Select all that are true about DNA gel electrophoresis O This technique can be used to separate DNA based on the size of the fragment O Since DNA is positively charged it will travel to the negative electrode O After a gel is run, the larger fragments end up being further away from the well Ltl. D LA C

Updates In gel electrophoresis, the smallest DNA fragments will travel the farthest. Why Grades does this consistently occur?* Members O Small fragments have less charge on them and therefore travel farther. Conferences Small fragments are the first to leave the well and have more time to travel than the DBQ Online larger fragments. Jewsela O The higher molecular weight of larger fragments make them sink. mation O Small fragments move more freely through the agar gel. logy Periods 1 and 2 s periods nool MP1, Highschool

Incorrect 1 A Help Center? It can sequence a whole genome in one huge strand Each nucleotide has a different marker attached Question 11 LO62 Explain how SANGER sequencing works Order the steps that are needed for gene sequencing through Sanger technology: Amplify DNA Cut DNA into small pieces Add DNA to be sequenced + DNA polymerase + dNTPs + ddNTPs into different test tubes Run resulting sequences through gel electrophoresis 3 111 0/1 pts 0 / 1 pts

Hind III' Hind III .9 kb .9 kb Bam HI plasmid A .9 kb .7kb Bam HI .3 kb plasmid B .9 kb .4 kb .3 kb .5 kb/ Eco RI Bgl Il .4 kb Eco RI .7 kb .5 kb -Bgl II Bam HI Eco RI You requested that a lab run by a friend of yours share a couple of plasmids that they created with you. She was willing to send you the ones that you asked for in exchange for citation in any papers that were published using the plasmids. Eco RI However, the labels from two vials have fallen off in the shipping because the adhesive used to hold them was not able to withstand the temperature, being shipped on dry ice. Each vial contained a different plasmid and now you do not know which plasmid is in which. Fortunately, you have restriction maps for both plasmids, shown above. Explain how you would determine which vial contained each plasmid and give a graphical representation of your expectations related to your method of Bam HI discovery. (25pts)