Describe one way in which DNA double stranded break repair that occurs because of X-ray damage differs from that caused by Crispr/Cas9 and one way in which they are similar.

Q: Describe the experimental evidence that DNA is thehereditary material of bacteriophages.

A: Viruses are obligate parasites. They are non cellular entities which have their RNA (ribonucleic…

Q: Does one have a practical idea of the role of CRISPR/Cas9 in genome editing via cutting of DNA?

A: A molecular process in which some discontinuous change to nucleotide sequences in their genomic DNA…

Q: How does one performs a Southern blot and how one performs a western blot? Then, consider what a…

A: The transfer of biomolecules like protein, DNA, or RNA from gel to the carrier blotting membranes is…

Q: CRISPR-Cas9

A: Both statements are true.

Q: Which of the following functions can be fulfill Form sticky ends to insert genes Create mutations in…

A: Proteins are the large biomolecules and macromolecules that comprise one or more amino acids or the…

Q: Bacteriophage lambda Describe the method used to retrieve your gene of interest from the plaques.

A: The genomic library is critical in genomic investigations, whether to determine the link between…

Q: Describe the terms: Transformation Operon Recombination

A: Hello you have asked question with multiple subparts. We will be able to answer only first three.…

Q: Which of the following types of mutations would be produced by repair of a CRISPR-targeted double…

A: Non Homologous End joining is described as the pathway required for the repairing of those double…

Q: Explain the difference between the potential effects on an individual organism of errors made during…

A: During DNA replication, proofreading occurs when DNA polymerases fixes the majority of mismatched…

Q: Which of the following statements about the CRISPR-Cas9 technique is correct? 1. Catalytically…

A: CRISPR-Cas9 (Clustered regularly interspaced short palindromic repeats-CRISPR associated protein) is…

Q: In CRISPR genome editing technology, when the Cas9 protein cleaves the target DNA sequence, what…

A: The microorganism depends on multiple defense strategies that support them to resist viral attack.…

Q: Explain How Single-nucleotide mutations can be introduced into the genome using an engineered…

A: According to the question, we have to explain how single-nucleotide mutations can be introduced into…

Q: The Cas9 enzyme requires a short sequence called a PAM (Protospacer Adjacent Motif) for cleaving the…

A: A protospacer adjacent motif (PAM) is a 2–6 base pair DNA sequence tareted by Cas9 nuclease.

Q: Contrast the functions of Spo11 and Cas9, two enzymes that catalyze the formation of double-strand…

A: A double-strand DNA break (DSB) occurs when both strands of the DNA duplex are damaged as the result…

Q: How does homologous recombination with transfected disruption constructs can inactivate specific…

A: The plasmid does not contain the yeast origin of replication. The gene target constructed to…

Q: Reverse transcriptase has ribonuclease activity as well as polymerase activity. What is the role of…

A: The biological catalysts that speed up the biochemical reactions are called “enzymes”. They act on…

Q: Which of the following is a correct statement about CRISPR-Cas-9 gene editing? Group of answer…

A: CRISPR-Cas9 is a widely used genome editing technology as it enables scientists to alter genes in…

Q: Describe one specific application of CRISPR-Cas9 that is either currently in use or could…

A: Genome editing is a technology that can change the genome of an organism. This technique allows the…

Q: Give at least 3 ways an organism can counteract DNA damage and mutations by activating several types…

A: DNA is the genetic material present in the nucleus of eukaryotes.

Q: Based on the following wild type DNA sequence, indicate if each of the mutations should be…

A: A mutation is a change in a DNA sequence. Mutations can result from DNA copying mistakes made during…

Q: List two possible reasons why a mistake during the process of replication is more of a catastrophe…

A: When a DNA (deoxyribonucleic acid) molecule (double-stranded) gets copied into two new similar…

Q: . In prokaryotes and eukaryotes, describe what else is happening to the RNA while RNA polymerase is…

A: RNA synthesis, which is carried out by RNA polymerase, maybe a basic movement in both prokaryotes…

Q: what are the mechanism of CRISPR-Cas-9  to combine DNA? explain.

A: Through the introduction of genes from closely related species, it is possible to employ genome…

Q: Explain how gene conversion can occur via mismatch repair and DNA gap repair synthesis.

A: Step 1 Gene conversion is the process of replacing a homologous sequence with a deoxyribonucleic…

Q: Which of the following protospacer adjacent motif sequences would be recognized by Cas9?     GCG…

A: Cas9 stands for CRISPR (clustered regularly interspaced short palindromic repeats) associated…

Q: "Knocking out" a gene with CRISPR-Cas9 leads to which of the following? O disruption of a gene O…

A: CRISPR-Cas9 is a unique technology that helps geneticists and researchers to edit a particular part…

Q: Explain why retroviral integration systems are better than the bacterial recombination system.

A: Bacterial recombination is formed when the new DNA is the transfer from the donor cell to the…

Q: Explain the function of CRISPR in bacterial cells.

A: Introduction CRISPR is a group of DNA sequences present in the genomes of bacteria and archaea,…

Q: Which of the following statements is not true about the guide RNA (see this interactive…

A: The objective of the question is to identify the incorrect statement about guide RNA in the context…

Q: How can the efficiency of homologous recombination be improved when using CRISPR-Cas9, compared to…

A: CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a technique derived from…

Q: What are bypass polymerases? How do they differ fromthe replicative polymerases? How do their…

A: Polymerases are enzymatic proteins that has a specific role in maintaining the overall molecular…

Q: Which component of the CRISPR-Cas system directly recognizes thebacteriophage DNA?

A: CRISPR(Clustered regularly interspaced short palindromic repeats) is a region of DNA present in…

Q: 10) Scientists have engineered a simplified CRISPR-cas system for use in mammalian cells. What were…

A: The clustered regularly interspaced short palindromic repeats (CRISPR) is a technology that is used…

Q: Generate a concept map that includes all the specifics below: • Classification based on: 1. their…

A: The full form of DNA is deoxyribose nucleic acid. It carries genetic information from one generation…

Q: Knock out mice that are mutant for a gene product X die. Variants of the X gene that were…

A: The physical crossing over during meiosis of parental heterozygous homologous chromosomes or genes…

Q: Describe several aspects about RNA polymerases that are unique compared to DNA polymerases.

A: Many processes on the cellular level need specific enzymes to carry out or catalyze the process. RNA…

Q: describe Site-Specific Recombination

A: Site-specific recombination moves specialized nucleotide sequences between nonhomologous sites…

Q: CRISPR-Cas9 can be used by scientists to edit genes. This system was not created in a lab, however,…

A: CRISPR-Cas 9 (Clustered Regularly Interspaced Short Palindromic Repeats) is a natural mechanism of a…

Q: Genetically modified Atlantic salmon have recently been approved for human consumption. These…

A: CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) refers to a specific DNA sequence…

Q: CRISPR/Cas9 mediated genome editing has provided an unparalleled means of manipulating the genome.…

A: CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats/CRISPR-associated genes) is a…

Tissue regeneration & repair

Tissue regeneration involves the restoration of tissue components by regrowth of damaged tissues. In contrast, tissue repair involves patching of injured tissue rather than restoring it. It is the proliferative capacity of the cells that decides the amount of regeneration against the repair that would occur in the tissue. Both regeneration and repair depending on the duration of the injury and response to the inflammation.