If your DNA extraction were a concentration of 45ng DNA/ug, how would you prepare 500 ul of a 1 ng/ul solution of DNA in water?

After running our protein samples on an SDS-PAGE gel, we will use a Coomassie-based dye (Gel Code Blue) to stain the gel. Based on your prior experience with Coomassie dye and understanding of its function, what do you predict this dye to be able to stain? A) Only protein tags B) Only DHFR protein C) Only recombinant proteins D) All proteins

You gathered your data from Lab 4, and found the following values: Total Activity of lysozyme in HEW: 14 units Total Activity of lysozyme in carb 1: 21 units Total amount of protein in HEW: 70 mg Total amount of protein in Carb 1: 15 mg Calculate the extent of purification of lysozyme in Carb 1.       150%     7 fold     70%     15 fold

H2N 1.) Look carefully at this nucleotide: N: N- Но-Р-О OH a.) Number the carbons in the sugar group. (Remember the "prime" symbols.) b.) Is this a purine or a pyrimidine? How do you know? c.) Would this nucleotide be used for DNA or RNA? How do you know? (Be specific.) d.) Is this nucleotide ready to be used for DNA replication or RNA transcription? Why/why not? e.) If this nucleotide were incorporated into a growing DNA or RNA strand, where would the next added nucleotide be attached to this one?

Give detailed Solution with explanation needed..please explain (don't copy the answer any where

Please describe the protein purification process with the aim of purifying a protein which locates on nucleus membrane.

A number of techniques can separate proteins on the basis of their differences in mass. Describe the use of two of these techniques, centrifugation and gel electrophoresis. The blood proteins transferrin (MW 76 kDa) and lysozyme (MW 15 kDa) can be separated by rate zonal centrifugation or SDS-polyacrylamide gel electrophoresis. Which of the two proteins will sediment faster during centrifugation? Which will migrate faster during electrophoresis?

You add 1 ul of EcoRI into 50 ul of your reaction of which contains 10 ug of DNA. How long would it take to completely digest the DNA?

The following proteins were separated by SDS-PAGE in the presence of mercaptoethanol. Sketch the relative positions of the various polypeptides on the gel. Label the positive and negative ends of the gel.Protein A: 40 kDa single polypeptideProtein B: 80 kDa protein, made up of two subunits of molecular weight 20 kDa and 60 kDa, held together by noncovalent interactionsProtein C: 200 kDa protein, made up of four identical subunits (50 kDa each) linked together by disulfide bonds

We run the HinDIII-digest of lambda-DNA as the standard ladder on the gel.Number the largest fragment as band #1, the next largest as band #2, etc. How would the slowest band from the HinDIII-digest of pGLO align with the the bands of the ladder? 1) Even with band #1.   2) Even with band #2.   3) Even with band #3.   4) Even with band #4.   5) Even with band #5.   6) Even with band #6.   7)Even with band #7.   8) Between bands #1 and #2,   9) Between bands #2 and #3,   10) Between bands #3 and #4,   11)Between bands #4 and #5,   12) Between bands #5 and #6,   13) Between bands #6 and #7,