Place the steps of the double-strand break model of recombination in order. DNA polymerase fills in gaps in the DNA exonuclease generates 3' single-stranded overhangs DNA ligase seals the nicked DNA First step Last step Answer Bank homologous region of intact homolog is invaded by free end endonuclease cleaves both strands of one homolog

Which one is the correct one?

DNA polymerase occasionally incorporates the wrong nucleotide during DNA replication. If left unrepaired, the base-pair mismatch that results will lead to mutation in the next replication. As part of a template strand, the incorporated wrong base will direct the incorporation of a base complementary to itself, so the bases on both strands of the DNA at that position will now be different from what they were before the mismatch event. The MER-minus strain of yeast does not have a functional  mismatch excision repair system, but it has normal base excision repair and nucleotide excision repair systems. Which of the following statements is correct about differences in the mutation spectrum between MER-minus and wildtype yeast? More than one answer is correct. Options:   More point mutations will arise in MER-minus yeast.   Fewer point mutations will arise in MER-minus yeast as compared with wildtype.   Of the total point mutations that…

Draw the steps that take place during conjugation.

Which of the following DNA-synthesizing enzymes has 5’ to 3’ polymerization activity? Mark all the answers that apply.   DNA polymerase I DNA polymerase III DNA ligase Telomerase Primase

Write the order of the following components for the process of Homologous Recombination repair: A. DNA synthesis B. Strand invasion C. Double-strand break D. nucleases create uneven strands E. DNA ligation

Which elements are required for DNA to behave as a chromosome in yeast cells: a centromere an autonomous replicating sequence two telomeres Antibiotic selection marker

Inability to recognize double stranded DNA breaks results in increases rates of:   Question 20 options:   translocations   fragmented chromosomes   increased rates of nonhomologous end joining   gene deletions   increased rates of homology directed repair