Sickle cell anaemia is caused by a mutation in the HBB gene. The normal wild-type βA allele contains an MstII restriction site; in the mutated sickle-cell βS allele this restriction site has been lost. PCR amplification of part of the gene encompassing the mutation site from a normal unaffected individual results in a product of 110bp. Digestion of the PCR product with MstII and subsequent gel electrophoresis results in a single band of 55bp.   How many bands and of what size would you expect to see in individuals who: i) has sickle-cell anaemia (has two βS alleles) ii) carry the disease (sickle cell trait) (has one βS and one βA allele)   a. Individual Number of bands Size of bands (bp) Sickle-cell anaemia (has two βS alleles) 2 55 Sickle cell trait) (has one βS and one βA allele) 1 110   b. Individual Number of bands Size of bands (bp) Sickle-cell anaemia (has two βS alleles) 2 110 Sickle cell trait) (has one βS and one βA allele) 2 55 & 110   c. Individual Number of bands Size of bands (bp) Sickle-cell anaemia (has two βS alleles) 1 110 Sickle cell trait) (has one βS and one βA allele) 1 110   d. Individual Number of bands Size of bands (bp) Sickle-cell anaemia (has two βS alleles) 1 110 Sickle cell trait) (has one βS and one βA allele) 2 55 & 110

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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Sickle cell anaemia is caused by a mutation in the HBB gene. The normal wild-type βA allele contains an MstII restriction site; in the mutated sickle-cell βS allele this restriction site has been lost.
PCR amplification of part of the gene encompassing the mutation site from a normal unaffected individual results in a product of 110bp. Digestion of the PCR product with MstII and subsequent gel electrophoresis results in a single band of 55bp.

 

How many bands and of what size would you expect to see in individuals who:

i) has sickle-cell anaemia (has two βS alleles)

ii) carry the disease (sickle cell trait) (has one βS and one βA allele)

  a.
Individual Number of bands Size of bands (bp)

Sickle-cell anaemia
(has two βS alleles)

2 55
Sickle cell trait)
(has one βS and one βA allele)
1 110
  b.
Individual Number of bands Size of bands (bp)

Sickle-cell anaemia
(has two βS alleles)

2 110
Sickle cell trait)
(has one βS and one βA allele)
2 55 & 110
  c.
Individual Number of bands Size of bands (bp)

Sickle-cell anaemia
(has two βS alleles)

1 110
Sickle cell trait)
(has one βS and one βA allele)
1 110
  d.
Individual Number of bands Size of bands (bp)

Sickle-cell anaemia
(has two βS alleles)

1 110
Sickle cell trait)
(has one βS and one βA allele)
2 55 & 110
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