What are the mechanisms of samples separation work in Thin layer Chromatography?
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What are the mechanisms of samples separation work in Thin layer Chromatography? Please shortly write at your own words. Answer should be to the point (5-6 lines maximum).
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- What is Dry Flash Column Chromatography? Please explain at your own words( around 500 words).0.9% salt (NaCl) concentration is isotonic to red blood cells. What would happen to a patient's red blood cells if she accidently received an intravenous fluid in the hospital that was made with 0.09% salt solution Edit View Insert Format Tools Table 12pt v Paragraph v B I U AV e T?vYou want to set up a 1:10 dilution series, so that you can plate a 10-1, 10-2, and a 10-3dilution; however, you only have access to two9 ml dilution blanks. Explain how you could accomplish this task with only two blanks.
- The usual dose of digoxin for rapid digitalization is a total of 1.0mg, divide into two or more portion at intervals of 6 to 8 hours. How many milliliters of digoxin elixir containing 50mcg/mL would provide this dose?Copy and paste the link below and watch the video on Youtube https://www.youtube.com/watch?v=8RBs0Ghg_48 Answer the following Questions: 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up 3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate? 6. What is the purpose of glycerol in the sample buffer? 7. What is the use of a DNA ladder? 8. What will happen when you increase the voltage of the set-up? 9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?Image 1 shows raw data of gel electrophoresis. Label/annotate image 1 (lanes, ladder sizes, etc). Explain what your seeing in the gel. Use the following information and picture 2 to assist in labelling. The purpose of this gel electrophorsis is to ensure that your GOI, FAP257, is in each BAC. Protocol that was done for Gel electrophoresis: -Place tray with gel into gel box -Fill gel box with IX TAE until the gel is completely submerged -Remove the comb for wells -Load 10ul of the 1kb Gene Ruler ladder (well 1) -Contains DNA ladder, 6X TriTrack DNA, Loading Dye, and Deionized water -Load other wells -Well 2: Control -Well 3: BAC- 15M5 -Well 4: BAC-39K10 -Well 5: BAC-27N17 -Run the gel at 100V for 30 minutes or until the dye front has migrated 2/3 down the gel
- Why is UV light used in Column chromatography experiment ?Describe how you will perform serial dilution. Write and capture your image well.To the right is an image of a dilution that was performed. The volume above the top arrow indicates the volume that should be transferred to the next tube (i.e. Tube 1). The volume listed at the bottom-right of tube 1 indicates the volume of diluent that should be added to that tube. The stock concentration is 50μg/ml and you want to make a solution in tube 1 with a concentration of 0.4μg/ml and a total volume of 3ml. Stock ?ml Tube 1 ?ml a. How many milliliters of stock solution needs to be added to Tube 1? Round your answer to three decimal places (e.g. 0.111). b. How many milliliters of diluent needs to be added to Tube 1? Round your answer to three decimal places.
- What are the underlying physical principles of paper chromatography? What is spectrophotometry, the essence of it?The figure above depicts an agar cube with a side length of 13\, \text{mm}13mm13, start text, m, m, end text. In an experiment, students submerged the cube in red dye for 121212 hours. The red dye permeated 1\, \text{mm}1mm1, start text, m, m, end text on each side, as indicated by the shading in the figure. Volume of a rectangular solid: V = lwhV=lwhV, equals, l, w, h Calculate the volume of the agar cube that remained unpenetrated by the red dye.(The top set of images are photographs of your results for MSA and MAC. The bottom set of images are illustrations that reflect the results you should have observed in the photographs.) Culture #:[Type here] Organism:[Type here] Name: Combination Media: Mystery Organism Identification You will be given a pure culture of one of three organisms. Your assignment is to identify which of the three possibilities is in your culture tube. To do this, you will use two different types of media: Mannitol Salt Agar and MacConkey's Agar. Complete the table below by predicting the reactions of each organism on each medium (Growth or no growth? If you expect growth, what color should it be?). Do this before coming to lab on the due date. The information about each organism below and the information found in the theory sections for the labs should help you make your predictions. If you complete this table correctly, it will be a big help to you while you try to identify MSA MAC your mystery organism!…