The UWC Biotechnoloclass undertook a study to determine the microbiological quality ofmince-meat samples that were obtained from four local retail outlets. You have performed thefollowing serial dilution of the mince samples. Firstly, 25 g of the minced samples was transferredto 225 ml 4 strength Ringers'-solution, followed by two (2) 10-fold dilutions and finally three (3)100-fold dilutions. These dilutions were then pour-plated out (1000 µl of each) onto the requiredmedium. The colony forming units (cfu) for the Enterobacteriaceae count is listed in the tablebelow:1346.Total Viable Count>300>300>300274283>300>300>300286293Mold and YeastCount>300>300129142>300>300127131StaphylococcalCount>300168183101>300156162Enterobacteriaceae>300>30026427Count>300>300248252a.Determine the log colony forming units per gram of product for the differentcounts.b. If the allowable microbiological standard for the Enterobacteriaceae count is 103cfu/g, what can you conclude about the overall microbiological standard of theproduct. [NB - Good or bad will not suffice. You need to provide moreexplanations for your counts and give recommendations where appropriate]

In-order to calculate the amount of bacteria present in a highly concentrated stock solution ( a…

The UWC Biotechnolo class undertook a study to determine the microbiological quality of mince-meat samples that were obtained from four local retail outlets. You have performed the following serial dilution of the mince samples. Firstly, 25 g of the minced samples was transferred to 225 ml 4 strength Ringers'-solution, followed by two (2) 10-fold dilutions and finally three (3) 100-fold dilutions. These dilutions were then pour-plated out (1000 µl of each) onto the required medium. The colony forming units (cfu) for the Enterobacteriaceae count is listed in the table below: 2 3 4 5 6. Total Viable Count >300 >300 >300 274 28 3 >300 >300 >300 286 29 3 Mold and Yeast >300 >300 129 14 Count >300 >300 127 13 1 Staphylococcal Count >300 168 18 3 10 1 >300 156 16 2 Enterobacteriaceae >300 >300 264 27 3 Count >300 >300 248 25 a. Determine the log colony forming units per gram of product for the different counts. b. If the allowable microbiological standard for the Enterobacteriaceae count is 10 cfu/g, what can you conclude about the overall microbiological standard of the product. [NB - Good or bad will not suffice. You need to provide more explanations for your counts and give recommendations where appropriate]

The UWC Biotechnolo class undertook a study to determine the microbiological quality of mince-meat samples that were obtained from four local retail outlets. You have performed the following serial dilution of the mince samples. Firstly, 25 g of the minced samples was transferred to 225 ml 4 strength Ringers'-solution, followed by two (2) 10-fold dilutions and finally three (3) 100-fold dilutions. These dilutions were then pour-plated out (1000 µl of each) onto the required medium. The colony forming units (cfu) for the Enterobacteriaceae count is listed in the table below: 2 3 4 5 6. Total Viable Count >300 >300 >300 274 28 3 >300 >300 >300 286 29 3 Mold and Yeast >300 >300 129 14 Count >300 >300 127 13 1 Staphylococcal Count >300 168 18 3 10 1 >300 156 16 2 Enterobacteriaceae >300 >300 264 27 3 Count >300 >300 248 25 a. Determine the log colony forming units per gram of product for the different counts. b. If the allowable microbiological standard for the Enterobacteriaceae count is 10 cfu/g, what can you conclude about the overall microbiological standard of the product. [NB - Good or bad will not suffice. You need to provide more explanations for your counts and give recommendations where appropriate]

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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