The same restriction digestion experiment was repeated for EcoR1 on the same DNAmolecule. However, the incubation period of the reaction was lengthened for an additional 2hours due to some unforeseen circumstances. Illustrate below the results you would observeafter gel electrophoresis and explain your findings.

Answered: The same restriction digestion…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

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The same restriction digestion experiment was repeated for EcoR1 on the same DNA
molecule. However, the incubation period of the reaction was lengthened for an additional 2
hours due to some unforeseen circumstances. Illustrate below the results you would observe
after gel electrophoresis and explain your findings.

Expert Solution

Step 1

It is given that the incubation period of the restriction digestion with EcoRI on the same DNA was lengthened for an additional two hours due to unforeseen circumstances.

In this case, the reaction digestion pattern will differ because of over-digestion and the star activity of the enzyme. When the enzymes are kept for digestion for a long time, they will start cutting at non-specific sites on the DNA resulting in the formation of unexpected DNA fragments, and they will appear as smears after running on the agarose gel.

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