molecular scissors", justify that

Q: The same restriction digestion experiment was repeated for EcoR1 on the same DNA molecule. However,…

A: It is given that the incubation period of the restriction digestion with EcoRI on the same DNA was…

Q: The type of restriction enzymes that do not usually require ATP is : * О Туре ! О Туре I О Туре II O…

A: A restriction enzyme, also termed as a restriction endonuclease or restrictase, is an enzyme that…

Q: Why is the restriction enzyme digestion step critical in 3C assays?

A: Genetic information is stored in DNA and we often consider the DNA molecule only as a linear…

Q: What is the principle of the Restriction Fragment Length Polymorphism (RFLP) in the diagnosis of…

A: Restriction fragment length polymorphism (RFLP) : it is a method used in genome mapping and…

Q: What is a restriction enzyme? What structure does it recognize?What type of chemical bond does it…

A: Werner Arber, a Swiss microbiologist, winner of 1978 Nobel Prize in Physiology, for his discovery…

Q: Draw an Illustration of the steps in restriction digestion and PCR [ Please make it clean and…

A: Restriction digestion is the process of cutting the DNA sequence at a particular nucleotide sequence…

Q: Using the restriction site for SalI, suggest another restriction enzyme that would cut at the same…

A: Isoschizomers are a set of enzymes that share the same recognition sequence in the restriction…

Q: 1. Restriction enzymes can occasionally cut at site(s) other than its restriction site. 2. DNA…

A: NOTE:- As you have posted multiple questions, we will solve first two parts for you, To get the…

Q: Statement 1: Restriction enzymes are sequence-specific, which means that they only recognize a short…

A: Restriction endonuclease is an important tool in DNA Recombinant technology or genetic engineering.…

Q: Match the term with its description.

A: There are various enzymes and gene components that are studied in techniques of genetic engineering.…

Q: Fill in the blank spaces by selecting the correct enzyme for each of the restriction enzyme…

A: DNA  is cleaved to form DNA fragments with the help of an enzyme known as the restriction enzyme.…

Q: An effective DNA probe can sometimes be developed by knowing the amino acid sequence of the protein…

A: A segment of DNA to be cloned is known as probe. It could be radioactive in gel electrophoresis

Q: Define restriction endonuclease and explain what it does.

A: Restriction endonuclease also known as Restriction enzyme, is a protein produced by bacteria that…

Q: Restriction digestion of DNA fragments is not sequence specific. True False

A: Within a few years after discovering EcoB, EcoK, and HindII, scientists were already experimenting…

Q: Why does Northern blotting not require the use of restriction enzymes?

A: Restriction enzyme naturally cleaves DNA because it's have double stranded sequence of neucleiotides…

Q: Using the chart posted to the left of the microwave in lab, what is the 3. restriction recognition…

A: Step 1 In 1963, Arber discovered that Escherichia coli is able to protect itself from the attack of…

Q: What is the minimum incubation time of the restriction enzyme in the experiment?

A: Restriction enzymes are found in bacteria as a defense against invading bacteriophages. Restriction…

Q: Draw a diagram showing what pGEM will look like after it has been digested with BamHI.

A: BamHI: BamHI is a restriction enzyme obtained from Bacillus amyloliquefaciens that specifically cuts…

Q: Which of these statements is INCORRECT about restriction enzymes

A: A restriction enzyme is a protein isolated from bacteria. It help to  cleaves DNA sequences at…

Q: True or False: Restriction enzymes normally prevent viral DNA or RNA entry in bacteria.

A: A bacterium utilizes a restriction protein to defend against bacterial infections called…

Q: Restriction Enzymes with LongerRecognition Sites Produce Larger _______ Fragments

A: The restriction enzyme is the molecular scissors that cut at a specific site. These are naturally…

Q: Identify the basic steps (in right order) in AFLP marker analysis. Explain briefly how this marker…

A: Basic steps in AFLP marker analysis.

Q: You want to insert a sequence in the lacI gene to alter its function,what restriction enzyme(s) will…

A: The lacI gene is a part of the lac operon, that codes for a protein that inhibits the expression of…

Q: why the bacteria synthesize Restriction endonucleases enzymes. What’s the main purpose and how the…

A: Restriction endonucleases (RE) are bacterial enzymes that are used to clip off DNA crosswise at…

Q: Calculate the average sizes and numbers of DNAfragments produced by digesting human genomic DNAwith…

A: DNA is the genetic material of many eukaryotic and prokaryotic organisms. It is a double-stranded…

Q: All of the following are true about Restriction endonucleases except fashion

A: DNA fragments are cleaved at particular recognition sites by some enzymes known as Restriction…

Q: N

A: Recombinant DNA , molecules of DNA from two different species that are inserted into a host organism…

Q: When using blunt-end primers, how would you determine the correct plasmid construct from the…

A: Ligation is the joining or ligation of two nucleic acid fragments through the action of a ligase…

Q: What feature is commonly seen in the sequences recognized by type II restriction enzymes?

A: Introduction  Enzymes are the crucial biomolecules which assists in various biochemical reactions…

Q: Consider the following:  Restriction enzymes bind and cut between bases T and A.  Based on this,…

A: VNTR  stands for Variable Number of Tandem Repeats, means the DNA sequence (Satellite DNA) repeats…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.