Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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Restriction enzymes are referred to as "molecular scissors", justify that
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- A DNA fingerprint consists of sections of DNA that have been cut with restriction endonuclease enzymes and probed to display the DNA fragments, or restriction digests, of interest.arrow_forwardDr. Doom has a DNA sequence of 2000 bp. Enzyme X and Enzyme Y restriction sites and their positions are indicated here. Recall that Enzyme X is methylation sensitive, while Enzyme Y is not. 200 O 100 1800 2000 bp 1000 1500 Enzyme Y Enzyme Y Enzyme X Enzyme Y Enzyme X If this gene is methylated, which of the following combinations of DNA fragments would you expect from this restriction digest? O 5 bands = 1O bp, 200 bp, 1000 bp. 1500 bp, and 1800 bp O 3 bands = 200 bp, 500 bp, and 1300 bp %3D O No bands = DNA will be completely digested %3D O 4 bands = 100 bp. 200 bp, 800 bp, and 900 bparrow_forwardThe polymerase chain reaction (PCR) is used by scientists to amplify DNA, particularly when the quantity of DNA is very small, mixed, or contaminated with other organisms. Explain (with words) the how PCR works using a diagram to help illustrate this (show a minimum of three cycles to illustrate your point).arrow_forward
- How does that length compare to that of restriction enzymes? Implications?arrow_forwardRestriction endonucleases are bacterial enzymes that cleave duplex (double-stranded) DNA at specific nucleotide sequences. The mode of replication of the animal virus SV40 has been investigated by using restriction endonucleases that cleave SV40 DNA into a number of unique segments. Like most viruses, SV40 DNA is circular. The map positions of the 11 fragments produced by a pair of restriction endonucleases are shown on the next page. Immediately following a 5 or 10 minute pulse of radioactively labeled thymidine, labeled SV40 molecules that have completed replication during the pulse are isolated. These newly replicated DNA molecules are digested by the restriction endonucleases and the resulting fragments are analyzed for the relative amounts of pulse label they contain. The results are in the table below. Assume that at the time the label was added there was a random population of replicating SV40 DNA molecules in all possible stages of synthesis. From the information given below,…arrow_forward
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