Sickle cell anaemia is caused by a mutation in the HBB gene. The normal wild-type βA allele contains an MstII restriction site; in the mutated sickle-cell βS allele this restriction site has been lost. PCR amplification of part of the gene encompassing the mutation site from a normal unaffected individual results in a product of 110bp. Digestion of the PCR product with MstII and subsequent gel electrophoresis results in a single band of 55bp. How many bands and of what size would you expect to see in individuals who: i) has sickle-cell anaemia (has two βS alleles) ii) carry the disease (sickle cell trait) (has one βS and one βA allele) a. Individual Number of bands Size of bands (bp) Sickle-cell anaemia (has two βS alleles) 2 55 Sickle cell trait) (has one βS and one βA allele) 1 110 b. Individual Number of bands Size of bands (bp) Sickle-cell anaemia (has two βS alleles) 2 110 Sickle cell trait) (has one βS and one βA allele) 2 55 & 110 c. Individual Number of bands Size of bands (bp) Sickle-cell anaemia (has two βS alleles) 1 110 Sickle cell trait) (has one βS and one βA allele) 1 110 d. Individual Number of bands Size of bands (bp) Sickle-cell anaemia (has two βS alleles) 1 110 Sickle cell trait) (has one βS and one βA allele) 2 55 & 110
Sickle cell anaemia is caused by a mutation in the HBB gene. The normal wild-type βA allele contains an MstII restriction site; in the mutated sickle-cell βS allele this restriction site has been lost.
PCR amplification of part of the gene encompassing the mutation site from a normal unaffected individual results in a product of 110bp. Digestion of the PCR product with MstII and subsequent gel electrophoresis results in a single band of 55bp.
How many bands and of what size would you expect to see in individuals who:
i) has sickle-cell anaemia (has two βS alleles)
ii) carry the disease (sickle cell trait) (has one βS and one βA allele)
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