) What is the advantage of cryo-sectioning for antibody staining methods?  5) When preparing coverslips with cultured cells for immunofluorescence why is it  necessary to coat coverslips with poly-L-lysine or gelatin?

Are the bands indicated in Step 6 (SDS-PAGE) likely visualized by western blot or by a general protein stain (such as Coomassie or silver)?

The DNA fragments separated on an agarose gel can be visualised after staining with what?

1) If direct stains work so well, why would we ever use indirect stains? Give two reasons why you might choose to use indirect stains instead of direct stains?  2) Assume that two days (dye A and dye B) were applied to bacterial smear. The cells were stained by dye A while the slide background was stained with dye B. Which stain had an anionic chromogen? Which stain had a cationic chromogen?

how does polyacrylamide gel electrophoresis with SDS separate proteins ?