the appropriate gene-targeted primers. What is the purpose of the following PCR components? Answer briefly but completely. DNA polymerase isolated from Thermus aquaticus Answer: а. b. Deoxynucleotide triphosphates (DNTPS) Answer: С. Forward and reverse primers Answer: Task…וןווד ← Q Lab Report 6 worksheets 314 F22 .DOCX File Edit View Insert Format Tools Help A 100% ¿ Summary Grades for Arysta Visser: 23 x M Uh-oh! There's a problem w X b The restriction EcoRI cleaves X + Untitled spreadsheet - Goog X https://docs.google.com/document/d/1mKY1HIgMPRh1kRDCmX7msBF2yf07-ogT/edit Outline Headings you add to the document will appear here. Normal text Times ... 12 + B I U 2 18. The restriction EcoRI cleaves double-stranded DNA at the sequence 5'-GAATTC-3', the restriction enzyme HindIII cleaves at the sequence 5'-AAGCTT-3', and the restriction enzyme BamHI cleaves at 5'GGATCC-3. An 805 bp circular plasmid is digested with each enzyme individually and then in combination, and the resulting fragment sizes are determined by means of electrophoresis. The results are as follows: 1 Restriction Enzyme(s) EcoRI BamHI HindIII EcoRI and BamHI EcoRI and HindIII BamHI and HindIII 3 Practice ====•=•€ EX Fragment lengths (base pairs) 430 bp, 375 bp 470 bp, 335 bp Lab Report 6…