Directions: Suspend 28.0 grams in the 1000 mL distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. If desired the medium can be enriched with 5-10% blood or other biological fluids. Mix well and pour into sterile Petri dishes.

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