PROOFREADING NEW DNA DNA polymerase initially makes about 1 in 10,000 base pairing errors Enzymes proofread and correct these mistakes - The new error rate for DNA that has been proofread is 1 in 1 billion base pairing errors
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- Protein Synthesis and Mutation Practice • Complete the lines below by determining the mRNA transcript and amino acid sequence. • Compare the mutant DNA strands to the wild type strand. ⚫ Circle the mutation in the mutant DNA strands and describe the type of mutation (frameshift - insertion, frameshift - deletion, point - missense, point - silent, or point-nonsense). Not all of these will be used in this assignment! Wild type DNA template: 3' TACGCGTGCACGATGCAGTAGTACATC5' mRNA transcript sequence: Amino acid sequence: Mutation #1 DNA template: 3' TACGCGTGCACGATCCAGTAGTACATC5' mRNA transcript sequence: Amino acid sequence: Type of mutation: Mutation #2 DNA template: 3' TACGCGTGCTCGATGCAGTAGTACATC5' mRNA transcript sequence: Amino acid sequence: Type of mutation:Home Work: • Suppose you perform a PCR that begins with one double-strand of the following DNA template: +5' -СТАССТСCGGGTTGACTGСТАССТТССССGGATGCCCAAAAТТСТСGAG-3— :::::::::::: :::::::::::: :::: +3'-GATGGACССССААСТGACGATGGAAGGGCCCТАССGGTTTTAAGAGCTC-5'+ A. Draw one cycle of PCR reaction below the following diagram. B. Label the template DNA, the primers, and what is happening at each step. (1) température cycle #1Updates In gel electrophoresis, the smallest DNA fragments will travel the farthest. Why Grades does this consistently occur?* Members O Small fragments have less charge on them and therefore travel farther. Conferences Small fragments are the first to leave the well and have more time to travel than the DBQ Online larger fragments. Jewsela O The higher molecular weight of larger fragments make them sink. mation O Small fragments move more freely through the agar gel. logy Periods 1 and 2 s periods nool MP1, Highschool
- Save On 13 2 Manipulating DNA pages 322_323 task - Last Modified: Mon at 1:11 PM - Makama, Aisha B. MA Home Insert Draw Design Layout References Mailings Review View Help A Share 1) Develop an analogy for the processes researchers use to make changes to DNA. In your analogy, explain how it is similar to the techniques used in genetic engineering. You can draw a graphic organizer, make a table, or write a few sentences describing your analogy. 2) Devise flowchart that shows the steps to prepare DNA for gel electrophoresis, as well as the protocol for setting up and running a gel. You can add diagrams to the flowchart and add detailed notes if you like. DFocus 9:46 PM 2122/2021 135 words English (United States) Page 1 of 1 P Type here to searchQ1 Homework • Unanswered Q1. I want to do a PCR experiment that requires a final concentration of 2 uM of primers. If my primer stock solution is 10 uM, and my reaction volume is 25 ul, how much primer solution do I add? Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a 1 ul 2.5 ul 5 ul d 10 ul 5.1. What is the difference between an iterated blast (psi-blast) search and a simple blastsearch?2. Arslan sequenced a gene in lab how he will know about the gene either it is noveldiscovery or gene is already present in the database?3. Ruwaifa want to compares a nucleotide query sequence what option he will opt inBLAST and why?4. Uzair has two protein sequences, he want to check their similarities what he will do? What results are expected
- Select all that are true about DNA gel electrophoresis O This technique can be used to separate DNA based on the size of the fragment O Since DNA is positively charged it will travel to the negative electrode O After a gel is run, the larger fragments end up being further away from the well The rate at which DNA fragments travel is logarithmically and is inversely proportional to its sizeUsing the skills you learnt in the DNA Analysis tutorial, correctly identify the gene and the species of the following DNA sequence: AACCAGTGTGCTGCAGGCTGCACAGGCCCCCGGGAGAGCGACTGCCTGGTCTGCCGCAAA Gene: DNA Polymerase II. Species: Mus Musculus Gene: RNA Polymerase I. Species: Homo Sapiens Gene: Insulin Receptor (INSR). Species: Homo Sapiens Gene: Epidermal Growth Factor Receptor (EGFR). Species: Homo SapiensLab 10- PCR For the following equipment, know what it looks like, its name, and its function: micropipette, tip, water bath, centrifuge, thermal cycler For the following solutions, know its name and its function: saline, InstaGene, MasterMix What's the difference between pv92 and Alu? On what chromosome is pv93 located? What are the names of and general functions of the 3 steps to PCR (denature, anneal, extend)? What is the relevance of the 2 different stop points of the plunger on a micropipette? What was the importance of primers and Taq? What was the cofactor we tried to take away from DNase yet later gave to Taq?
- • Suppose you perform a PCR that begins with one double-strand of the following DNA template: +5'-CTACCTGcoGOTTGACTOCTACCTTcccaGGATaccCAAAArTCTOGAG-3 +3-GATOGACOCCAACTGACGATGGAMGCCCTACOOUTITTAAGGCTC-S'+ A. Draw one cycle of PCR reaction below the following diagram. B. Label the template DNA, the primers, and what is happening at each step. temprature (2) eynteeLO 64- Explain how Next gen sequencing is applied in different technologies What is the benefit of using Next-Gen sequencing instead of Sanger sequencing? You can use fluorescent tags It uses reversible terminators called ddNTPs Faster and more copies can be sequenced at a time It uses irreversible terminators called ddNTPs It allows you to identify the nucleotides of a short DNA sequenceDNA Profiles as Tools for Identification A PCR-based paternity test is conducted using STRs that consistently produce a unique DNA fragment pattern from a single chromosome. Examining the results of the following Southern blot, which male(s) can be excluded as the father of the child? Which male(s) could be the father of the child?
