TUBE 1 TUBE 2 TUBE 3 TUBE 4 TUBE 5 Volume pipetted Total volume Sample volume: Total volume (ratio) Individual dilution factor Total dilution factor
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- If 1.0 mL of a 1:1000 dilution produces 200 colonies on a culture plate, how many bacteria were present per mL in the original (undiluted) sample? 200,000 20,000 2,000 200d/e/1FAIpQLSfTle9UfP15_VUqFI-ACEQd1XBykXv5Lr4dEMQbLJ1d6fCupw/viewform Students subjected three samples of five different molecules to gel electrophoresis as shown in Figure 1 A B C DE +2 3 Wells 4 8. Which of the following statements best explains the pattern seen on the * gel with regard to the size and charge of molecules A and B? 1 point molecules A and B are positively charged, and molecule A is smaller than molecule B. molecules A and B are positively charged, and molecule A is larger than molecule B. molecules A and B are negatively charged, and molecule A is smaller than molecule B. molecules A and B are negatively charged, and molecule A is larger than molecule B. Sign outIdentify the following on the gel electrophoresis 2-leg 2-log ladder 3 Kb 1022 bp 1022 bp 765 bp 880 bp 765 bp 1 Kb S00 hp 100 bp sample given
- 1mL Stock #1 1mL 2000060 9mL #2 9mL wwwww #3 4. 1mL 0000000 A 9mL wooooo #4 0.1m/L O 1mL 5000000 B 9mL #5 1mL 1mL 9mL wwwwww #6 0.1mL 1mk O. D Using the picture serial dilution scheme and the following information (plate A has 276 colonies, plate B has 298, plate C has 2, and plate D has 30), calculate the average number of colony forming units per mL in the stock tube. Make sure to only use countable plates. Round your answer to the nearest one. Write only the number with any needed commas or decimals. Do not include units.A class of 15 students (8 males and 7 females) will need to culture an unknown bacteria for a specific activity where 5 nutrient agar pates per female student and 3 agar slants per males student are needed to prepare. agarplate: 25mL Agar slant: 10mL Broth tube: 8mL Nutrient Agar: Yeast extract: 2g/L peptone: 5g/L Sodium chloride: 5g/L Agar powder: 15g/LA 10-5 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 278 colony forming units grow on the plate. How many bacteria are in a single mL of the original culture? Express your answer to two decimal places using scientific notation. In scientific notation 540 would be written as 5.40*10^2. Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transferred, a dilution is being performed. Any time more than 1 mL is transferred, a concentration is being performed. Include the trailing zero so there are two decimal places Canvas expects a single digit before the decimal point. 5.40*10^2 is how Canvas expects 540 to be formatted in scientific notation 54.00*10^1 would be marked wrong. 0.54*10^3 would be marked wrong. A 10-5 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the…
- 1 ml from main culture 5 ml LB 1 ml 1ml 1ml 5 ml LB I ? 5ml LB 1 ml 5 ml LB 5 ml LB You took 250 µl of the last dilution and planted it on solid medium. 50 colonies were counted A total of 10 ml of microbial culture (liquid LB+ e.coli) was brought to your laboratory in a container. This sample contains the bacteria Escherichia coli. The person who brought in wants to know how many colonies (cfu) there are in the culture. You have started the experiment by taking 1 ml from the culture and then added into the first tube which contain 5 ml of LB. After that, you diluted the sample with a dilution rate of 1/6 with 4 times (take 1 ml in 5 ml of LB medium (so total 6 ml)). You took 250 μl of the last dilution and planted it on solid medium and when counted after incubation, 50 colonies were counted. How many cfu/ ml bacteria are there in the sample (main stock) in total? (please show all your calculations and steps). Attach FileCopy and paste the link below and watch the video on Youtube https://www.youtube.com/watch?v=8RBs0Ghg_48 Answer the following Questions: 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up 3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate? 6. What is the purpose of glycerol in the sample buffer? 7. What is the use of a DNA ladder? 8. What will happen when you increase the voltage of the set-up? 9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?You want to determine the amount of cells in a culture. You dilute the suspension to 10^-4 and plate 100ul onto an agar plate. After overnight incubation there are 30 colonies on your plate. How many cells are in your original suspension (assume your culture is 1 Liter)?
- What are the mechanisms of samples separation work in Thin layer Chromatography? Please shortly write at your own words. Answer should be to the point (5-6 lines maximum).You prepared a 7x 10^5x dilution from your bacterial culture, plated 0.2 ml of it on a Petridish and counted 67 cfu. What was the cell density of your bacterial culture (in cfu/ml? How many cells did you have in total if the volume of your culture was 50ml? Round to a whole number, do not write in scientific notation. The cell density of my bacterial culture was cfu/ml. The total number of cells wasWhat would the final concentration of a bacteria culture be if 2.7 x 106 cells/ml were diluted 8.2 x 10-2? What would the initial concentration of a bacteria culture be if the final concentration was 3.7 x 102 cells/ml and the total dilution was 4.6 x 10-4?