After running our protein samples on an SDS-PAGE gel, we will use a Coomassie-based dye (Gel Code Blue) to stain the gel. Based on your prior experience with Coomassie dye and understanding of its function, what do you predict this dye to be able to stain? A) Only protein tags B) Only DHFR protein C) Only recombinant proteins D) All proteins

Explain the function of stacking gel, separating gel, and Coomassie blue stain in the electrophoresis experiment.

What is the difference and compare the light source and path of light of a immunofluorescence microscope from a compound light microscope?

The SDS-PAGE gel matrix used to separate proteins by electrophoresis is composed of: A) Nitrocellulose B) Polyacrylamide C) Agarose

With an A260 value of 1.12 and a DNA concentration of 56.0 ng/mL, what is the pathlength of the optical system used within the Nanodrop?

If you are using sensitivity and specificity to evaluate how well a dichotomous test (like a field sobriety test or home pregnancy test) is working, ideally what do you hope to see? Choose the correct answer and explaing why:     a) Low specificity and low sensitivity.            b) High specificity and high sensitivity.            c) Low specificity and high sensitivity.            d)_High specificity and low sensitivity.

Electrophoresis is an extremely useful procedure when applied to analysis of nucleic acids as it can resolve molecules of different sizes with relative ease and accuracy. Large molecules migrate more slowly than small molecules in agarose gels. However, the fact that nucleic acids of the same length may exist in a variety of conformations can often complicate the interpretation of electrophoretic separations. For instance, when a single species of a bacterial plasmid is isolated from cells, the individual plasmids may exist in three forms (depending on the genotype of their host and conditions of isolation): superhelical/supercoiled (form I), nicked/ open circle (form II), and linear (form III). Form I is compact and very tightly coiled, with both DNA strands continuous. Form II exists as a loose circle because one of the two DNA strands has been broken, thus releasing the supercoil. All three have the same mass, but each will migrate at a different rate through a gel. Based on your…

Is an SDS-PAGE gel, what characteristic is used to separate the proteins?  Which proteins move quickly? Which ones move slowly?

Why is it important to be able to accurately measure protein content in a sample? For example, by using different methods such as colorimetric assays and UV assays.

Why is it incorrect to say that the GFP is a “staining” method?