The starting concentration of the lambda DNA is usually 250 ng/μl. Biology 6 B DNA Restriction Digests, Ligations, & Electrophoresis Here's how much DNA you should use for each reaction: Uncut: 0.2 μg DNA (A small amount because it will all be in one band on the gel; if there's too much, the band will be smeared.) Convert to μl: EcoRl-cut: 1.0 μg DNA. Convert to μul: HindIII-cut: 3.0 μg DNA. (A larger amount because you're going to save some for later.) Convert to μl: EcoRI & HindIII-cut: 1.0 μg DNA. Convert to μl:

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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The starting concentration of the lambda DNA is usually 250 ng/μl.
Biology 6 B
DNA Restriction Digests, Ligations, & Electrophoresis
Here's how much DNA you should use for each reaction:
Uncut: 0.2 μg DNA (A small amount because it will all be in one band on the gel;
if there's too much, the band will be smeared.) Convert to μl:
EcoRl-cut: 1.0 μg DNA. Convert to μul:
HindIII-cut: 3.0 μg DNA. (A larger amount because you're going to save some for
later.) Convert to μl:
EcoRI & HindIII-cut: 1.0 μg DNA. Convert to μl:
Transcribed Image Text:The starting concentration of the lambda DNA is usually 250 ng/μl. Biology 6 B DNA Restriction Digests, Ligations, & Electrophoresis Here's how much DNA you should use for each reaction: Uncut: 0.2 μg DNA (A small amount because it will all be in one band on the gel; if there's too much, the band will be smeared.) Convert to μl: EcoRl-cut: 1.0 μg DNA. Convert to μul: HindIII-cut: 3.0 μg DNA. (A larger amount because you're going to save some for later.) Convert to μl: EcoRI & HindIII-cut: 1.0 μg DNA. Convert to μl:
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