Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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How much buffer would you need to dilute a stock solution of DNA that is at a concentration of 150ug/ml to a final concentration of 8 ng/uL in 25 ml?
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- The GC content of the DNA in the genome of a newly discovered bacterium is 46%, what is the percentage of Thymine in the genome this organism?arrow_forwardThe SDS-PAGE gel matrix used to separate proteins by electrophoresis is composed of: A) Nitrocellulose B) Polyacrylamide C) Agarosearrow_forwardYou have a 2.5 ng/uL DNA stock. What volume will give you 1.15 mg?arrow_forward
- With an A260 value of 1.12 and a DNA concentration of 56.0 ng/mL, what is the pathlength of the optical system used within the Nanodrop?arrow_forwardwhen pure hemoglobin (molecular mass of 64,000Da)is separated by the following methods ,what major molecular mass species do you expect to see? explain with one sentence. gel filteration : gel filtration with 6M urea: SDS-PAGE with DTT: SDS-PAGE:arrow_forwardGradient PAGE uses differing gel concentrations along the length of the gel to achieve optimalseparation of proteins of a wide range of molecular weight proteins. How does the staining pattern of these types of gels differ from nongradient PAGE?arrow_forward
- I was trying to set this problem where the units cancel out. You are given ampicillin, with a recommended dose of 35 mg/kg , to a child with a mass of 21 kg. If stock on hand is 250 mg/ capsule, how many capsules should be given. How should I set this conversion problem up and it's also asking to express the number of capsules as an integerarrow_forwardHow many uL of DNA would I load into my gel if I am given a stock of 450ug/mL and I want to load 4ug?arrow_forwardQuantity of total DNA in a 5ul sample: Absorbance at 260nm 0.002 and at 280nm = 0.0011arrow_forward
- you are ready to elute your MBP-BAP protein from the column using 1.5mL of TBS + 10mM maltose. Create a buffer from the provided 1x TBS and 500mM maltose stock solution.arrow_forwardAfter properly troubleshooting, you finally are able to visualize the gel. You obtained the image below. The ladder used is “Lambda Hind III”.Knowing that the ladder “Lambda Hind III” consists of six fragments (23130 bp, 9416 bp, 6557 bp, 4361 bp, 2322 bp, 2027 bp), properly label the bands in the gel image.arrow_forwardYou have been thinking about heroes throughout this unit. But Beowulf, Hektor, and Achilleus are all ancient heroes. They represent cultures that flourished thousands of years ago. Do today’s heroes look anything like the heroes in these ancient tales?The characteristics of the epic heroes that you studied in this unit include the following: The hero has special qualities and is called into an adventure. The hero faces a significant battle, challenge, or obstacle. The hero is the recipient of supernatural aid (or the victim of supernatural hindrance). What traits do all heroes share? Who would be a hero today based on the characteristics of an epic hero? Do you notice any specific differences between heroes of the past and present?arrow_forward
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