How do activators facilitate transcription? Activator (inactive) Activator- binding site RNA polymerase Promoter Transcription normally off. RNA polymerase cannot bind to the promoter unless the activator is bound to the activator-binding site, but the activator is in an inactive form. Inducer Activator (active) Inducer helps turn transcription on. NNNNA Transcription Inducer binds to the activator and changes its shape, allowing the activator to bind to the site. RNA polymerase can then bind to the promoter and initiate transcription.

TFIID TFIIA TFIIH TBP TFIIB TFIIF TFIIE

The basic of transcription include nucleosome remolding, pre-initiation, initiation, promoter clearance and elongation and termination. Describe these steps into detail including basic machinery involved, enzymatic activities and potential aberrancies that increase or decrease gene transcription

Which of these are genetic regulatory proteins and which are small effector molecules?

When CAMP binds to the Catabolite Activator Protein (CAP), it causes a conformational change on the protein, which results in binding to the CAP- binding site on the DNA and molecule is known as a(n) of the lac operon. This conformational change induced by the binding of another transcription activation; allosteric effect transcription repression; binding effect translation; distortion effect mutation; interactive effect attenuation; compression effect O O O O

Identify the DNA elements and protein factors, #1-7, in the figure below that are involved in the initiation and elongation of transcription at a eukaryotic promoter of a gene TRANSCRIPTION ON RNA PROCESSING PefA A eukaryotic promoter commonly includes a TATA box (a nucleotide sequence TRANSLATION Ritoom containing TATA) about 25 nucleotides upstream from Nontemplate (coding) strand of DNA 7 Pupeptide 1 the transcriptional start point. Nontemplate (coding or sense) strand DNA TATAAAA ATATILTI 5 2 3 Start point 2 Several transcription TCCAA 3' 5' factors, one recognizing the TATA box, must bind 3' end to the DNA before RNA 4 polymerase Il can bind in the correct position and orientation. UCCA 3 5' 3' 3 Additional transcription GGTT factors (purple) bind to the DNA along with RNA 5' Direction of transcription polymerase II, forming the transcription initiation 3 complex. RNA polymerase Il then unwinds the DNA double helix, and RNA synthesis begins at the start point on the temple strand.…

Which basal transcription factor contains a subunit that causes local distortion in DNA so that proteins can assemble to the promoter? TFIIA TFIID TELIH TFUIF

A bacterial species has a hypothetical sigma promoter that has the following sequence: TTGGCA - 18 bases - TATAAT What change in the level of transcription would there be if the sequence was mutated to: TTCGCA -18 bases -TATAAT O The mutation would bind the promoter to the consensus and produce normal levels of transcription O The mutation would inhibit the promoter thereby inhibiting transcription The mutation would move the promoter away from consensus and reduce the level of transcription O No change the consensus TATAAT sequence in the same. D00 F3 F4 F5 F6 F7 F8 F9 % & 5 6 7 8 9 %24

iestiðn Pre-initiation complex (PIC) is formed right before the initiation of genes, which of the following steps seemes to NOT help for the PIC formation? O TBP protein to recognize TATA box at promoter region O TFIIA to recognize the TAFS in TFIID O Enhancers to attract coactivators and activators O Silencers to attract repressor and corepressors

TAN TTGC IGGAG Nanog regulatory sequence Given this interaction map which DNA sequence would the transcription factor most likely bind to? CAAGGAG ATTAACG TAATTGG TAATTGC