Nitrogen fixing bacteria can use atmospheric nitrogen as their sole source of nitrogen. Imagine you are trying to select only nitrogen fixing bacteria from a soil sample. Could the sample be grown on a complex medium? Explain why or why not.
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Nitrogen fixing bacteria can use atmospheric nitrogen as their sole source of nitrogen. Imagine you are trying to select only nitrogen fixing bacteria from a soil sample. Could the sample be grown on a complex medium? Explain why or why not.
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- On which of the following types of media would you expect untransformed bacteria to grow on? LB agar LB agar with ampicillin Untransformed bacteria will not grow on any type of medium. Untransformed bacteria will grow on all three types of media. LB agar with ampicillin and arabinoseA soil sample is placed in liquid and the number of bacteria in the sample determined in two ways: (1) colony count and (2) direct microscopic count. How would the results compare?a) Methods 1 and 2 would give approximately the same results.b) Many more bacteria would be estimated by method 1.c) Many more bacteria would be estimated by method 2.d) Depending on the soil sample, sometimes method 1 would be higher and sometimes method 2 would be higher.). Consider a culture medium on which only gram-positive organisms such as Staphylococcus aureus colonies can grow due to an elevated NaCl level. A yellow halo surrounds the growth, indicating the bacterium fermented sugar in the medium, decreasing the pH as a result and changing the color of a pH indicator chemical. This type of medium would be referred to as a(n): A) Selective medium. B) Differential medium. C) Enrichment culture. D) Selective and differential medium. E) Differential and enrichment culture.
- We are looking for bacteria that can grow with ribose sugar as the only carbon source. Several specimens of bacteria have been sampled from the soil. Pure cultures from each sample are plated on general purpose media (which includes commonly used sugars like glucose), and onto plates containing only ribose as a carbon souce. Which of the following is true? (Consider the entire statement.) The ribose plates are selective - the bacterial colonies on these plates can be compared to those growing on the general purpose plates to look for differences in colony appearance. The ribose plates are differential - the bacterial colonies on these plates can be compared to those growing on the general purpose plates to look for differences in colony appearance. The ribose plates are differential - only bacterial colonies able to use ribose as a carbon source will grow on those plates. The ribose plates are selective - only bacterial colonies able to use ribose…In August 2021, there was a multi-state outbreak of melioidosis, an infectious disease caused by the bacterium Burkholderia pseudomallei, thought to arise from imported products. Burkholderia pseudomallei can be grown in culture. In terms of human health, why might it be critical to grow a pure culture of bacteria?Your lab coordinator has decided to grow and maintain a sourdough starter. A sourdough starter is a live, on-going culture of yeast that is grown in flour, water, and a bit of sugar. It is used as an ingredient in some types of bread. This is the question : How does it accumulcate another species? You have been asked to take this mixed culture and isolate the different species in the starter (which is basically a liquid culture). How will you do it?
- After inoculating and incubating an agar slant from a pure broth culture of a bacterial species such as E. coli, which of the following would indicate an unsuccessful aseptic transfer? (Choose ALL that apply) a - There is fungal growth in the original broth culture tube. b- There is too much growth on the agar slant. c- There are colonies of similar morphology on the slant. d - There are red, yellow, and white colonies on the slant. e - There is no growth on the slant.Mannitol salt agar is often used to distinguish between different species of Staphylococcus, a gram positive bacterium that is well adapted to living on dry, salty skin. Disease-causing strains of Staphylococcus ferment mannitol; non-pathogenic strains cannot use mannitol. Is the medium Defined or Complex?You are characterizing a newly discovered organism . You have noticed that the organism grows on two carbon sources - sucrose and mannitol . Whenever you add both sugars to the medium , sucrose will be completely metabolized before mannitol . a ) What is this growth pattern called ? b ) Describe a possible growth curve of sequential metabolism of the sugars .
- In a biological production process, bacteria with spore-forming ability are immobilized on a support surface. Once a batch of production is done, these cells along with their support material are separated from the medium, and then sterilization is performed in a furnace for safe disposal of material into the environment. An important issue here is to ensure ALL bacteria, including any spores that may form and those that are located at the center of the spherical support material, are killed with heat. For this purpose, the temperature at the center of the spherical support material must reach the designated killing temperature. The support material is a sphere with 3 cm diameter and a heat capacity of 4.0 kJ/kg*K, a density of 800 kg/m³, a thermal conductivity of 0.7 W/m*K. The heat transfer coefficient of the surrounding air is 14 W/m2*K. If the initial temperature inside the furnace prior to the start of sterilization is 15 °C, how long should the procedure last to obtain sterile…The results of Figure 2 highlights the antibacterial activity of cacay oil. Since the method involves adding cacay oil to a "broth" medium, it can be inferred that cacay oil is hydrophilic. Agree or Not?Imagine that you have set up a Kirby Bauer experiment on Mueller Hinton agar (non-selective agar) in order to test if Escherichia coli is susceptible or resistant to Drug X. After 24 hours, you notice a zone of inhibition (clear zone) around your antibiotic disc and determine that E. coli is susceptible to Drug X. You then take a cotton swab and subculture a sample from this zone of inhibition (where no growth was apparent) onto a new agar plate without antibiotics and after 24 hours you see growth of E.coli. What can you conclude about Drug X?