Methods 1 GIow cels for seeral generations in medium that contains amino acids [2 Transferthe cells to a medium that contains amino acids labeled with a light isotope. labeled with a heavy isotope. Change medium Replication Isolate 3 Isolate histone ctamers before and after replication... Isolate octamers octamers 4..and subject them to dersity gradient centrifugation. Spin Spin 5 Newly synthesied octamers are less dense and thus will be Results higher in the tube. 6od octamers are dense and Single band; old octamers Broad band; octamers with mixture of old and new histones will move toward the bottom of the tube. with heavy amino acids (heavy and light amino acids)
Methods 1 GIow cels for seeral generations in medium that contains amino acids [2 Transferthe cells to a medium that contains amino acids labeled with a light isotope. labeled with a heavy isotope. Change medium Replication Isolate 3 Isolate histone ctamers before and after replication... Isolate octamers octamers 4..and subject them to dersity gradient centrifugation. Spin Spin 5 Newly synthesied octamers are less dense and thus will be Results higher in the tube. 6od octamers are dense and Single band; old octamers Broad band; octamers with mixture of old and new histones will move toward the bottom of the tube. with heavy amino acids (heavy and light amino acids)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
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What results would be expected in the experiment outlined in Figure if, during replication, all the original histone proteins remained on one strand of the DNA and new histones attached to the other strand?
Transcribed Image Text:Methods 1 GIow cels for seeral
generations in medium
that contains amino acids
[2 Transferthe cells to a medium
that contains amino acids
labeled with a light isotope.
labeled with a heavy isotope.
Change
medium
Replication
Isolate
3 Isolate histone ctamers
before and after replication...
Isolate
octamers
octamers
4..and subject them to dersity
gradient centrifugation.
Spin
Spin
5 Newly synthesied octamers
are less dense and thus will be
Results
higher in the tube.
6od octamers are dense and
Single band;
old octamers
Broad band;
octamers with
mixture of old
and new histones
will move toward the bottom
of the tube.
with heavy
amino acids
(heavy and light
amino acids)
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