What phenomenon would you expect to see occurring under a microscope when healthy cells of E. coli bacteria are placed in a dish of distilled water? Briefly explain the underlying mechanism.
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- Bacterial growth depends on many environmental factors, including the temperature of the environment. Since microbes can survive in a certain range of temperatures and will thrive at a temperature, understanding these variables allows for control of their growth. This understanding can be used, for example, to preserve certain foods or to treat infections. This lab simulation will use an instrument called a spectrophotometer. This instrument quantitatively measures the amount of light that is absorbed or transmitted by molecules in solution a) In your own words give an introduction to the microbial growth and the effect tempature has. b) In your own words explain the importance of the spectrophotometer in regards to microbial growth. c) In your own words give a hypothesis on how bacteria will react to different temperatures.How would you produce a 10^-1 dilution if a 3 mL bacterial sample using the entire 3 mL volume? suppose your professor handed you a test tube with 2.0 mL of an E. coli broth culture in it and told you to make a 10^-1 dilution of the entire culture. Explain how you would do this. Show your calculations.The images attached are the photos of bacteria in yoghurt under a misroscope. According to these images and your own knowledge, can you make a biological drawing of bacteria in yoghurt ( in a circle) and identify the types of bacteria accurately?
- You have a cell suspension in an isotonic medium. Explain in detail what would happen when you add: A) 50 mM Urea; or B) 50mM mannitol.In step 3 of today's protocol, it mentions that the incubation step with the P2 buffer should not be allowed to proceed for more than 5 minutes. a) What is the chemical composition and concentration (not mass) of each component of the P2 buffer?b) c) What is the purpose of adding the P2 buffer? What would be the major problem if this step is allowed to proceed for a long period of time?In the experiment by Bernard Davis, bacterial F+cells and F- cells were growing while separated by a filter. Filter pores allowed the passage of the liquid medium but not the bacteria cells. As a result: 1) prototype colonies grew well on minimal medium 2) F+ cells were converted to F- cells despite the physical separation 3)F- cells were converted F+ cells despite the physical separation 4)F+ cells were not converted to F- cells because of the physical separation 5) F- cells were not converted to F+ cells because of the physical separation 6)there was no growth of prototypes on minimal medium
- When viewed under a microscope, bacteria appear as red rods after they have been stained. Explain a quick process for determining the outcome of this experiment.What cell types would be able to grow on the ECM in the following situations? F- , Hfr , Mix a) Streptomycin was not added to the ECM. b) The ECM contains thiamine. c) The ECM contains all 20 amino acids and all 5 nitrogenous nucleic acid basesYou perform a Gram staining technique on two bacterial species and observe that one is stained purple while the other is stained pink. Which of the following is consistent with this observation? a) The purple one is likely more resistant to penicillin b) Both bacteria have a cell wall made of mycolic acid c) Their peptidoglycan layers have the same thickness d) They differ in the number of phospholipid membranes e) One has a monolayer of phospholipids while the other has a bilayer of phospholipids
- What general type of growth medium would you use to: (a) grow one type of bacteria but inhibit the growth of another type? (b) discriminate between different types of bacteria?The number of bacteria in culture increases rapidly. The table below gives the number of bacteria a few times (in hours) after the moment when. (a) Find the average rate of change for the number of bacteria from 0 hours to 3.1 hours. (b) Find the average rate of change for the number of bacteria from 6.2 hours to 15.5 hours.A bacterium has a generation time of 30 minutes. You transfer cells from an exponentially growing culture to a fresh source of the same medium so that the freshly inoculated medium contains 3.2 x 106 cells per milliliter. This new culture does not exhibit a lag phase. About how many cells (per milliliter) should you have after 1.5 hours incubation?? Solve this step by step and by using formula