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E. coli can grow at a higher temperature in a complex medium
than in a defined medium. Why?
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- Explain why a green metallic sheen is formed when E. coli is grown on Eosin Methylene Blue Agar.Why is PBS buffer preferred when preparing a monolayer cell culture?Is the telluride glycine agar? (TGA) a complex or defined medium? Explain based on its composition. What kind of Media is TGA explaining based on what kind of microorganisms it allows to grow?
- Helicobacter pylori, a bacterium that causes stomach ulcers, can grow in medium with a pH above 5. It can survive but not grow in pH 2 medium as long as the medium contains Urea. You inoculate a pH 2 broth with 1,000,000 H. pylori and for 8 hours the organism did not grow. The next morning however, the medium has become quite turbid, suggesting that the organism has grown. Explain how this could happen. What phylum and class does this organism belong?Why do cells need to be kept in the freezing devices first then transferred to liquid nitrogen storage in crypto preservation? In addition, why are antibiotics not used in the cell freezing media? Why FBS concentration higher than 10% (10% is the standard concentration in complete growth media) is recommended in cell freezing media?In growing E.coli, why is that (reasons) they do not grow after doubling time under 20 degrees celsius and under 37 degrees celsius, the number of colonies after doubling time has decreased?
- Under what conditions are media typically sterilized in an autoclave?The results of Figure 2 highlights the antibacterial activity of cacay oil. Since the method involves adding cacay oil to a "broth" medium, it can be inferred that cacay oil is hydrophilic. Agree or Not?In growing E.coli, why is that (reasons) they do not grow after doubling time under 20 degrees celsius and under 37 degrees celsius, the number of colonies after doubling time has decreased? provide reliable sources (links).
- Escherichia coli but not Pyrolobus fumarii will grow at 40°C,while P. fumarii but not E. coli will grow at 110°C. What ishappening (or not happening) to prevent growth of eachorganism at the nonpermissive temperature?Since E. coli normally grows in the human gut, speculate on why its optimal growth temperature is different from the commonly cited temperature of a normal healthy human -37°C.Would you expect a heterotrophic organism to grow in an inorganicsynthetic medium? Explain.