Use the numbers below to indicate the correct order of events (from left to right) during the process of translation initiation in bacteria.   large ribosomal subunit binds to the complex. IF3 is released. The small ribosomal subunit associated with IF1, IF2, and IF3 binds to the Shine-Dalgarno sequence on the mRNA. tRNA charged with f-methionine binds to the AUG codon at the P site. IF1 and IF2 are released, leaving the A site available for the second charged-tRNA to bind the codon at the A site.     3 1 5 4 2 2 1 4 5 3 1 3 5 2 4 3 4 2 1 5 4 2 1 3 5

An RNA polymer is made by using the enzyme polynucleotide phosphorylase with equal quantities of CTP and GTP.  When this RNA is used in an in vitro translation system, all of the following amino acids could be incorporated into a newly made polypeptide, except:     glycine (Gly) histidine (His) proline (pro) alanine (Ala) arginine (Arg)

Indicate the amino acid sequence of the protein encoded by the following mRNA molecule.  Use the genetic code table and assume that the very first “AUG” the ribosome encounters will serve as the start codon and specify methionine. 5’-AAUUCAUGCCCAAAUUUGGGGCACGAAGCUUCUUAGGCUAGUCCUAAAAAA-3’

Order the following choices to indicate the sequence of steps in the process of elongation of a growing peptide chain. Peptidyltransferase reaction catalyzed by large rRNA subunit. Aminoacyl-tRNA binds the ribosomal A site via EF1x-GTP hydrolysis. EF2-GTP hydrolysis promotes translocation of the ribosome. Release of EF1«-GDP. >

What is the correct sequence in translation? a   tRNA attaches to start codon, ribosome attaches to mRNA in P site, new tRNA enters on A site, peptide chain moves to old tRNA in P site, old tRNA moves to E and new tRNA to P. b   tRNA attaches to start codon, ribosome attaches to mRNA in P site, new tRNA enters on A site, peptide chain moves to new tRNA, old tRNA moves to E and new tRNA to P. c   tRNA attaches to start codon, ribosome attaches to mRNA in A site, new tRNA enters on P site, peptide chain moves to new tRNA, old tRNA moves to E and new tRNA to A. d   tRNA attaches continuously to mRNA without the need of a ribosome. Proteins are: a   the direct translation of a single gene b   in most cases, the results of post-translation control c   first transcribed from DNA by an operon d   transcription factors Which of the following statements is false? a   In the lytic cycle, new phage are produced and released into the environment. b…