UESTION ANSWER 0.IM PIPES (pKa = 6,8) %3D mich of the following aqueous solutions has the highest pH? 0.1M HEPES (pKa = 7.6)
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- At what pH does a lysine solution exhibit the highest buffering capacity? (Lysine pKas: pk1 = 2.2, pK2 = 8.95, pK3 = 10.5) %3D O рH 12.1 О рH 9.72 O pH 5.67 pH 8.95pH of solution 14.00 12.00 10.00 8.00 6.00 First 4.00 equivalence point 2.00 0 First midpoint Second equivalence point Third midpoint pH = pKa=12.32 Third equivalence point HPO4(aq) + OH(aq) PO4(aq) + H2O(1) Second midpoint pH = pKa = 7.21 H2PO4 (aq) + OH(aq) HPO42 (aq) + H2O(1) Using the Henderson- Hasselback equation, show how to create 2L of a 0.1 M KPhos pH 7.5 buffer using K2HPO4 and KH2PO4. The chart to the left should help you understand what pKa to start with. Show your work. pH = pKa = 2.16 H3PO4(aq) + OH(aq) H2PO4(aq) + H2O(l) 25.0 50.0 75.0 100.0 Volume of NaOH added (mL)amino glycine pka is 2.4, 9.8 Calculate the most effective buffering range. if there is two pka, since there are 2 buffer region, is the buffering range 1.4~3.4 and 8.8~10.8? i would like to know the correct answer and detailed solution/reason.
- 7. · In the space provided, sketch a titration curve (pH vs H+ ions dissociated) of a phosphate buffer shown below. The pKa of H2PO4 is 7.0. H,PO, НРО, + 9. 8. 7 pH 3 1.0 0.5 H+ dissociated --> b. What is the effective buffering range of this acid? At what pH will you have 25% of the buffer in the form of H2PO4? с.How much of the enzyme proteinase k (solute) is required to make 250ml of a solution with a concentration of .01mg/ml? (Weight/volume ratio)In a 0.1000 M acetic acid solution at 25 degrees celsius , the acid ionizes to the extent of about 1.34 %. Since each molecule of acetic acid which ionizes produces 1 H+ ion and 1 C2H3O2- ion, the concentration in the solution are: HC2H3O2 < -----------> H+ + C2H3O2-
- The pH probe/meter uses following equations: Ecell = L + 0.0592 log a1 = L - 0.0592 pH Where L = L1 + EAg/AgCI + Easy= constants L1 = - 0.0592 log a2 a1 = activity of analyte solution a2 = activity of internal solution How will measured pH value be affected vs “real” pH if HCl in pH electrode, became 0.15M instead of 0.1M ? a.impact can not be determined b.measured pH is higher than "real" pH. c.measured pH is lower than "real" pH. d.measured pH is same as "real" pH.Calculate the concentration of Y4-ion in 0.0100 M EDTA solution at pH 6. α4 = 2.2 x 10-5 for pH 6.The pH probe/meter uses following equations: Ecell = L + 0.0592 log a1 = L - 0.0592 pH Where L = L1 + EAg/AgCI + Easy= constants L1 = - 0.0592 log a2 a1 = activity of analyte solution a2 = activity of internal solution How will measured pH value be affected vs “real” pH if the temperature of the sample is 30C when pH was measured ? a.measured pH is lower than real pH b.impact can not be determined c.measured pH is higher than real pH d.measured pH is same as real pH
- You just made a 1.5M permanganate solution. What concentration is your potassium permanganate solution in percent? "K: 39.10 g/mole" "Mn: 54.94 g/mole" "O: 16 g/mole" "MW of KMnO4 is 158.04 g/mole" O a. 0.24% Ob. 20.1% O c. 15.8% O d. 23.7% O e. 2%pH expresses the molar concentration of hydronium ions in an aqueous solution on a logarithmic scale. (Note that it is common to use the hydrogen ion, H*, to represent the hydronium ion, H3O+.) pH = -log[H3O+] [H3O+] = 10-PH As pH decreases, acidity increases. As pH increases, acidity decreases. • Acidic solution: pH 7 Part A - Calculating pH Submit [H₂0+] 1 Acidic pH 01 10¹ 102 103 104 105 106 107 108 109 10-10 10-11 10-12 10-13 10-14 stomach acid 3 wine lemon tomatoes juice vinegar, black soft drinks, coffee beer 6 Neutral urine milk 7 human blood pure water sea- water baking soda 10 11 12 13 14 milk of magnesia house- hold ammonia oven cleaner Basic household bleach Adapted from Biological Science by Freeman ©2008 Pearson Education, Inc. What is the pH of an aqueous solution with the hydronium ion concentration [H3O+] = 2 x 10-14 M? Make sure that your answer has the correct number of significant figures. For help determining the correct number of significant figures, see Hint 3. ►…Part 2 You decide to try anion exchange chromatography (DEAE column). To do this, you first equilibrate your column with column buffer (0.1M NaCI, pH 7.5). You then apply your desalted AS-P (Ammonium sulfate pellet) sample to the column, and collect the flow through (FT). Next, you elute the column with increasing concentrations of NaCl, and collect fractions (#1-5). Finally, you perform a quantitative assay of each fraction for activity. Below are three possible (independent) outcomes of this experiment. Explain what each outcome tells you about the conditions of the experiment under the conditions you ran each column. Make sure to detail the reasons behind each outcome (You may assume that the AS-P fraction had activity before you loaded it). Age A B. FI 12