Sickle cell anaemia is caused by a mutation in the HBB gene. The normal wild-type βA allele contains an MstII restriction site; in the mutated sickle-cell βS allele this restriction site has been lost. PCR amplification of part of the gene encompassing the mutation site from a normal unaffected individual results in a product of 110bp. Digestion of the PCR product with MstII and subsequent gel electrophoresis results in a single band of 55bp.   How many bands and of what size would you expect to see in individuals who: i) has sickle-cell anaemia (has two βS alleles) ii) carry the disease (sickle cell trait) (has one βS and one βA allele)   a. Individual Number of bands Size of bands (bp) Sickle-cell anaemia (has two βS alleles) 2 55 Sickle cell trait) (has one βS and one βA allele) 1 110   b. Individual Number of bands Size of bands (bp) Sickle-cell anaemia (has two βS alleles) 2 110 Sickle cell trait) (has one βS and one βA allele) 2 55 & 110   c. Individual Number of bands Size of bands (bp) Sickle-cell anaemia (has two βS alleles) 1 110 Sickle cell trait) (has one βS and one βA allele) 1 110   d. Individual Number of bands Size of bands (bp) Sickle-cell anaemia (has two βS alleles) 1 110 Sickle cell trait) (has one βS and one βA allele) 2 55 & 110

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
Topic Video
Question

Sickle cell anaemia is caused by a mutation in the HBB gene. The normal wild-type βA allele contains an MstII restriction site; in the mutated sickle-cell βS allele this restriction site has been lost.
PCR amplification of part of the gene encompassing the mutation site from a normal unaffected individual results in a product of 110bp. Digestion of the PCR product with MstII and subsequent gel electrophoresis results in a single band of 55bp.

 

How many bands and of what size would you expect to see in individuals who:

i) has sickle-cell anaemia (has two βS alleles)

ii) carry the disease (sickle cell trait) (has one βS and one βA allele)

  a.
Individual Number of bands Size of bands (bp)

Sickle-cell anaemia
(has two βS alleles)

2 55
Sickle cell trait)
(has one βS and one βA allele)
1 110
  b.
Individual Number of bands Size of bands (bp)

Sickle-cell anaemia
(has two βS alleles)

2 110
Sickle cell trait)
(has one βS and one βA allele)
2 55 & 110
  c.
Individual Number of bands Size of bands (bp)

Sickle-cell anaemia
(has two βS alleles)

1 110
Sickle cell trait)
(has one βS and one βA allele)
1 110
  d.
Individual Number of bands Size of bands (bp)

Sickle-cell anaemia
(has two βS alleles)

1 110
Sickle cell trait)
(has one βS and one βA allele)
2 55 & 110
Expert Solution
steps

Step by step

Solved in 2 steps

Blurred answer
Knowledge Booster
Patterns of inheritance
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON