Instructions Your task in this section is to build a bacterial replisome at the replication fork. Drag and drop components of the replication machinery onto the DNA. Components may be used more than once.
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- Hello, I would really appiarte help appreciate help. This is a blank question so I am unsure why it was rejected immeditely the first time. Thank you in advance. Identify the type of mutation and how it would affect the protein made (amino acid) if the following changes occurred in the DNA antisense strand First codon change from TAC to TAT. Third codon change from ACG to ACA. Ninth nucleotide changes from G to T. Nucleotide with adenine (A) base inserted between 3rd and 4th nucleotide. Types of Mutation Changes in the Amino Acid 1. 2. 3. 4.The following statements are either accurate as written or contain some errors. You need to rewrite each as an accurate statement. Make sure that you do not change the material the sentence is about. Highlight or underline any changes that you make to statements. Thank you! 1. Base Excision repair happens during replication and is when an incorrect nucleotide is incorporated into the DNA. During this type of repair the damaged or incorrect base is removed and fixed via Nick translation. 2. There are four levels of polypeptide structure: primary, secondary, tertiary, and quaternary. The secondary structure describes the linear sequence of the proteins in the polypeptide. 3. The majority of proteins fold spontaneously when they come out of the ribosome after translation. The reaming proteins need help from chaperones OR chaperonins to fold properly.☆回 w Insert Format Tools Add-ons Help Last edit was yesterday at 8:08 AM TURN 100% Normal text Arial 11 BIUA E- 1E E E - E EX 2. 3 . 5. C Directions: Describe the process of DNA replication in the space below. Specifically reference the pictures in your answer. Your response will undergo a plagiarism check. Be sure to write your response in your own words. Be sure to reference and thoroughly explain: The relationship and importance of DNA replication and the cell cycle. The conservation of genetic information during replication. (Is it conservative? Semi-conservation? Dispersive? What does this mean?) The specific enzymes used in each step and indicate whether each of the enzymes is a synthesis reaction or a digestion reaction. Justify your answer through explanation. Identify the parameters and limitations of the enzymes working effectively. (What factors would limit or interfere with DNA replication?)
- Please answer asap and type your answer and do not copy from anywhere please NOTE*** double stranded complementary DNA I have determined is GTCATACCTAGGGTA with a palindromic site of CCTAGG and the enzyme BamHI that will cut the recognition site. In the double-stranded DNA you have determined, there is also another recognition site for common restriction enzymes. Which enzyme would cut your DNA at this other recognition site? (Hint: Remember that one recognition site can overlap with or be completely contained by the sequence containing another recognition site.) HindIII Sau3AI AluI BamHIInstructions: Read 13-2 Manipulating DNA pages 322-323. As you read each section, examine the figures and captions (explanations). Identify any questions you may have. 1) Develop an analogy for the processes researchers use to make changes to DNA. In yo analogy, explain how it is similar to the techniques used in genetic engineering. You can draw a graphic organizer, make a table, or write a few sentences describing your analogy. 2) Devise flowchart that shows the steps to prepare DNA for gel electrophoresis, as well the protocol for setting up and running a gel. You can add diagrams to the flowchart an add detailed notes if you like. English (inited Sate) O Focs ere to search 4 CO RU G\ L B. 2N A\ Alt Ciri08 Nucleoside and nucleotide analogs are two closely related classes of anti-viral drugs that inhibit viral DNA or RNA polymerase enzymes. What is their most common mechanism of action and why are they often referred to as "chain terminators"? Edit View Insert Format Tools Table A xd0 Paragraph v They are referred as chain terminators because the sturcutre when looked at does not contain a 3 prime end 31 8 8:10 EE O 6 n
- Can you please check my answer and make sure it is correct. Question: List the ingredients of master mix, and state the purpose of each ingredient. Answer: Taq DNA polymerase This enzyme synthesizes the complementary strand of the DNA template after attaching to the primer. This means that it adds on free nucleotides to the existing strand, but helps speed up the covalent bonding between these newly added nucleotides. This enzyme is also thermally stable meaning that it can withstand the hot temperatures needed for PCR to occur. This hot temperature is needed for the denaturation step when the double stranded DNA has to be unwound and separated into two strands. Individual building blocks of DNA (either free nucleotides A, T, C, and G or dNTP’s) These nucleotides are needed to build the complementary strand of DNA A special buffer to maintain the optimal pH, salts, and MgCl2 These buffers help maintain a good pH that doesn’t become too acidic or basic for Taq DNA polymerase to…Figure 9-22 shows the first steps in the process of making a DNA microarray, or DNA chip, using photolithography. Describe the remaining steps needed to obtain the desired sequences (a different fournucleotide sequence on each of the four spots) shown in the first panel of the figure. After each step, give the resulting nucleotide sequence attached at each spot.Your answers are saved automatically. Remaining Time: 1 hour, 08 minutes, 45 seconds. * Question Completion Status: A Moving to another question will save this response. Question 17 True/False Question (suggested time - up to 1 minute): DNA primase synthesizes a short RNA primer that later appears at the 5'-end of each Okazaki fragment. O True O False A Moving to another question will save this response. lyp
- to 4 minutes) The schematic diagram below shows organization of the DNA replication fork. Match parts of the diagram (labeled A-F) with the corresponding term from the answer list (designated 31 parental duplex 5' 3' fork progression v A 1Lagging strand 2. An Okazaki tragment 3.Site of action of DNA topoisomerase 4 Leading strand 5. Site of action of DNA helicCase 6.Site of action of DNA ligaseDNA Replication, Sequencing, and Recombination - PowerPoint Karla Henthorn KH Animations Slide Show Review View Help O Tell me what you want to do A A E. s et Direction- Align Text Shape Fill O Find US abs AV- Aa - ay. A- Stiape Outine- ab Replace- ne Arrange Quick Styles Convert to SmartArt Shape Effects A Se Font Paragraph Drawing 6 5 4 1 3 Ec 2 1 2 3 H4 Kim ACTIVITY Consider the following DNA molecule: ACG GTACACTTA C G A A Henthorn, ic T GCCAT GT GA ATG CT T Rc 1) Draw the 2 strands after they have "unzipped". 2) Follow the rules for pairing and fill in the new strands with the correct nitrogenous bases. Write these new strands in a different color of ink. = Notes Comments 可 DO DELLQuestions are talking about amplification of DNA When we run the gel, we also run a ladder why? what information does that give us. why does it form a ladder shape on the gel (what is happening to cause things to separate out in many bands)?