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To achieve a hundred fold dilution you would add _____ microliters of sample to the appropriate amount of broth for a final volume of 1 mL.
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- Using 10 μL of a patient's serum, how much diluent would be needed to create a 1:10 dilution? ______ μLA plate has 72 colonies with a total dilution factor of 10-7, 0.1 ml was pipetted onto the plate, what was the original CFU/ml concentration of that sample?Using 25 μL of serum, how much diluent would be needed to create a 1:2 dilution? ______ μL
- Label three sets of eight microfuge tubes (1-8) and add the volumes of BSA stock solution (2 mg/ml) and water to each labeled tube as indicated in Table 3. Be sure to calculate, and enter, the values for column 4 of this table. Table 3 STD Curve Volume BSA Volume Water (µl) Final BSA Concentration tube (mg/ml) Stock (ul) number 1 100 2 95 3 10 90 4 20 80 5 40 60 6 60 40 7 80 20 100 00A sample of pond water is tested using the most probable number method. There are 3 positive tubes for 10 mL, 1 positive thbe for 1 mL, and 1 positive tube for 0.1 mL. the most probable number per 100 mL is ________ ? fill in the blank.create a serial dilution from a 1.5 x 10^8 CFU/ml stock solution that will give you 1500CFU/ml when you plate it.
- Strong Iodine Solution USP Formula: Iodine pellets ____Potassium iodide ____Purified water ____To make 60ml Procedure:Draw a serial dilution that would give a dilution of 1/153 000 000. Each tube in your dilution series must have a volume of 50 ml or less.To the right is an image of a dilution that was performed. The volume above the top arrow indicates the volume that should be transferred to the next tube (i.e. Tube 1). The volume listed at the bottom-right of tube 1 indicates the volume of diluent that should be added to that tube. The stock concentration is 50μg/ml and you want to make a solution in tube 1 with a concentration of 0.4μg/ml and a total volume of 3ml. Stock ?ml Tube 1 ?ml a. How many milliliters of stock solution needs to be added to Tube 1? Round your answer to three decimal places (e.g. 0.111). b. How many milliliters of diluent needs to be added to Tube 1? Round your answer to three decimal places.
- Choose the one answer that fits best. Which of the following statements regarding the proper procedure for using Micropipettes is NOT correct? O a. You cannot use a 2-20 µl micropipette to pipet 200 µl O b. To expel all the liquid from the tip, you have to press the eject button O c. To draw up solution, press and hold the plunger at the first stop before entering the solution O d. Micropipettes always require the use of a disposable plastic tip O e. While pipetting, micropipettes should always be held as straight as possibleHow many CFU per ml are there in the original sample using the following dilution and platingseries? Make sure to label all dilutions that were done.Immediately after stirring 1 hour after stiring Original misture Suction filiration Figure 1 Figure 2 Figure 3 Various tests are performed on the mixtures as shown in the figures above. Identify any correct explanations of these tests below. [Check all statements that are true.] In figure 1, a laser is passed through two tubes of clear liquid. The liquid in the tube on the left is a solution, while the liquid in the right tube is not a solution. O In figure 2, a recently stirred mixture is allowed to stand for one hour. The original mixture is a solution while the mixture after 1 hour is not a solution. In figure 3. a mixture is filtered using suction filtration. A solid remains on the filter. The original mixture was a solution.