After running our protein samples on an SDS-PAGE gel, we will use a Coomassie-based dye (Gel Code Blue) to stain the gel. Based on your prior experience with Coomassie dye and understanding of its function, what do you predict this dye to be able to stain? A) Only protein tags B) Only DHFR protein C) Only recombinant proteins D) All proteins

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A: In biuret test, cupric ions from copper sulphate solutions in an alkaline medium forms coordinate…

Q: What is replica plating?

A:

Q: Why is the order of separation based on size opposite for gel filtration and gel electrophoresis,…

A: Gel filtration and gel electrophoresis are biochemical techniques used for the separation of…

Q: Ethidium bromide used to be a common stain for detecting DNA/ RNA on agarose gel. (i) Describe how…

A: Ethidium Bromide is a DNA stain as well as a mutagen. This dye can be visualized under UV light. It…

Q: Why is polyacrylamide gel is used in electrophoresis?

A: Introduction  Electrophoresis is a widely used laboratory technique for separating and analyzing…

Q: When you run a sequencing reaction, you should include: a) only dideoxynucleoside triphosphates…

A: Ans- When you run a sequencing reaction, you should include a higher concentration of…

Q: Which of the following is/are (a) method(s) for identifying proteins?   A  ELISA B  Eastern blot…

A: Proteins are unbranched polymers constructed from 20 standard α-amino acids. They have four levels…

Q: The diffraction limit describes the limitationof light microscopes to distinguish objects less than…

A: Combining image gathering with computationally intensive image analysis can yield a wealth of…

Q: Among various dye-based assays like Biuret, Lowry, Bicinchronic Acid, and Biuret Protein Assays,…

A: Biuret, lowry, and bicinchoninic acid-based assays are used to quantify the protein concentration in…

Q: Transmission Electron Microscopes allow us to see structures at a high resolution. When do we use a…

A: INTRODUCTION Fluorescence microscope The fluorescence microscope is any microscope that use…

Q: ii) In order to create a western blot, SDS-PAGE is first performed to separate the proteins. After…

A: SDS-PAGE is a protein separation procedure, most widely used in immunoblotting. It can be done once…

Q: What is gel electrophoresis and why is it important? Write in detail.

A: Electrophoresis is the technique that is used to separate biomolecules that have a charge in them.…

Q: Consider the following experiment. First, large populations of two mutant strains of Escherichia…

A: Mutation  The change in DNA sequence by either deletion, addition, duplication, transversion, or…

Q: You perform a Gram staining technique on two bacterial species and observe that one is stained…

A: Gram staining is a technique used to differentiate between two groups of bacteria namely…

Q: What is the application of Gel Electrophoresis in healthcare? Cite at least 2 specific examples.

A: Gel Electrophoresis: A laboratory technique called gel electrophoresis is employed to divide…

Q: Question: Define time cycled pressure limited ventilation. List the initial ventilator settings used…

A: In accordance with predetermined time cycles along with pressure restrictions, time-cycled…

Q: Why should there be 3 banding techniques (G, Q, and R)? Aside from their differences in appearance…

A: Banding techniques are utilized to understand the staining effects in the chromosomes so that the…

Q: In size exclusion chromatography, which component will elute last in the set up?   Group of answer…

A: Size exclusion chromatography is also known as gel filtration chromatography (GFC) or gel permeation…

Q: What is G-banding? O A) Most commonly used banding method B) Uses giemsa dye O C) Produces…

A: A chromosome contains the genetic material, it is present in the nucleus in condensed form.

Q: Select the sequences that would be recognized by a restriction enzyme? (There can be more than one…

A: Restriction enzymes are a group of enzymes that can cut double-stranded DNA. They are endonucleases…

Q: In hybridization method, which of the following is pressed onto agar?a) Silicon paperb)…

A: Hybridization method is a standard technique in the molecular biology. Hybridization is the…

Q: The equilibration step is adding the serum sample to the buffer-equilibrated (pH 6) magnetic Protein…

A: Immunoglobulins are glycoproteins that is purified using protein G. Protein G has specific site for…

Q: Analysis of a 49kDA protein is aimed with a western blot technique. For this purpose, "whole cell…

A: Western blot is a technique in which the concentration of a protein is determined using SDS PAGE…

Q: ) What is the advantage of cryo-sectioning for antibody staining methods?  5) When preparing…

A: Immunohistochemistry can be done in frozen sections or in paraffin-embedded sections. Both frozen…

Q: We have two specific strains of E. coli that have shown horizontal gene transfer (HGT) when mixed.…

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Q: Ethidium bromide used to be a common stain for detecting DNA/ RNA on agarose gel. (i) Describc how…

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Q: In the assay of citric acid, what do you think about the nature of solution sample ? a) Before…

A: In the citric acid assay, the concentration of the citric acid is used to determine with the help of…

Q: What do you mean by fluorescent-labeled probes?

A: Fluorescent marking is the interaction of covalently binding fluorescent colors to biomolecules,…

Q: If your analysis identifies that the total Cytosine content in a sample is 29%, what will be the…

A: According to Chargaff's rule number of purine(adenine+guanine) = number of pyrimidine (cytosine +…

Q: The following questions refer to membrane protein isolation A) What is the difficulty when working…

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Q: Which type of supercoiling takes the form of extended right-handed coils? a) Positive supercoiling…

A: Supercoiling refers to the twisting and coiling of DNA strands upon themselves, and it is a…

Q: Electrophoresis is a method used to sort proteins by their size. Why is a detergent added to the…

A: Electrophoresis  It is a biochemical technique to separate charged molecules under the influence of…

Q: Proteins secreted by bacteria can be used as biomarkers for diagnostics. Give a concise account of…

A: The identification of protein antigens from pathogens can serve as a diagnostic tool. Several…

Q: What can be done with the food dye to utilize it as an additive biocompatible material to improve…

A: 3D bioprinting offers great potential to fabricate highly complex constructs with precise control of…

Q: In fluorescent Sanger DNA Sequencing, choose one of the following: A):The deoxynucleotides are…

A: Sanger method of DNA sequencing is a method of sequencing DNA by incorporation of modified chain…

Q: In the Hershey–Chase experiment, the radioactive label 32P was present inside bacterial cells (i.e.,…

A: DNA is an organic molecule that transmits information from generation to generation. Several…

Q: Considering the technologies that pave the way for the identification of molecules on the basis of…

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Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.