The BamH1 enzyme comes at a concentration of 100,000 U/ml. You are asked to digest 20 ug of DNA with this enzyme. Determine: a) How many units will you need? b) How will you dispense them?

Cystic fibrosis (CF) is an inherited disorder caused by different types of mutations, many of which prevent ions from moving across cell membranes. Normally there are channel proteins that allow passage of the ions, but in patients with one kind of CF these proteins seem odd. Closer examination shows that these proteins display the correct amino acid sequence. However, they fail to do their job. A) Given that the primary structure of the protein is correct, what can you infer about the DNA sequence for the gene coding this protein on this patient, is there a mutation? Explain. B) Why is the primary structure insufficient to guarantee the proper function of the protein?

Which of the following amino acids is unique in that it can bind both non-specifically to the DNA backbone as well as to specific DNA bases? a) Lysine  b) Asparagine c) Arginine  d) Phenylalanine e) Leucine

How much 340bp insert DNA should be added to a ligation in which 50ng of 4.3kb vector will be used? The desired vector: insert molar ratio needs to be 1:3.

After running our protein samples on an SDS-PAGE gel, we will use a Coomassie-based dye (Gel Code Blue) to stain the gel. Based on your prior experience with Coomassie dye and understanding of its function, what do you predict this dye to be able to stain? A) Only protein tags B) Only DHFR protein C) Only recombinant proteins D) All proteins

1) Prepare the following enzymatic reaction, present it in tabulated form. In a final volume of 30 ul, where buffer 4 (10 ml). How much volume of each reagent would be used and how much of water? Is there any problem? 2) The DNA pol 1 enzyme comes at a concentration of 50,000 U/ml. You have to prepare a 50 ug PCR reaction where you must use 0.05 U/ml reaction. You add 10 ul of PCR buffer, 2 ng of tempered DNA that is at a concentration of 0.5 ng/ul, primers (which are at 200 mM) so that each one remains at a concentration of 200 uM, Mg+2 that is 5 mM (10 X), enzyme and water. Present the table of all the reagents included in the reaction, the volumes of each one in ul. Present where the initial and final concentration of each reagent applies. Assume you have micropipettes for all values.

1. A) Explain this statement, “Most enzymes are gene-encoded proteins, but most enzymes are not only protein". B) You recently isolated a new enzyme in your lab, and you named it "lipidase 1120". As a temporary name, this is fine. Who is responsible for assigning a formal name, and on what criteria will that name be assigned?

At higher amounts of protein, the Bradford assay is not linear. Consider the plot to the right: what is the maximum amount of protein a sample could contain and still fall within a standard curve? Briefly explain your reasoning.

Choose any two of the topics (experiments) outlined below. Your response should include all of the subheadings mentioned below and also abide by the instructions provided. 1) The Phoebaus A Levene experiment: DNA is a polymer. 2) Poly-U experiment by Marshall Nirenberg and J. Heinrich Matthaei, 1961. 3) Kornberg experiment: Fidelity (accuracy) of synthesis. 4) The Fraenkel-Conrat virus experiment. 5) Meselson and Stahl mode of the DNA replication experiment. 6) The Hammerling experiment: Cells store hereditary information in the nucleus. 7) The transplantation experiment: Each cell contains a full set of genetic instruction. SUBHEADINGS 1. Introduction/Study Problem Introduction. Report on what has been done before and identify the gaps that have arisen from your research question. 2. Hypothesis [the proposed explanation made on the basis of limited evidence as a starting point for further investigations. 3. Possible research question 4. Objectives of the experiment 5. Methodology 6.…

A scientist is researching GS1, an enzyme with a relative molecular mass (Mr) of 78,000 present in a bacterium. The scientist has isolated two mutant strains of the bacterium as described below. Strain A: In this strain the GS1 protein is completely non-functional. Analysis of strain A shows that it produces a shortened GS1 protein with an Mr of only 38,000. Strain B: This produces functional GS1, but the Kcat is somewhat reduced. Analysis shows it produces a lengthened form of GS1, with an Mr of about 86,000. What type(s) of mutation may have occurred in the GS1 gene in strain A?