Which would you choose if you needed to make a partial diploid for complementation analysis? rolling circle plasmid relaxed plasmid O Hfr strain O prime factor

I need help putting these topics back in order. Could my answers be checked to see if i have them in the correct order?Based on my work from the first image, what would the correct order of topics be for the next image?

After their marriage, Calle will be covered by her husbands health insurance. Calle is ethical in completing her application for this coverage and mentions MS diagnosis. But she has question. Where could Calle get information as to whether or not the insurance company will cover her expensive medications for this disease

My question is     The feeling of pain is initiated by three types of __________________ that are localized on the terminals of free nerve endings