The goal of most gene therapies is to insert a healthy copy of a gene into the genome.  Besides CRISPR, techniques for integrating DNA into the genome do not target that DNA to a particular genomic location and thus different cells end up with the DNA sequence in different positions. B.     The same gene integrated into different locations in the genome will be expressed differently.  Why?

Q: What is a genomic library? O collection of sequences made of DNA fragments O collection of vectors…

A: The molecule that contains the genetic information necessary for an organism's growth and…

Q: In automated sequencing, you are given a printout of the sense strand of your DNA.  the first thing…

A: Reading frame is a method to read the DNA sequence to decode the amino acids sequence.

Q: You are studying a human gene, and try to express the protein in E. coli bacterial cells. To do…

A: Cloning is the process of creating a genetically identical copy of a biological entity. Cloning can…

Q: What color is the alpha gene spot on the microarray? Answer: Yellow please explain why is the…

A: The microarray can be described as a lab-on-a-chip. It can be used to detect the expression of…

Q: One concern about using genetically-modified organisms is that many of the methods used to create…

A: CRISPR/Cas9 technology can be used to introduce targeted mutations or deletions in the genome by…

Q: Besides the great potential of gene editing, what are the biggest risks?  How do scientist test to…

A: Gene editing is a rapidly advancing field of genetic engineering that allows for precise…

Q: a. Using nucleotide letters, show the kind of cut that could be made on a DNA molecule to…

A: The plasmid is a small, circular deoxyribonucleic acid apart from the main bacterial DNA. Plasmids…

Q: Cancer cells were characterized for their high transcriptional and translational levels. Direct…

A: Transcription factor – a material, such as a protein, that contributes to the purpose of a specific…

Q: Northern blotting, RT-PCR, and microarrays can be used to analyze gene expression. A lab studies…

A: Gene expression is similar to protein expression and it is mediated by transcription and translation…

Q: Match the activity below with the correct enzyme. (You won't use all the enzymes listed.) RNA acts…

A: DNA helicase is used during the DNA replication process where it binds to the double-stranded DNA…

Q: The statement below may be true or false. If it is true then type "true" in the box below. If it is…

A: DNA that is silenced can not bound by transcrpition fators

Q: In a clinical context, doctors are evaluating a therapy for a new patient (say patient B) that they…

A: In the setting of assessing treatment for a modern patient (patient B) who may develop comparable…

Q: Explain How Single-nucleotide mutations can be introduced into the genome using an engineered…

A: According to the question, we have to explain how single-nucleotide mutations can be introduced into…

Q: Researchers create a recombinant DNA molecule in which the coding sequence for GFP is inserted…

A: Recombinant DNA is the DNA that is formed by insertion of gene of interest into the original DNA.…

Q: here is a hypothetical gene related to the nervous system of Drosophila. Describe all the methods,…

A: In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA, RNA or…

Q: Positional cloning refers to: a. using a selection procedure to clone a cDNA b. cloning a portion of…

A: Positional cloning is a gene cloning method where cloning is done simply based on the knowledge of…

Q: Ⓒ Macmillan Learning Identify sequence of target gene Verify absence of target gene by PCR Answer…

A: Introduction :- Certain viral infections and genetic alterations can be quickly and accurately…

Q: What is homologous recombination and where/when does it typically take place in an organism.…

A: Homologous recombination is a genetic exchange process that occurs between two similar or identical…

Q: Site directed mutagenesis is used to: 1.determine the critical base per sequences in a Genome 2.…

A: DNA consists of double strands of sequentially arranged nucleotides. The nucleotide sequence present…

Q: You are trying to study the effects of Drug A on the expression of Gene X in a tumor sample (lane…

A: Reverse transcription enzyme chain reaction (RT-PCR) may be a variation of normal PCR that involves…

Q: a. The reading frame DNA sequence is  b. The mRNA sequence is  c. The polypeptide sequence is…

A: A "nucleic acid" is a linear polymer of nucleotides that is a component of the cell's information…

Q: a. Why doesn't Dpnl digest the mutagenized DNA?  b.What is the approximate size, in base pairs, of…

A: a. Why doesn't Dpnl digest the mutagenized DNA?  b.What is the approximate size, in base pairs, of…

Q: 1. Discuss the genetic potential of the cloning of single genes, cells, organs, and entire…

A: Gene cloning Gene cloning is a molecular technique by which a gene of interest is isolated and…

Q: The experiment below is from a seminal set of experiments in the 1960s that illustrated the role of…

A: DNA is a double-stranded molecule that carries genetic information. It consists of nucleotides with…

Q: Primers can sometimes bind and target the wrong gene, especially if the primers are allowed to bind…

A: It is difficult for one to to find out gene of interest out of a complete genome. However, a given…

Q: The CRISPR system may be used to manipulate the genome in human cells, because Cas9 can

A: CRISPER system can use Cas protein to manipulate the genome present in human cell.

Q: 6. Suppose a particular gene is required for early development and also later for development of a…

A: ANSWER;- The Gal4 (galactose) gene articulation is directed by Gal80. The Gal4 gene protein…

Q: If you are a genetic engineer and you cloned your gene of interest in a plasmid and you want to know…

A: Answer. Blotting describes the immobilization of sample nucleic acids/proteins onto a solid support.…

Q: Introducing a specific change in the mouse genome using CRISPR/Cas9 requires: a. Alterations to both…

A: CRISPR based genome editing is widely applied in the field of biotechnology, medicine, genomic…

Q: Bacteria exposed to viruses incorporate sections of the virus’s DNA into the CRISPR array sequences…

A: Answer  when a bacteria is infected with bacteriophage is incorporates a small segment into its…

Q: Some people have argued that editing the genomes of human embryos is ethically defensible as long as…

A: Genome editing tools are those tools that help in making changes into the genome of an individual.…

Q: A partial DNA sequence, a partial MRNA transcript, and part of the CFTR polypeptide in a cystic…

A: Cystic fibrosis is a disesase that damage the cell producing mucus, sweat and other juices.  in…

Q: The goal of most gene therapies is to insert a healthy copy of a gene into the genome.  Besides…

A: The expression of a transgene depends on its site of integration, state of differentiation of target…

Q: Genetically modified Atlantic salmon have recently been approved for human consumption. These…

A: CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) refers to a specific DNA sequence…

Q: has been assembled by researchers and transplanted into a donor bacterial strain to study never…

A: A- recombinant plasmid  B- developmental genetics

Q: The Ames Test... a. is always used to detect cancer-causing virus b. has the potential to completely…

A: Ames test: A test to determine the mutagenic activity of chemicals by observing whether they cause…

Q: You are studying celiacs disease and have identified a potential gene for the disease using linkage…

A: Genetics is the study of the heredity, expression of genes and control of phenotypes. There are…

Q: Q. How can you design your RT-PCR experiment to control for gDNA contamination? A. Use forward and…

A: Polymerase Chain Reaction (PCR) is a technique that gives us multiple copies of desired DNA…

Q: a. Describe two ways you could potentially make atransgene that would inhibit the function of a…

A: A transgene is a gene, which is transferred from one organism to another organism naturally or…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.