In order to ensure reliable result, arrange the following procedure with A being the first and F the last. 9. SequencingIn order to ensure a reliable result, arrange the following procedure with A being the first and F the last.Place each onion on the glasses filled with Distilled water, with 50% Substance A and 50% distilled water, with 50% Substance B and 50% distilled water.The base of the onions must be submerged in the solution.Record your data and analyze.А.В.Place the set-ups in a well-lighted area for 2-5 days.If the onion is too small for the glass, poke four toothpicks, equally spaced, around the circumference of the mid portion of the onion for support.С.D.Е.Choose onion bulbs without shoots. Cut the roots of the onion bulb, making sure that there are no new or old roots present.F.Count the number of roots and shoots that has grown and measure their length.

Mentioned steps of the procedure are performed to analyze the number of shoots and roots of onion.…

Answered: 9. Sequencing In order to ensure a…

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter5: Biomedical Electronics: Digital

Section: Chapter Questions

Problem 2Q

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3) Define gel electrophoresis, including its theory and application. Describe the steps of running gel electrophoresis using the following image. More detailed reading: https://www.sciencedirect.com/topics/medicine-and-dentistry/agar-gel-electrophoresis POWER SUPPLY CATHODE ELECTROPHORETIC BUFFER ANODE WELL SAMPLE AGAROSE GEL POWER SUPPLY CATHODE ANOCE HIGH MOLECULAR WEIGHT SPECIES LOW MOLECULAR WEIGHT ANALYTES
What can be the errors and limitations of Gel Electrophoresis practical? Explain in very much detail and also provide some better suggestions.
The following are errors that people commonly make when they perform serial dilutions. Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you make this mistake. You intend to add 0.9 ml of diluent to each tube and 0.1 ml of culture. Instead, you add 0.5 ml of diluent to each tube and 0.1 ml of culture to the first tube. Then, you make a serial dilution of 0.1 ml into and from each tube as described. You prepare 0.9 ml of diluents in each tube. You add 0.1 ml of culture (from the overnight culture provided) to every tube. You add 0.9 ml of diluent to each tube. You add 0.1 ml of culture to the first tube and mix. You get distracted, and transfer 0.1 ml to the third tube instead of the second. You perform the rest of the series as described.
Gel used in electrophoresis is/are: a. Agarose gel b. Polyacrylamide plain gel c. Polyacrylamide SDS impregnated Polyacrylamide gel (Sodium dodecyl sulphate)
A nurse reconstituted a vial of 750 mg Cefuroxime Sodium Powder for Injection with 6mL of sterile water for injection. The reconstituted solution was dark amber-colored solution. The package insert states that solution colors range from clear to yellow depending on concentration, diluent, and storage conditions. The nurse was then hesitant to give the patient the solution due to its unusual dark color. Five portions were taken from a batch of cefuroxime sodium. Prior to testing in the instrument, each part was subjected to one of the following conditions: Conditions Specifications Temperature Portion 1: 8°C ± 2°C Portion 2: 30°C ± 2°C Portion 3: 40°C ± 2°C Light Portion 4: Kept in the dark Portion 5: Exposed to direct sunlight 1. Which form of the product – the powder for injection, the reconstituted product, or both – will you subject to the following conditions? Why do you think these forms can best address the problem?
6) 1 mL of supernatant is required for a procedure. The final colored solution proves to be too high to read accurately on the spectrophotometer. 100 μL of supernatant and 900 μL of distilled water are substituted for the original supernatant and the procedure, run as before. The reading from the standard curve is 46 mg/dL. What is the actual amount of substance in the patient serum?
What is the experimental procedure illustrated in the figure above? What label (1-3 words) would you write next to each of the 5 arrows in the procedure? ) Briefly write a concise but complete description of the figure closely following the labels you have used in your answer to the second part of the question.
Discuss the principles and applications of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry in molecular diagnostics.
Which precise standards are you going to apply in order to identify your unknown? Make sure your RFP can be clearly distinguished from the other two using the criteria. lab intro attcehd if needed:
1mL Stock #1 1mL 2000060 9mL #2 9mL wwwww #3 4. 1mL 0000000 A 9mL wooooo #4 0.1m/L O 1mL 5000000 B 9mL #5 1mL 1mL 9mL wwwwww #6 0.1mL 1mk O. D Using the picture serial dilution scheme and the following information (plate A has 276 colonies, plate B has 298, plate C has 2, and plate D has 30), calculate the average number of colony forming units per mL in the stock tube. Make sure to only use countable plates. Round your answer to the nearest one. Write only the number with any needed commas or decimals. Do not include units.
Identify the following on the gel electrophoresis 2-leg 2-log ladder 3 Kb 1022 bp 1022 bp 765 bp 880 bp 765 bp 1 Kb S00 hp 100 bp sample given
How can you make a final dilution of 10-10 using 5 dilution tubes? Outline your steps.

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