1.why is it not advisable to move/touch the agarose gel in the process of hardening 2.what is the use or function of the TAE buffer that is poured or found in the gel box 3.how can you tell if agel is running

You are transferring a bacterial culture from a tube of broth to a Petri dish containing trypticase soy agar. Please list the steps that are necessary to make the transfer successfully and with minimal risk. of contamination.

1a.) How pH of the culture medium affects the cell culture? Please give examples of low pH and high-pH culture conditions.   1b.) Describe the difference between defined and undefined cell culture media. What are the advantages and disadvantages of both of them?

The following chart contains four facets. Each shows the amount of a substance in different conditions: • when the cells are adhering to a surface • when the cells are growing in suspension for different amounts of time There are 1 different glyphs shown in the frames. suspension growth suspension growth 1433B 1433E 6.000% 4.000% 16.000% 10.666% 2.000% 5.333% 0.000% 0.000% 1433G 1433Z 4.500% 6.000% 3.000% 4.000% 1.500% 2.000% 0.000% 0.000% adhesion growth O min * 10 min 30 min 60 min growth O min uoisaupe 10 min 30 min 60 min

Answer the following questions briefly and concisely 1.How do bacteria in a chemostat and those in a batch culture vary from one another? 2.  What happens in a chemostat if the dilution rate is higher than the organism's maximum specific growth rate? 3.Does a chemostat require the use of pure cultures? 4. Why would a complicated culture media for Leuconostoc mesenteroides be simpler to make than one with a fixed chemical composition?

using sample prep aka the protocol use it to fill in the tables

Please help me with this question. The answer choices are in [ ] If bacteria are killed by an antibiotic, the antibiotic is       ["Bactericidal" OR "Bacteriostatic"]  and we would expect       ["Growth" OR "No Growth"]  on our TSA subculture plates If an antibiotic does not kill the bacteria, but rather the bacterial growth is inhibited, the antibiotic is       ["Bactericidal" OR "Bacteriostatic"]  .  For these types of antibiotics, we would expect       ["Growth" OR "No Growth"]  on our TSA subculture plates in this latter type of antibiotic.

Explain how to prepare 250 ml liquid and 500 ml solid form separately from LB (Luria Bertani) medium whose components and concentrations are given below. Yeast extract 5 g/L Peptone 10 g/L NaCl 10 g/L Agar 1.5 g/L

use these OD numbers to plot growth curve for E. coli K12- and estimate generation time for this culture. Table 1. Absorbance (O.D.600) measured for growing culture of E. coli K12 (time course) Incubation time O.D.600 Incubation time O.D.600 O min 3 hrs 3 hrs 30 min 4 hrs 4 hrs 30 min 5 hrs 5 hrs 30 min 0.11 1.75 30 min 0.20 1.94 1 hr 1 hr 30 min 2 hrs 2 hrs 30 min 0.27 2.24 0.51 2.48 2.31 2.19 0.65 1.30

What is the result of this biochemical test in the gelatin deep?  Which enzyme is this testing for? What tool is used to inoculate this medium? Gelatin positive liquid, use needle, protease,