Choose one product of recominbinant DNA technology and write the steps involved in the production of the modified organism. I chose Flavr Savr Tomato. However, I can't find the steps involved for this. Thank you for your help.   Ps. Please make it simple and easy to understand. If you can give me a simplified version of a certain source. That will be a big help :)

Briefly outline the steps of DNA extraction as carried out in a lab for the purposes of sequencing, with reference to a published protocol from a company that supplies regents and kits for DNA extraction (e.g Qiagen, ThermoFischer, Invitrogen, or similar). You should note the refinements compared to the protocol used in your practical session.

When performing cloning experiments, it is not always necessary to treat sources of DNA with the same restriction enzyme. For example, DNA treated with EcoRI can be combined with DNA from a treatment using FunII. Explain why this is possible.

What is CRISPR? Give two applications for this technology.

Please describe the detailed procedures of southern blot. Explain the protocol and principle of restriction fragment length polymorphism (RFLP) and how RFLP is used for genetic disease diagnosis.

Explain why the 16S rRNA gene sequencing is suitable for bacterial identification in general and why it is mostly limited to genus level.

The current state-of-the-art in forensic DNA profiling involves the PCR-amplification and analysis ofshort tandem repeats, STRs, in the human genome. This approach has many distinct advantages.Please list and explain three of those advantages.

Use this information to match the list of probes to the 3 samples below: You have isolated several DNA sequences from a variety of mouse tissues. You have labeled each one of them with a radioisotope and will use them as probes on blots of several DNA and RNA somples. Below are a list of all the probes you generated (probes A through E) and a list of all the DNA and RNA samples that you will analyze (Samples 1 through 3) Beside each sample, write the letters corresponding to oll the probes that will bind to a complementary sequence in that sample. These responses are graded all or nothing! List of probes Probe A: promoter sequence of a gene that is only expressed in the nervous system Probe B: promoter sequence of one of the genes encoding a ubiquitously expressed histone protein Probe C: coding sequence of a gene that is only expressed in the nervous system Probe D: coding sequence of one of the genes encoding a ubiquitously expressed histone protein Probe E: intron of a gene that is…

You need to complete the following steps for an experiment. Explain whether an acrylamide gel or an agarose gel is more appropriate to use for the following experiments. Purifying tRNA from total RNA Isolating a vector insert for cloning Analyzing PCR products

Describe several applications of DNA profiling and microarrayanalysis.