RNA Inducer polymerase Activator (active) Transcription normally off. Inducer helps turn transcription on. Activator (inactive) Activator- Promoter binding site Transcription RNA polymerase cannot bind to the promoter unless the activator is bound to the activator-binding site, but the activator is in an inactive form. Inducer binds to the activator and changes its shape, allowing the activator to bind to the site. RNA polymerase can then bind to the promoter and initiate transcription. FIGURE 7.22 Transcriptional Regulation by Activators

RNA Inducer polymerase Activator (active) Transcription normally off. Inducer helps turn transcription on. Activator (inactive) Activator- Promoter binding site Transcription RNA polymerase cannot bind to the promoter unless the activator is bound to the activator-binding site, but the activator is in an inactive form. Inducer binds to the activator and changes its shape, allowing the activator to bind to the site. RNA polymerase can then bind to the promoter and initiate transcription. FIGURE 7.22 Transcriptional Regulation by Activators

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter29: Transcription And The Regulation Of Gene Expression

Section: Chapter Questions

Problem 3P: Substrate Binding by RNA Polymerase RNA polymerase has t binding sites for ribonucleoside...

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How do activators facilitate transcription? Activator (inactive) Activator- binding site RNA polymerase Promoter Transcription normally off. RNA polymerase cannot bind to the promoter unless the activator is bound to the activator-binding site, but the activator is in an inactive form. Inducer Activator (active) Inducer helps turn transcription on. Transcription Inducer binds to the activator and changes its shape, allowing the activator to bind to the site. RNA polymerase can then bind to the promoter and initiate transcription.
Editing during DNA replication is provided by DNA polymerase enzymatic activity of O 5'-> 3' polymerase 3'-> 5' polymerase O 3'-> 5' exonuclease O an extra enzyme that binds to DNA polymerase O 5'-> 3'exonuclease
Please match the gene element with the the correct definition. control elements 5' UTR 3' UTR Promoter coding region +1 site [Choose ] stretch of DNA that contains the sequence that will encode the protein the active form of RNA polymerase region of DNA upstream of transcription start site that is recognized by RNA polymerase region of mRNA on the 5' end that goes untranslated RNA polymerase and the sigma factor regions of DNA that increase or decrease transcription rate the first nucleotide to be transcribed region of mRNA on the 3' end that goes untranslated a transcription initiation factor that helps RNA polymerase recognize the promoter the inactive form of RNA polymerase [Choose ] [Choose ]
When CAMP binds to the Catabolite Activator Protein (CAP), it causes a conformational change on the protein, which results in binding to the CAP- binding site on the DNA and molecule is known as a(n) of the lac operon. This conformational change induced by the binding of another transcription activation; allosteric effect transcription repression; binding effect translation; distortion effect mutation; interactive effect attenuation; compression effect O O O O
Describe transcriptional components in E. coli for each step of transcription, explain each subunit of RNA polymerase, promoter region, initiation stage, the reaction involved in elongation during RNA synthesis, any important proteins, and termination stage
Listed below are steps in the transcription process. Reorganize the list so the steps in the correct order- starting with the first step in initiating transcription and ending with completion of a new strand of RNA (in other words- from start to finish of transcription). RNA polymerase reaches the termination signal DNA unwinds underneath RNA polymerase at transcription start site RNA polymerase is recruited to the promoter region mRNA transcript is released General Transcription factors bind TATA box (and other DNA sequences) in the promoter region General Transcription Factors unbind from promoter region mRNA transcript synthesis occurs RNA polymerase moves along the template strand in the 3’ to 5’ direction
In rho dependent transcription termination the rho factor binds to? You have a 18 ml sample of acetylcholine a neurotransmitter with an unknown concentration and a ph of 7.72 you incubate this sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid the acetic acid dissociates to yield acetate and hydrogen ions at the end of the incubation period you measure the ph again and find that it has decreased to 6.32 assuming there was no buffer in the assay mixture determine the num? For each genotype only a corresponding phenotype can be expressed the chromosomes in the zygote are not exact copies of those in the mother s ovaries and the father s testes ?
Use the illustration as a reference to select the correct statements transcription factors of eukaryotic cells 00 Other transcription factor proteins Gene Enhancers Promoter 3 This protein complex makes it easier for RNA polymerase to attach to the promoter and start transcribing a gene 1 Activator proteins bind to pieces of DNA called enhancers. Their binding causes the DNA to bend, bringing them near a gene promoter, even though they may be thousands of base pairs away. RNA polymerase Activator proteins note This dagram simplifies the DNA - A 4 An insulator can stop the enhancers from binding to the promoter, if a protein called CTCF (named for the sequence CCCTC, which occurs in all insulators) binds to it. 2 Other transcription factor proteins join the activator proteins, forming a protein complex which binds to the gene promoter. Methyl groups CTCF (CCCTC-binding factor) -Insulator 5 Methylation, the addition of a methyl group to the C nudeotides, prevents CTCF ulator, enhancers…
iestiðn Pre-initiation complex (PIC) is formed right before the initiation of genes, which of the following steps seemes to NOT help for the PIC formation? O TBP protein to recognize TATA box at promoter region O TFIIA to recognize the TAFS in TFIID O Enhancers to attract coactivators and activators O Silencers to attract repressor and corepressors
Substrate Binding by RNA Polymerase RNA polymerase has t binding sites for ribonucleoside triphosphates: the initiation site and the elongation site. The initiation site has a greater Km for NTPs than the elongation site. Suggest what possible significance this fact might have for the control of transcription in cells.
Identify the DNA elements and protein factors, #1-7, in the figure below that are involved in the initiation and elongation of transcription at a eukaryotic promoter of a gene TRANSCRIPTION ON RNA PROCESSING PefA A eukaryotic promoter commonly includes a TATA box (a nucleotide sequence TRANSLATION Ritoom containing TATA) about 25 nucleotides upstream from Nontemplate (coding) strand of DNA 7 Pupeptide 1 the transcriptional start point. Nontemplate (coding or sense) strand DNA TATAAAA ATATILTI 5 2 3 Start point 2 Several transcription TCCAA 3' 5' factors, one recognizing the TATA box, must bind 3' end to the DNA before RNA 4 polymerase Il can bind in the correct position and orientation. UCCA 3 5' 3' 3 Additional transcription GGTT factors (purple) bind to the DNA along with RNA 5' Direction of transcription polymerase II, forming the transcription initiation 3 complex. RNA polymerase Il then unwinds the DNA double helix, and RNA synthesis begins at the start point on the temple strand.…
Please help, doesn't it usually start before the promoter or after, please just give me a brief explanation