You obtain a 1 mL sample of contaminated drinking water in order to calculate the number of  fecal bacteria in the water. The 1 mL sample is diluted into a first bottle with 99 mL of sterile  saline. One mL from the first bottle is transferred into a second bottle of saline containing 9  mL. Finally, 1 mL from the second bottle is transferred to a third bottle with 9 mL of saline.  You create two spread plates from the third bottle: plating 0.1 mL results in 106 colonies,  while plating 1 mL results in 562 colonies. How many fecal bacteria per mL are in the drinking  water?    This is a homework assignment, and I am struggling to do this correctly. Thanks for your help.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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You obtain a 1 mL sample of contaminated drinking water in order to calculate the number of 
fecal bacteria in the water. The 1 mL sample is diluted into a first bottle with 99 mL of sterile 
saline. One mL from the first bottle is transferred into a second bottle of saline containing 9 
mL. Finally, 1 mL from the second bottle is transferred to a third bottle with 9 mL of saline. 
You create two spread plates from the third bottle: plating 0.1 mL results in 106 colonies, 
while plating 1 mL results in 562 colonies. How many fecal bacteria per mL are in the drinking 
water? 

 

This is a homework assignment, and I am struggling to do this correctly. Thanks for your help.

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