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- When acids are added to a solution, the pH should ___________ . a. decrease b. increase c. stay the same d. cannot tell without testing2. Absorbance Data Table Absorbance Concentration 0 mg/dL = blank %3D 0.09 Oi17 0.28 25 mg/dL 50 mg/dL 75 mg/dL 100 mg/dL 035 Unknown solution 6.14 3. Attach a graph of your standard curve. Show the point for the unknown solution on your graph, and determine the concentration of the unknown. Enter this value below (include correct units). Concentration of the unknown solutiononly possible solution DISCUSSION filtration and any other Discuss sterilization technique Autoc auva an.. Filtration Compare each pH measurement 1d be dono ifnULia Why didw add agar +he Any other?
- 0.2 ml of culture . Please explain solutionPlease show the solution. 2.QUESTION 3 An unknown protein was determined using the following method: Bradford method Reagents Stock Bradford reagent: Dissolve 100 mg Coomassie Brilliant Blue G250 in a mixture consisting of 100ml of 85% phosphoric acid, 50ml of 95% ethanol and 50ml 1M NaOH. Store at 4°C until precipitation occurs, at which point it is discarded. Working Bradford reagent: Prepare fresh by diluting 10ml of stock Bradford reagent to 250ml with distilled water. Stock bovine serum albumin (BSA) solution (10mg/ml): Dissolve 0.2g BSA in 20ml distilled water. Working BSA concentration range: 0.5 – 1.25 mg/ml Method 1. Prepare the following test tubes: Table 1: Preparation of test tubes for the Bradford method Blank Tube 1 Tube 2 Tube 3 Tube 4 Tube 5 Unknown Distilled H20 1.0 ml 0.9ml 0.9ml 0.9ml 0.9ml 0.9ml 0.8ml 0.25 mg/ml BSA 0.1 ml 0.5 mg/ml BSA 0.1 ml 0.75 mg/ml BSA 0.1 ml 1.0 mg/ml BSA 0.1 ml 1.25 mg/ml BSA 0.1ml Unknown 0.2ml protein Working 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml Bradford 2.…
- Desired Desired Initial Volume of Stock or Volume of Concentration Volume Concentration Previous Dilution Distilled Water (C2) (V2) (C1) (V1), µL (V2- V1), µL 100 mg/dL 1.2 mL 200 mg/dL 75 mg/dL 1.2 mL 100 mg/dL* 50 mg/dL 1.2 mL 25 mg/dL 1.2 mL2. DECOLORIZATION Dissolve 1g of brown sugar in 15 ml. dist. Water in a small beaker and divide the solution into two equal portions. Place one portion in a test tube and add 1 g boneblack or activated charcoal and gently boil over a small flame for about 2 minutes (replace water lost by evaporation). Allow to cool and filter. How does the filtrate compare with the original solution? What property boneblack is exhibited in this procedure? How do you define this property? 3. CRYSTALLIZATION Mix in a beaker 2g of benzoic acid and 10ml of dist. Water. Boil gently over a flame for about 2 minutes, replacing water lost by evaporation every now and then. Filter the solution while still hot if any residue shows, if none, directly immerse the tube in a beaker of cold water (used ice water if available). Let stay for about 5 min. and observe. Are crystals formed? How do they compare with the original in texture in size of crystals? Define crystallization How can crystallization be carried? 4.…Test solution Indicator thatcan be usedInitial colour ofthe solutionFinal colourof thesolution1. Lemon juice Blue litmus Red
- Table 2. Volume of BSA, protein content, and absorbance readings of reference solutions Solution Volume of BSA standard solution (μL) Protein content(μg/mL) Absorbance value At 595 nm 1 0 0 0 2 10 1 0.022 3 30 3 0.065 4 50 5 0.106 5 70 7 0.178 6 100 10 0.299 7 120 12 0.380 Make a graph by plotting the absorbance values versus the BSA protein content (in μg) for theseven reference solutions. When constructing the graph, be…Part I. For each of the following four questions: calculate and describe how the requested solution would be made. Please show all of your calculations. In all cases the diluent or solvent will be water. Also, assume the smallest volume you can accurately pipette is 1ul. (CaClh: Molecular weight = 111.0 - NaCl: Molecular weight= 58.44) 1. From a 3M CaCh stock solution, you need to make 600 ml of 9 mM CaClh. Indicate how you would make it. 2. You have a 20ul sample of DNA that you want to run in a gel. You are given 5X track dye; how much track dye do you add to your sample so that the track dye concentration in the sample is 1X? 3. You need to make 6 liters of 20% NaCl solution. Indicate how you wvould make it firom powdered NACI.50 mL of 5mM NaCl solution from 1 M NaCl solution