You were tasked to check the quality of your E.coli stock from your -80 °C freezer. This isolate will be sent in DUPLICATES to THREE different laboratories using LB slants . You need to revive the sample in 1 tube of LB broth and inoculate it to 1 Eosin Methylene Blue plate for quality control. From the same LB broth, you need to take a sample and inoculate it into the LB slants that you are going to send. Using a flow diagram, detail the steps and processes (media preparation, sterilization, inoculation) that you need to do. Show your calculations, aseptic techniques involved, principles, and expected results. Use the product specifications of the culture media manufactured by HiMedia or other culture media manufacturer to guide you in your media prep.

You were tasked to check the quality of your E.coli stock from your -80 °C freezer. This isolate will be sent in DUPLICATES to THREE different laboratories using LB slants . You need to revive the sample in 1 tube of LB broth and inoculate it to 1 Eosin Methylene Blue plate for quality control. From the same LB broth, you need to take a sample and inoculate it into the LB slants that you are going to send. Using a flow diagram, detail the steps and processes (media preparation, sterilization, inoculation) that you need to do. Show your calculations, aseptic techniques involved, principles, and expected results. Use the product specifications of the culture media manufactured by HiMedia or other culture media manufacturer to guide you in your media prep.

Aquaculture Science

3rd Edition

ISBN:9781133558347

Author:Parker

Publisher:Parker

Chapter2: Aquatic Plants And Animals

Section: Chapter Questions

Problem 2KA

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