The BamH1 enzyme comes at a concentration of 100,000 U/ml. You are asked to digest 20 ug of DNA with this enzyme. Determine: a) How many units will you need? b) How will you dispense them?
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
The BamH1 enzyme comes at a concentration of 100,000 U/ml. You are asked to digest 20 ug of DNA with this enzyme.
Determine:
a) How many units will you need?
b) How will you dispense them?
The term "specific activity" describes the amount of an enzyme's enzymatic activity that is produced in units per milligram (U/mg) of protein concentration. It measures how well an enzyme catalyzes a certain process. A higher specific activity suggests better enzymatic purity and efficiency since less of the enzyme is required to accomplish a given reaction rate. A key factor in the techniques used to characterize and purify enzymes is specific activity.
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