2. a) Calculate the CFU present in 100 mL water sample and b) dilution factor in Tube 5. Consider the following dilution scheme: 5 ml 1 ml 0.5 ml 1 ml 0.1 ml 0.9 ml Water sample 9 ml 4.5 ml 100 ul. 100 ml 45 ml 19 ml 100 ul 100 ul 100 ul 100 pl No. of colonies: TNTC 425 58 12 1 3.
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Q: Calculate the CFU present in 100 mL water sample and b) dilution factor in Tube 5.
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- 2. a) Calculate the CFU present in 100 mL water sample and b) dilution factor in Tube 5. Consider the following dilution scheme: (20 pts.) 5 ml 1 ml 0.5 ml 1 ml 0.1 ml 0.9 ml Water sample 9 mL 4.5 mL 100 μL 100 ml 45 ml 19 ml 100 ul 100 ul 100 μι 100 μL No. of colonies: TNTC 425 58 12 1 3.Calculate the CFU present in 150 mL water sample and b) dilution factor up to 5. Consider the following dilution scheme.An original sample of water containing 4.00 X 106 CFU/mL was diluted by 4 successive 1/10 dilutions. After incubation, 200 colonies were found growing on the plate. How many mLs from the last dilution tube were plated out?
- DILUTION COLONY COUNT CFU/mL 1:106 155000 1:107 15500 1:108 1550 1:109 155 Given these values how would I fill in the rest of this serial dilution table? Also, what would be a 1:1 CFU/mL value based on this table?Serial dilution = previous dilution x dilution 11 mL [2] 3 mL Colony count: 100 0.1 mL sample [1] 9.9 mL 5 mL 0.1 ml. Cokony count Dilution: 10-1 10-3 [3] 150 Dilution factor (DF): [4] [5] [6] Compute for the CFU/mL of the sample. Show your solution and express your final answer to 2 significant figures.Observe the following Plate counts and then determine the correct number of CFU/ml Plate 1 = 564 colonies at 10^-5 dilution Plate 2 = 422 colonies at 10^-6 dilution Plate 3 = 317 colonies at 10^-7 dilution Plate 4 = 93 colonies at 10^-8 dilution 93 x 10^10 CFU/ml 9.3 x 10^-9 CFU/ml 93 x 10^9 CFU/ml 93x 10^8 CFÜ/ml 93x 10^-8 CFU/ml asap please
- 1mL Stock #1 1mL 9mL #2 9mL #3 1mL A 9mL #4 0.1mL 1mL O O B 9mL #5 1mL 1mL 9mL #6 O Using the picture serial dilution scheme and the following information (plate A has 276 colonies, plate B has 298, plate C has 2, and plate D has 30), calculate the average number of colony forming units per mL in the stock tube. Make sure to only use countable plates. Round your answer to the nearest one. Write only the number with any needed commas or decimals. Do not include units. 0.1mL 1mk D O6) 1 mL of supernatant is required for a procedure. The final colored solution proves to be too high to read accurately on the spectrophotometer. 100 μL of supernatant and 900 μL of distilled water are substituted for the original supernatant and the procedure, run as before. The reading from the standard curve is 46 mg/dL. What is the actual amount of substance in the patient serum?Answer the following questions based on Kirby Bauer / MacFarland standards 1-0.5 McFarland standard solution is ideally used for inoculation of plated for Kirby Bauer test. a)True b)False Principle of Kirby Bauer test is the diffusion of antibiotic agents in to Agar based media. a) True b) False
- Andrew has to prepare 30 plates (use maximum volume), 15 slants (small test tube), and 15 stabs (big test tube) of Luria Bertani Agar. a. What is the total volume of the medium needed? b. What is the amount of each component needed, given the following media composition per liter of distilled water:3) With our 12.5 ug/mL solution, we will make serial dilutions with a dilution factor of 2. We want to make 1 mL of 1/2, 1/4, 1/8, 1/16, 1/32, and 1/64 dilutions. Draw a diagram describing how you will conduct the serial dilutions and insert it below. The 1/2 dilution (tube 2) will be made from the 12.5 ug/mL solution (tube 1). The 1/4 dilution (tube 3) will be made from the 1/2 dilution (tube 2LWhat is the minimal concentration (MIC) for disinfectant number 1? Just write the corresponding number including the decimal as written. ug/mL. Disinfectant 1 2 3 4 concentration 0.1 ug/mL +++ ++++ ++ +++ 0.2 ug/mL ++ ++ + ++ 0.5 ug/mL + + 1.0 ug/mL 5.0 ug/mL subculture 1 2 3 4 0.1 ug/mL ++++ ++++ ++++ ++++ 0.2 ug/mL ++++ ++++ ++++ ++++ 0.5 ug/mL ++++ ++++ ++++ 1.0 ug/mL ++++ 5.0 ug/mL Submit response