Using CRISPR/Cas9 to target a gene in the mouse will primarily result in: a. Any of the choices b. Synonymous substitutions C. Homozygous gene knockouts

siRNA and CRISPR/Cas9 each interfere with gene expression by a sequence-directed mechanism. A. Identify the target macromolecule (DNA, RNA or Protein) of a specific siRNA/RISC andCRISPR/Cas9 complexsiRNA:CRISPR/Cas9: B. If a scientist would like to permanently turn off expression of a particular human gene inthe liver for the lifetime of an individual, which of these techniques would bepreferable? Briefly explain why

Answer the following questions about CRISPR below: A.What is a PAM sequence? B.How does the Cas-9/RNA sequence target a piece of DNA for destruction? C.How is the DNA repaired after being cut by CRISPR/Cas-9?

Site-directed mutagenesis can be used to: a.   treat diseases by gene therapy.   b. make single residue mutation of a protein. c. make human insulin for treatment of diabetes. d. determine protein structures. e. determine DNA sequencing

Choose the item in column 2 that best matches each item in column 1. A. Provides information for production of protein B. Binds to promoter C. Promoter has a box A and box B consensus sequences D. Autocatalytic RNA molecules E. Associated with transcription termination F. Polymerization of ribonucleotides to form RNA molecules G. 7-methyl guanosine H. MRNA prior to processing select - 1. Core RNA polymerase select 2. rho (p) factor select 3. hnRNA select - 4. RNA select -5. RNA polymerase holoenzyme select 6. Ribozymes select - 7. 5' MRNA cap select 8. MRNA

Which construct would you use to make a mouse model ?

Differential RNA splicing may result in: a. A shift in the ratio of mRNA produced from two neighboring genes b. Inversion of exons in the mature mRNA c. Production of the same protein from two different genes d. Production of two different proteins with different properties from a single gene

Which of the following is NOT a DIFFERENCE between prokaryotic and eukaryotic gene regulation? A. prokaryotic mRNA is NOT capped by a 5’mG after transcription, but eukaryotic mRNA is so capped B.  eukaryotic mRNAs are monocistronic (encode single proteins), whereas prokaryotic mRNAs are polycistronic (encode multiple proteins) C.  prokaryotic mRNA is not modified by polyadenylation after transcription, but eukaryotic mRNA is so modified. D.  translation of eukaryotic mRNA into protein is not coupled to transcription of the mRNA from DNA, but in prokaryotes it is so coupled E.  prokaryotic translation and eukaryotic translation use different genetic codes to translate mRNA codons into amino acid sequences of proteins